Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05025

Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05015 and BWI76_RS05020 are separated by 164 nucleotidesBWI76_RS05020 and BWI76_RS05025 overlap by 1 nucleotidesBWI76_RS05025 and BWI76_RS05030 are separated by 72 nucleotides BWI76_RS05015: BWI76_RS05015 - transcriptional regulator, at 1,051,627 to 1,052,082 _RS05015 BWI76_RS05020: BWI76_RS05020 - sugar fermentation stimulation protein SfsA, at 1,052,247 to 1,052,963 _RS05020 BWI76_RS05025: BWI76_RS05025 - 2'-5' RNA ligase, at 1,052,963 to 1,053,499 _RS05025 BWI76_RS05030: BWI76_RS05030 - ATP-dependent helicase, at 1,053,572 to 1,056,001 _RS05030 Position (kb) 1052 1053 1054Strain fitness (log2 ratio) -2 -1 0 1 2at 1052.065 kb on + strandat 1052.187 kb on - strandat 1052.187 kb on - strandat 1052.187 kb on - strandat 1052.195 kb on - strandat 1052.195 kb on - strandat 1052.197 kb on - strandat 1052.264 kb on + strandat 1052.286 kb on - strandat 1052.291 kb on - strandat 1052.330 kb on + strand, within BWI76_RS05020at 1052.381 kb on - strand, within BWI76_RS05020at 1052.382 kb on + strand, within BWI76_RS05020at 1052.390 kb on - strand, within BWI76_RS05020at 1052.395 kb on + strand, within BWI76_RS05020at 1052.395 kb on + strand, within BWI76_RS05020at 1052.426 kb on - strand, within BWI76_RS05020at 1052.524 kb on + strand, within BWI76_RS05020at 1052.531 kb on - strand, within BWI76_RS05020at 1052.543 kb on + strand, within BWI76_RS05020at 1052.656 kb on + strand, within BWI76_RS05020at 1052.779 kb on - strand, within BWI76_RS05020at 1052.809 kb on - strand, within BWI76_RS05020at 1052.823 kb on - strand, within BWI76_RS05020at 1052.851 kb on + strand, within BWI76_RS05020at 1052.860 kb on - strand, within BWI76_RS05020at 1052.862 kb on + strand, within BWI76_RS05020at 1052.871 kb on - strand, within BWI76_RS05020at 1052.881 kb on - strand, within BWI76_RS05020at 1053.280 kb on + strand, within BWI76_RS05025at 1053.428 kb on + strand, within BWI76_RS05025at 1053.463 kb on + strandat 1053.531 kb on + strandat 1053.840 kb on - strand, within BWI76_RS05030at 1054.295 kb on - strand, within BWI76_RS05030at 1054.333 kb on + strand, within BWI76_RS05030at 1054.341 kb on - strand, within BWI76_RS05030at 1054.440 kb on + strand, within BWI76_RS05030at 1054.442 kb on + strand, within BWI76_RS05030

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
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1,052,065 + -2.6
1,052,187 - -0.0
1,052,187 - -0.1
1,052,187 - +0.3
1,052,195 - +2.1
1,052,195 - -0.3
1,052,197 - -0.0
1,052,264 + +1.2
1,052,286 - +0.9
1,052,291 - -1.7
1,052,330 + BWI76_RS05020 0.12 -0.4
1,052,381 - BWI76_RS05020 0.19 -0.4
1,052,382 + BWI76_RS05020 0.19 +0.6
1,052,390 - BWI76_RS05020 0.20 +0.4
1,052,395 + BWI76_RS05020 0.21 -0.3
1,052,395 + BWI76_RS05020 0.21 -1.8
1,052,426 - BWI76_RS05020 0.25 -0.2
1,052,524 + BWI76_RS05020 0.39 +0.2
1,052,531 - BWI76_RS05020 0.40 -0.2
1,052,543 + BWI76_RS05020 0.41 +0.6
1,052,656 + BWI76_RS05020 0.57 +1.0
1,052,779 - BWI76_RS05020 0.74 +1.2
1,052,809 - BWI76_RS05020 0.78 -1.3
1,052,823 - BWI76_RS05020 0.80 -0.4
1,052,851 + BWI76_RS05020 0.84 +0.5
1,052,860 - BWI76_RS05020 0.85 +0.0
1,052,862 + BWI76_RS05020 0.86 +0.3
1,052,871 - BWI76_RS05020 0.87 -0.3
1,052,881 - BWI76_RS05020 0.88 -0.1
1,053,280 + BWI76_RS05025 0.59 -1.2
1,053,428 + BWI76_RS05025 0.87 -0.9
1,053,463 + +0.7
1,053,531 + +0.2
1,053,840 - BWI76_RS05030 0.11 +0.2
1,054,295 - BWI76_RS05030 0.30 +1.3
1,054,333 + BWI76_RS05030 0.31 +0.8
1,054,341 - BWI76_RS05030 0.32 +0.5
1,054,440 + BWI76_RS05030 0.36 +0.3
1,054,442 + BWI76_RS05030 0.36 +0.2

Or see this region's nucleotide sequence