Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05440 and BWI76_RS05445 are separated by 32 nucleotides BWI76_RS05445 and BWI76_RS05450 overlap by 4 nucleotides BWI76_RS05450 and BWI76_RS05455 are separated by 65 nucleotides BWI76_RS05455 and BWI76_RS05460 are separated by 132 nucleotides
BWI76_RS05440: BWI76_RS05440 - hydroxyacylglutathione hydrolase, at 1,142,455 to 1,143,210
_RS05440
BWI76_RS05445: BWI76_RS05445 - type 11 methyltransferase, at 1,143,243 to 1,143,965
_RS05445
BWI76_RS05450: BWI76_RS05450 - ribonuclease HI, at 1,143,962 to 1,144,429
_RS05450
BWI76_RS05455: BWI76_RS05455 - DNA polymerase III subunit epsilon, at 1,144,495 to 1,145,226
_RS05455
BWI76_RS05460: BWI76_RS05460 - tRNA-Asp, at 1,145,359 to 1,145,435
_RS05460
Position (kb) 1143
1144
1145 Strain fitness (log2 ratio) -2
-1
0
1 at 1143.029 kb on + strand, within BWI76_RS05440 at 1143.076 kb on - strand, within BWI76_RS05440 at 1143.264 kb on - strand at 1143.294 kb on + strand at 1143.352 kb on - strand, within BWI76_RS05445 at 1143.352 kb on - strand, within BWI76_RS05445 at 1143.470 kb on + strand, within BWI76_RS05445 at 1143.470 kb on + strand, within BWI76_RS05445 at 1143.478 kb on - strand, within BWI76_RS05445 at 1143.478 kb on - strand, within BWI76_RS05445 at 1143.478 kb on - strand, within BWI76_RS05445 at 1143.478 kb on - strand, within BWI76_RS05445 at 1143.622 kb on + strand, within BWI76_RS05445 at 1143.641 kb on + strand, within BWI76_RS05445 at 1144.210 kb on + strand, within BWI76_RS05450 at 1144.317 kb on - strand, within BWI76_RS05450 at 1144.492 kb on + strand at 1145.308 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 1,143,029 + BWI76_RS05440 0.76 -0.4 1,143,076 - BWI76_RS05440 0.82 +1.0 1,143,264 - -0.3 1,143,294 + -1.5 1,143,352 - BWI76_RS05445 0.15 +0.1 1,143,352 - BWI76_RS05445 0.15 -0.9 1,143,470 + BWI76_RS05445 0.31 -0.5 1,143,470 + BWI76_RS05445 0.31 -0.4 1,143,478 - BWI76_RS05445 0.33 +1.5 1,143,478 - BWI76_RS05445 0.33 +0.3 1,143,478 - BWI76_RS05445 0.33 +1.2 1,143,478 - BWI76_RS05445 0.33 -2.1 1,143,622 + BWI76_RS05445 0.52 -0.6 1,143,641 + BWI76_RS05445 0.55 +0.5 1,144,210 + BWI76_RS05450 0.53 -1.6 1,144,317 - BWI76_RS05450 0.76 +1.2 1,144,492 + -1.0 1,145,308 + +1.6
Or see this region's nucleotide sequence