Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05015 and BWI76_RS05020 are separated by 164 nucleotides BWI76_RS05020 and BWI76_RS05025 overlap by 1 nucleotides BWI76_RS05025 and BWI76_RS05030 are separated by 72 nucleotides
BWI76_RS05015: BWI76_RS05015 - transcriptional regulator, at 1,051,627 to 1,052,082
_RS05015
BWI76_RS05020: BWI76_RS05020 - sugar fermentation stimulation protein SfsA, at 1,052,247 to 1,052,963
_RS05020
BWI76_RS05025: BWI76_RS05025 - 2'-5' RNA ligase, at 1,052,963 to 1,053,499
_RS05025
BWI76_RS05030: BWI76_RS05030 - ATP-dependent helicase, at 1,053,572 to 1,056,001
_RS05030
Position (kb)
1052
1053
1054 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1052.065 kb on + strand at 1052.187 kb on - strand at 1052.187 kb on - strand at 1052.187 kb on - strand at 1052.195 kb on - strand at 1052.195 kb on - strand at 1052.197 kb on - strand at 1052.264 kb on + strand at 1052.286 kb on - strand at 1052.291 kb on - strand at 1052.330 kb on + strand, within BWI76_RS05020 at 1052.381 kb on - strand, within BWI76_RS05020 at 1052.382 kb on + strand, within BWI76_RS05020 at 1052.390 kb on - strand, within BWI76_RS05020 at 1052.395 kb on + strand, within BWI76_RS05020 at 1052.395 kb on + strand, within BWI76_RS05020 at 1052.426 kb on - strand, within BWI76_RS05020 at 1052.524 kb on + strand, within BWI76_RS05020 at 1052.531 kb on - strand, within BWI76_RS05020 at 1052.543 kb on + strand, within BWI76_RS05020 at 1052.656 kb on + strand, within BWI76_RS05020 at 1052.779 kb on - strand, within BWI76_RS05020 at 1052.809 kb on - strand, within BWI76_RS05020 at 1052.823 kb on - strand, within BWI76_RS05020 at 1052.851 kb on + strand, within BWI76_RS05020 at 1052.860 kb on - strand, within BWI76_RS05020 at 1052.862 kb on + strand, within BWI76_RS05020 at 1052.871 kb on - strand, within BWI76_RS05020 at 1052.881 kb on - strand, within BWI76_RS05020 at 1053.280 kb on + strand, within BWI76_RS05025 at 1053.428 kb on + strand, within BWI76_RS05025 at 1053.463 kb on + strand at 1053.531 kb on + strand at 1053.840 kb on - strand, within BWI76_RS05030 at 1054.295 kb on - strand, within BWI76_RS05030 at 1054.333 kb on + strand, within BWI76_RS05030 at 1054.341 kb on - strand, within BWI76_RS05030 at 1054.440 kb on + strand, within BWI76_RS05030 at 1054.442 kb on + strand, within BWI76_RS05030
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 1,052,065 + -2.6 1,052,187 - -0.0 1,052,187 - -0.1 1,052,187 - +0.3 1,052,195 - +2.1 1,052,195 - -0.3 1,052,197 - -0.0 1,052,264 + +1.2 1,052,286 - +0.9 1,052,291 - -1.7 1,052,330 + BWI76_RS05020 0.12 -0.4 1,052,381 - BWI76_RS05020 0.19 -0.4 1,052,382 + BWI76_RS05020 0.19 +0.6 1,052,390 - BWI76_RS05020 0.20 +0.4 1,052,395 + BWI76_RS05020 0.21 -0.3 1,052,395 + BWI76_RS05020 0.21 -1.8 1,052,426 - BWI76_RS05020 0.25 -0.2 1,052,524 + BWI76_RS05020 0.39 +0.2 1,052,531 - BWI76_RS05020 0.40 -0.2 1,052,543 + BWI76_RS05020 0.41 +0.6 1,052,656 + BWI76_RS05020 0.57 +1.0 1,052,779 - BWI76_RS05020 0.74 +1.2 1,052,809 - BWI76_RS05020 0.78 -1.3 1,052,823 - BWI76_RS05020 0.80 -0.4 1,052,851 + BWI76_RS05020 0.84 +0.5 1,052,860 - BWI76_RS05020 0.85 +0.0 1,052,862 + BWI76_RS05020 0.86 +0.3 1,052,871 - BWI76_RS05020 0.87 -0.3 1,052,881 - BWI76_RS05020 0.88 -0.1 1,053,280 + BWI76_RS05025 0.59 -1.2 1,053,428 + BWI76_RS05025 0.87 -0.9 1,053,463 + +0.7 1,053,531 + +0.2 1,053,840 - BWI76_RS05030 0.11 +0.2 1,054,295 - BWI76_RS05030 0.30 +1.3 1,054,333 + BWI76_RS05030 0.31 +0.8 1,054,341 - BWI76_RS05030 0.32 +0.5 1,054,440 + BWI76_RS05030 0.36 +0.3 1,054,442 + BWI76_RS05030 0.36 +0.2
Or see this region's nucleotide sequence