Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08815 and BWI76_RS08820 are separated by 11 nucleotides BWI76_RS08820 and BWI76_RS08825 are separated by 3 nucleotides BWI76_RS08825 and BWI76_RS08830 overlap by 8 nucleotides BWI76_RS08830 and BWI76_RS08835 are separated by 1 nucleotides
BWI76_RS08815: BWI76_RS08815 - cyclic pyranopterin phosphate synthase, at 1,858,509 to 1,859,508
_RS08815
BWI76_RS08820: BWI76_RS08820 - molybdenum cofactor biosynthesis protein, at 1,859,520 to 1,860,032
_RS08820
BWI76_RS08825: BWI76_RS08825 - cyclic pyranopterin monophosphate synthase MoaC, at 1,860,036 to 1,860,524
_RS08825
BWI76_RS08830: BWI76_RS08830 - molybdopterin synthase sulfur carrier subunit, at 1,860,517 to 1,860,765
_RS08830
BWI76_RS08835: BWI76_RS08835 - molybdopterin guanine dinucleotide biosynthesis protein MoaE, at 1,860,767 to 1,861,219
_RS08835
Position (kb) 1859
1860
1861 Strain fitness (log2 ratio) -1
0
1
2 at 1858.833 kb on + strand, within BWI76_RS08815 at 1859.001 kb on - strand, within BWI76_RS08815 at 1859.011 kb on + strand, within BWI76_RS08815 at 1859.062 kb on + strand, within BWI76_RS08815 at 1859.252 kb on + strand, within BWI76_RS08815 at 1859.327 kb on + strand, within BWI76_RS08815 at 1859.536 kb on - strand at 1859.592 kb on + strand, within BWI76_RS08820 at 1859.821 kb on - strand, within BWI76_RS08820 at 1859.866 kb on - strand, within BWI76_RS08820 at 1859.965 kb on + strand, within BWI76_RS08820 at 1860.154 kb on - strand, within BWI76_RS08825 at 1860.268 kb on - strand, within BWI76_RS08825 at 1860.425 kb on - strand, within BWI76_RS08825 at 1860.563 kb on - strand, within BWI76_RS08830 at 1860.768 kb on - strand at 1860.825 kb on - strand, within BWI76_RS08835 at 1860.886 kb on + strand, within BWI76_RS08835 at 1860.926 kb on - strand, within BWI76_RS08835 at 1860.928 kb on - strand, within BWI76_RS08835
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 1,858,833 + BWI76_RS08815 0.32 +0.3 1,859,001 - BWI76_RS08815 0.49 -0.6 1,859,011 + BWI76_RS08815 0.50 +0.8 1,859,062 + BWI76_RS08815 0.55 +0.3 1,859,252 + BWI76_RS08815 0.74 -0.5 1,859,327 + BWI76_RS08815 0.82 +0.1 1,859,536 - +1.3 1,859,592 + BWI76_RS08820 0.14 -0.7 1,859,821 - BWI76_RS08820 0.59 +0.9 1,859,866 - BWI76_RS08820 0.67 +1.1 1,859,965 + BWI76_RS08820 0.87 +1.3 1,860,154 - BWI76_RS08825 0.24 -0.1 1,860,268 - BWI76_RS08825 0.47 -0.0 1,860,425 - BWI76_RS08825 0.80 +2.4 1,860,563 - BWI76_RS08830 0.18 +0.3 1,860,768 - +0.3 1,860,825 - BWI76_RS08835 0.13 -0.0 1,860,886 + BWI76_RS08835 0.26 -0.3 1,860,926 - BWI76_RS08835 0.35 +0.1 1,860,928 - BWI76_RS08835 0.36 +0.5
Or see this region's nucleotide sequence