Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07775 and BWI76_RS07780 are separated by 55 nucleotides BWI76_RS07780 and BWI76_RS07785 overlap by 4 nucleotides BWI76_RS07785 and BWI76_RS07790 overlap by 20 nucleotides BWI76_RS07790 and BWI76_RS07795 are separated by 180 nucleotides
BWI76_RS07775: BWI76_RS07775 - carbon starvation protein A, at 1,648,525 to 1,650,630
_RS07775
BWI76_RS07780: BWI76_RS07780 - hypothetical protein, at 1,650,686 to 1,650,883
_RS07780
BWI76_RS07785: BWI76_RS07785 - transcriptional regulator, at 1,650,880 to 1,651,284
_RS07785
BWI76_RS07790: BWI76_RS07790 - plasmid stabilization protein, at 1,651,265 to 1,651,566
_RS07790
BWI76_RS07795: BWI76_RS07795 - oxidoreductase, at 1,651,747 to 1,652,835
_RS07795
Position (kb) 1650
1651 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1649.974 kb on - strand, within BWI76_RS07775 at 1650.134 kb on + strand, within BWI76_RS07775 at 1650.134 kb on + strand, within BWI76_RS07775 at 1650.242 kb on - strand, within BWI76_RS07775 at 1650.594 kb on - strand at 1650.633 kb on - strand at 1650.657 kb on - strand at 1650.773 kb on - strand, within BWI76_RS07780 at 1650.886 kb on - strand at 1650.966 kb on + strand, within BWI76_RS07785 at 1651.005 kb on + strand, within BWI76_RS07785 at 1651.288 kb on - strand at 1651.430 kb on - strand, within BWI76_RS07790 at 1651.827 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 1,649,974 - BWI76_RS07775 0.69 -0.2 1,650,134 + BWI76_RS07775 0.76 -0.1 1,650,134 + BWI76_RS07775 0.76 +2.3 1,650,242 - BWI76_RS07775 0.82 +0.5 1,650,594 - +0.3 1,650,633 - +1.1 1,650,657 - -0.4 1,650,773 - BWI76_RS07780 0.44 -2.0 1,650,886 - +0.3 1,650,966 + BWI76_RS07785 0.21 +1.3 1,651,005 + BWI76_RS07785 0.31 -2.3 1,651,288 - -0.3 1,651,430 - BWI76_RS07790 0.55 -0.4 1,651,827 + -2.9
Or see this region's nucleotide sequence