Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07555 and BWI76_RS07560 are separated by 108 nucleotides BWI76_RS07560 and BWI76_RS07565 are separated by 308 nucleotides BWI76_RS07565 and BWI76_RS07570 are separated by 24 nucleotides
BWI76_RS07555: BWI76_RS07555 - EamA family transporter, at 1,599,676 to 1,600,566
_RS07555
BWI76_RS07560: BWI76_RS07560 - aminotransferase regulator domain-containing protein, at 1,600,675 to 1,602,009
_RS07560
BWI76_RS07565: BWI76_RS07565 - LysE type translocator family protein, at 1,602,318 to 1,602,959
_RS07565
BWI76_RS07570: BWI76_RS07570 - GntR family transcriptional regulator with, at 1,602,984 to 1,604,450
_RS07570
Position (kb)
1600
1601
1602
1603 Strain fitness (log2 ratio)
-2
-1
0
1 at 1599.905 kb on + strand, within BWI76_RS07555 at 1599.910 kb on + strand, within BWI76_RS07555 at 1599.918 kb on - strand, within BWI76_RS07555 at 1600.059 kb on - strand at 1600.094 kb on + strand, within BWI76_RS07555 at 1600.130 kb on + strand, within BWI76_RS07555 at 1600.294 kb on - strand, within BWI76_RS07555 at 1600.516 kb on - strand at 1600.516 kb on - strand at 1600.618 kb on - strand at 1600.749 kb on - strand at 1600.771 kb on - strand at 1600.969 kb on + strand, within BWI76_RS07560 at 1600.982 kb on - strand, within BWI76_RS07560 at 1601.156 kb on + strand, within BWI76_RS07560 at 1601.225 kb on - strand, within BWI76_RS07560 at 1601.352 kb on - strand, within BWI76_RS07560 at 1601.471 kb on - strand, within BWI76_RS07560 at 1601.634 kb on + strand, within BWI76_RS07560 at 1601.634 kb on + strand, within BWI76_RS07560 at 1601.689 kb on + strand, within BWI76_RS07560 at 1601.805 kb on + strand, within BWI76_RS07560 at 1601.813 kb on - strand, within BWI76_RS07560 at 1601.941 kb on + strand at 1601.970 kb on - strand at 1602.055 kb on + strand at 1602.233 kb on + strand at 1602.290 kb on + strand at 1602.290 kb on + strand at 1602.298 kb on - strand at 1602.298 kb on - strand at 1602.308 kb on - strand at 1602.334 kb on + strand at 1602.395 kb on + strand, within BWI76_RS07565 at 1602.419 kb on + strand, within BWI76_RS07565 at 1602.445 kb on - strand, within BWI76_RS07565 at 1602.457 kb on + strand, within BWI76_RS07565 at 1602.463 kb on + strand, within BWI76_RS07565 at 1602.773 kb on + strand, within BWI76_RS07565 at 1602.773 kb on + strand, within BWI76_RS07565 at 1602.781 kb on - strand, within BWI76_RS07565 at 1602.802 kb on - strand, within BWI76_RS07565 at 1602.821 kb on - strand, within BWI76_RS07565 at 1602.835 kb on + strand, within BWI76_RS07565
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 1,599,905 + BWI76_RS07555 0.26 +0.3 1,599,910 + BWI76_RS07555 0.26 +0.2 1,599,918 - BWI76_RS07555 0.27 -0.3 1,600,059 - -1.1 1,600,094 + BWI76_RS07555 0.47 -2.2 1,600,130 + BWI76_RS07555 0.51 -0.6 1,600,294 - BWI76_RS07555 0.69 +0.1 1,600,516 - +0.8 1,600,516 - +0.3 1,600,618 - +1.2 1,600,749 - +0.4 1,600,771 - -0.3 1,600,969 + BWI76_RS07560 0.22 +0.3 1,600,982 - BWI76_RS07560 0.23 +0.6 1,601,156 + BWI76_RS07560 0.36 +0.8 1,601,225 - BWI76_RS07560 0.41 +0.6 1,601,352 - BWI76_RS07560 0.51 +1.1 1,601,471 - BWI76_RS07560 0.60 +0.1 1,601,634 + BWI76_RS07560 0.72 +0.2 1,601,634 + BWI76_RS07560 0.72 +1.2 1,601,689 + BWI76_RS07560 0.76 -1.6 1,601,805 + BWI76_RS07560 0.85 -0.1 1,601,813 - BWI76_RS07560 0.85 -0.0 1,601,941 + +0.1 1,601,970 - +0.5 1,602,055 + -1.3 1,602,233 + -1.8 1,602,290 + -0.5 1,602,290 + +1.2 1,602,298 - -0.2 1,602,298 - -0.3 1,602,308 - -1.6 1,602,334 + -0.7 1,602,395 + BWI76_RS07565 0.12 -0.7 1,602,419 + BWI76_RS07565 0.16 +0.2 1,602,445 - BWI76_RS07565 0.20 -0.3 1,602,457 + BWI76_RS07565 0.22 +0.8 1,602,463 + BWI76_RS07565 0.23 -0.3 1,602,773 + BWI76_RS07565 0.71 +0.5 1,602,773 + BWI76_RS07565 0.71 -0.3 1,602,781 - BWI76_RS07565 0.72 -1.1 1,602,802 - BWI76_RS07565 0.75 -2.4 1,602,821 - BWI76_RS07565 0.78 +0.4 1,602,835 + BWI76_RS07565 0.81 +0.4
Or see this region's nucleotide sequence