Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08955 and BWI76_RS08960 overlap by 6 nucleotides BWI76_RS08960 and BWI76_RS08965 are separated by 110 nucleotides BWI76_RS08965 and BWI76_RS08970 overlap by 4 nucleotides
BWI76_RS08955: BWI76_RS08955 - 23S rRNA (adenine(1618)-N(6))-methyltransferase, at 1,886,653 to 1,887,567
_RS08955
BWI76_RS08960: BWI76_RS08960 - mechanosensitive channel protein, at 1,887,562 to 1,889,772
_RS08960
BWI76_RS08965: BWI76_RS08965 - glutamine ABC transporter ATP-binding protein, at 1,889,883 to 1,890,605
_RS08965
BWI76_RS08970: BWI76_RS08970 - glutamine ABC transporter permease GlnP, at 1,890,602 to 1,891,261
_RS08970
Position (kb) 1887
1888
1889
1890 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1886.682 kb on + strand at 1886.710 kb on - strand at 1886.838 kb on + strand, within BWI76_RS08955 at 1886.856 kb on - strand, within BWI76_RS08955 at 1887.157 kb on - strand, within BWI76_RS08955 at 1887.196 kb on + strand, within BWI76_RS08955 at 1887.209 kb on - strand, within BWI76_RS08955 at 1887.274 kb on - strand, within BWI76_RS08955 at 1887.274 kb on - strand, within BWI76_RS08955 at 1887.274 kb on - strand, within BWI76_RS08955 at 1887.446 kb on - strand, within BWI76_RS08955 at 1888.058 kb on + strand, within BWI76_RS08960 at 1888.343 kb on - strand, within BWI76_RS08960 at 1888.343 kb on - strand, within BWI76_RS08960 at 1888.640 kb on + strand, within BWI76_RS08960 at 1888.749 kb on + strand, within BWI76_RS08960 at 1888.839 kb on + strand, within BWI76_RS08960 at 1888.896 kb on - strand, within BWI76_RS08960 at 1889.471 kb on + strand, within BWI76_RS08960 at 1889.479 kb on - strand, within BWI76_RS08960 at 1889.803 kb on + strand at 1889.912 kb on + strand at 1890.342 kb on + strand, within BWI76_RS08965 at 1890.421 kb on - strand, within BWI76_RS08965 at 1890.461 kb on + strand, within BWI76_RS08965 at 1890.469 kb on - strand, within BWI76_RS08965 at 1890.474 kb on + strand, within BWI76_RS08965 at 1890.487 kb on - strand, within BWI76_RS08965 at 1890.645 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 1,886,682 + +0.3 1,886,710 - -0.0 1,886,838 + BWI76_RS08955 0.20 +1.8 1,886,856 - BWI76_RS08955 0.22 -0.2 1,887,157 - BWI76_RS08955 0.55 -0.6 1,887,196 + BWI76_RS08955 0.59 +0.7 1,887,209 - BWI76_RS08955 0.61 +0.0 1,887,274 - BWI76_RS08955 0.68 -2.0 1,887,274 - BWI76_RS08955 0.68 +0.2 1,887,274 - BWI76_RS08955 0.68 -1.1 1,887,446 - BWI76_RS08955 0.87 +0.5 1,888,058 + BWI76_RS08960 0.22 -1.4 1,888,343 - BWI76_RS08960 0.35 -0.0 1,888,343 - BWI76_RS08960 0.35 +0.3 1,888,640 + BWI76_RS08960 0.49 +0.3 1,888,749 + BWI76_RS08960 0.54 -0.1 1,888,839 + BWI76_RS08960 0.58 +0.6 1,888,896 - BWI76_RS08960 0.60 +0.9 1,889,471 + BWI76_RS08960 0.86 +0.3 1,889,479 - BWI76_RS08960 0.87 +0.3 1,889,803 + +0.4 1,889,912 + +0.8 1,890,342 + BWI76_RS08965 0.63 -1.1 1,890,421 - BWI76_RS08965 0.74 +0.7 1,890,461 + BWI76_RS08965 0.80 -0.3 1,890,469 - BWI76_RS08965 0.81 +0.5 1,890,474 + BWI76_RS08965 0.82 +0.4 1,890,487 - BWI76_RS08965 0.84 -1.6 1,890,645 - +0.1
Or see this region's nucleotide sequence