Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03285 and BWI76_RS03290 overlap by 35 nucleotides BWI76_RS03290 and BWI76_RS03295 are separated by 72 nucleotides BWI76_RS03295 and BWI76_RS03300 are separated by 32 nucleotides BWI76_RS03300 and BWI76_RS03305 overlap by 32 nucleotides
BWI76_RS03285: BWI76_RS03285 - hypothetical protein, at 678,853 to 679,071
_RS03285
BWI76_RS03290: BWI76_RS03290 - hypothetical protein, at 679,037 to 679,962
_RS03290
BWI76_RS03295: BWI76_RS03295 - transposase, at 680,035 to 680,298
_RS03295
BWI76_RS03300: BWI76_RS03300 - hypothetical protein, at 680,331 to 681,125
_RS03300
BWI76_RS03305: BWI76_RS03305 - hypothetical protein, at 681,094 to 682,334
_RS03305
Position (kb) 680
681 Strain fitness (log2 ratio) -2
-1
0
1
2 at 679.074 kb on - strand at 679.074 kb on - strand at 679.117 kb on + strand at 679.117 kb on + strand at 679.137 kb on - strand, within BWI76_RS03290 at 679.137 kb on - strand, within BWI76_RS03290 at 679.145 kb on + strand, within BWI76_RS03290 at 679.187 kb on - strand, within BWI76_RS03290 at 679.241 kb on - strand, within BWI76_RS03290 at 679.251 kb on + strand, within BWI76_RS03290 at 679.269 kb on + strand, within BWI76_RS03290 at 679.272 kb on - strand at 679.369 kb on + strand, within BWI76_RS03290 at 679.507 kb on + strand, within BWI76_RS03290 at 679.546 kb on + strand, within BWI76_RS03290 at 679.657 kb on + strand, within BWI76_RS03290 at 679.657 kb on + strand, within BWI76_RS03290 at 679.880 kb on + strand at 679.980 kb on + strand at 679.989 kb on + strand at 679.998 kb on - strand at 680.006 kb on + strand at 680.015 kb on + strand at 680.020 kb on + strand at 680.142 kb on + strand, within BWI76_RS03295 at 680.188 kb on - strand, within BWI76_RS03295 at 680.256 kb on + strand at 680.275 kb on - strand at 680.327 kb on + strand at 680.336 kb on - strand at 680.494 kb on + strand, within BWI76_RS03300 at 680.529 kb on + strand, within BWI76_RS03300 at 680.695 kb on + strand, within BWI76_RS03300 at 680.836 kb on - strand, within BWI76_RS03300 at 680.893 kb on + strand, within BWI76_RS03300 at 680.895 kb on + strand, within BWI76_RS03300 at 680.923 kb on + strand, within BWI76_RS03300
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 679,074 - +1.7 679,074 - -0.0 679,117 + +0.6 679,117 + -1.4 679,137 - BWI76_RS03290 0.11 +0.4 679,137 - BWI76_RS03290 0.11 +1.1 679,145 + BWI76_RS03290 0.12 -0.2 679,187 - BWI76_RS03290 0.16 -0.8 679,241 - BWI76_RS03290 0.22 +0.3 679,251 + BWI76_RS03290 0.23 -1.4 679,269 + BWI76_RS03290 0.25 +0.9 679,272 - +2.7 679,369 + BWI76_RS03290 0.36 -0.1 679,507 + BWI76_RS03290 0.51 +1.5 679,546 + BWI76_RS03290 0.55 +0.8 679,657 + BWI76_RS03290 0.67 +0.5 679,657 + BWI76_RS03290 0.67 -0.6 679,880 + -0.8 679,980 + -1.0 679,989 + -0.4 679,998 - -0.8 680,006 + -2.2 680,015 + -2.6 680,020 + -2.4 680,142 + BWI76_RS03295 0.41 -2.0 680,188 - BWI76_RS03295 0.58 -0.7 680,256 + +1.4 680,275 - +0.3 680,327 + +0.2 680,336 - -0.6 680,494 + BWI76_RS03300 0.21 +0.5 680,529 + BWI76_RS03300 0.25 -0.5 680,695 + BWI76_RS03300 0.46 -1.2 680,836 - BWI76_RS03300 0.64 -0.1 680,893 + BWI76_RS03300 0.71 -0.6 680,895 + BWI76_RS03300 0.71 +1.4 680,923 + BWI76_RS03300 0.74 -0.5
Or see this region's nucleotide sequence