Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02910 and BWI76_RS02915 are separated by 575 nucleotides BWI76_RS02915 and BWI76_RS02920 are separated by 18 nucleotides BWI76_RS02920 and BWI76_RS02925 are separated by 25 nucleotides
BWI76_RS02910: BWI76_RS02910 - hypothetical protein, at 594,761 to 595,153
_RS02910
BWI76_RS02915: BWI76_RS02915 - Hcp family T6SS protein Cts2H, at 595,729 to 596,214
_RS02915
BWI76_RS02920: BWI76_RS02920 - addiction module toxin RelE, at 596,233 to 596,379
_RS02920
BWI76_RS02925: BWI76_RS02925 - type IV secretion protein Rhs, at 596,405 to 598,123
_RS02925
Position (kb) 595
596
597 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 594.782 kb on - strand at 594.797 kb on + strand at 594.828 kb on + strand, within BWI76_RS02910 at 594.881 kb on + strand, within BWI76_RS02910 at 594.928 kb on + strand, within BWI76_RS02910 at 594.971 kb on - strand, within BWI76_RS02910 at 594.983 kb on - strand, within BWI76_RS02910 at 594.998 kb on - strand, within BWI76_RS02910 at 595.026 kb on + strand, within BWI76_RS02910 at 595.060 kb on + strand, within BWI76_RS02910 at 595.060 kb on + strand, within BWI76_RS02910 at 595.060 kb on + strand, within BWI76_RS02910 at 595.060 kb on + strand at 595.060 kb on + strand, within BWI76_RS02910 at 595.060 kb on + strand, within BWI76_RS02910 at 595.060 kb on + strand, within BWI76_RS02910 at 595.060 kb on + strand, within BWI76_RS02910 at 595.060 kb on + strand, within BWI76_RS02910 at 595.060 kb on + strand, within BWI76_RS02910 at 595.060 kb on + strand, within BWI76_RS02910 at 595.060 kb on + strand, within BWI76_RS02910 at 595.060 kb on + strand, within BWI76_RS02910 at 595.061 kb on + strand, within BWI76_RS02910 at 595.063 kb on - strand, within BWI76_RS02910 at 595.063 kb on - strand, within BWI76_RS02910 at 595.068 kb on - strand, within BWI76_RS02910 at 595.068 kb on - strand, within BWI76_RS02910 at 595.068 kb on - strand, within BWI76_RS02910 at 595.068 kb on - strand, within BWI76_RS02910 at 595.068 kb on - strand, within BWI76_RS02910 at 595.068 kb on - strand, within BWI76_RS02910 at 595.068 kb on - strand, within BWI76_RS02910 at 595.068 kb on - strand, within BWI76_RS02910 at 595.068 kb on - strand, within BWI76_RS02910 at 595.068 kb on - strand, within BWI76_RS02910 at 595.068 kb on - strand, within BWI76_RS02910 at 595.068 kb on - strand, within BWI76_RS02910 at 595.068 kb on - strand, within BWI76_RS02910 at 595.068 kb on - strand, within BWI76_RS02910 at 595.068 kb on - strand, within BWI76_RS02910 at 595.070 kb on + strand, within BWI76_RS02910 at 595.070 kb on + strand, within BWI76_RS02910 at 595.070 kb on + strand, within BWI76_RS02910 at 595.073 kb on - strand, within BWI76_RS02910 at 595.073 kb on - strand, within BWI76_RS02910 at 595.073 kb on - strand, within BWI76_RS02910 at 595.073 kb on - strand, within BWI76_RS02910 at 595.075 kb on + strand, within BWI76_RS02910 at 595.078 kb on - strand, within BWI76_RS02910 at 595.104 kb on - strand, within BWI76_RS02910 at 595.104 kb on - strand, within BWI76_RS02910 at 595.110 kb on - strand, within BWI76_RS02910 at 595.110 kb on - strand, within BWI76_RS02910 at 595.115 kb on + strand at 595.115 kb on - strand at 595.123 kb on - strand at 595.123 kb on - strand at 595.123 kb on - strand at 595.130 kb on + strand at 595.131 kb on + strand at 595.138 kb on + strand at 595.138 kb on + strand at 595.138 kb on + strand at 595.138 kb on + strand at 595.146 kb on - strand at 595.146 kb on - strand at 595.146 kb on - strand at 595.148 kb on + strand at 595.151 kb on + strand at 595.155 kb on + strand at 595.155 kb on + strand at 595.156 kb on - strand at 595.156 kb on - strand at 595.156 kb on - strand at 595.156 kb on - strand at 595.156 kb on - strand at 595.156 kb on - strand at 595.156 kb on - strand at 595.156 kb on - strand at 595.156 kb on - strand at 595.156 kb on - strand at 595.156 kb on - strand at 595.157 kb on - strand at 595.160 kb on + strand at 595.163 kb on - strand at 595.165 kb on + strand at 595.169 kb on - strand at 595.169 kb on - strand at 595.179 kb on - strand at 595.187 kb on - strand at 595.194 kb on - strand at 595.200 kb on + strand at 595.200 kb on + strand at 595.208 kb on - strand at 595.208 kb on - strand at 595.251 kb on - strand at 595.251 kb on - strand at 595.251 kb on - strand at 595.261 kb on - strand at 595.305 kb on + strand at 595.315 kb on + strand at 595.334 kb on + strand at 595.334 kb on - strand at 595.340 kb on - strand at 595.444 kb on + strand at 595.444 kb on + strand at 595.444 kb on + strand at 595.444 kb on + strand at 595.444 kb on + strand at 595.452 kb on - strand at 595.452 kb on - strand at 595.500 kb on + strand at 595.508 kb on - strand at 595.508 kb on - strand at 595.545 kb on + strand at 595.545 kb on + strand at 595.545 kb on + strand at 595.546 kb on + strand at 595.546 kb on + strand at 595.546 kb on + strand at 595.546 kb on + strand at 595.546 kb on + strand at 595.546 kb on + strand at 595.546 kb on + strand at 595.554 kb on - strand at 595.554 kb on - strand at 595.554 kb on - strand at 595.598 kb on - strand at 595.612 kb on - strand at 595.623 kb on + strand at 595.623 kb on + strand at 595.623 kb on + strand at 595.623 kb on + strand at 595.623 kb on + strand at 595.680 kb on + strand at 595.680 kb on + strand at 595.680 kb on + strand at 595.680 kb on + strand at 595.680 kb on + strand at 595.680 kb on + strand at 595.680 kb on + strand at 595.690 kb on + strand at 595.698 kb on - strand at 595.700 kb on + strand at 595.700 kb on + strand at 595.700 kb on + strand at 595.700 kb on + strand at 595.700 kb on + strand at 595.700 kb on + strand at 595.708 kb on - strand at 595.708 kb on - strand at 595.718 kb on - strand at 595.720 kb on + strand at 595.720 kb on + strand at 595.722 kb on + strand at 595.765 kb on + strand at 595.765 kb on + strand at 595.774 kb on - strand at 595.776 kb on + strand at 595.784 kb on - strand, within BWI76_RS02915 at 595.814 kb on + strand at 595.817 kb on + strand, within BWI76_RS02915 at 595.817 kb on + strand, within BWI76_RS02915 at 595.817 kb on + strand, within BWI76_RS02915 at 595.817 kb on + strand, within BWI76_RS02915 at 595.817 kb on + strand, within BWI76_RS02915 at 595.825 kb on - strand, within BWI76_RS02915 at 595.825 kb on - strand, within BWI76_RS02915 at 595.825 kb on - strand, within BWI76_RS02915 at 595.851 kb on + strand, within BWI76_RS02915 at 595.851 kb on + strand, within BWI76_RS02915 at 595.869 kb on - strand, within BWI76_RS02915 at 595.932 kb on + strand, within BWI76_RS02915 at 595.939 kb on + strand, within BWI76_RS02915 at 595.941 kb on + strand, within BWI76_RS02915 at 595.947 kb on - strand, within BWI76_RS02915 at 596.048 kb on + strand, within BWI76_RS02915 at 596.215 kb on + strand at 596.216 kb on + strand at 596.223 kb on - strand at 596.231 kb on - strand at 596.239 kb on - strand at 596.332 kb on + strand, within BWI76_RS02920 at 596.427 kb on + strand at 596.638 kb on + strand, within BWI76_RS02925 at 596.803 kb on - strand, within BWI76_RS02925 at 596.895 kb on + strand, within BWI76_RS02925 at 596.972 kb on + strand, within BWI76_RS02925 at 597.121 kb on + strand, within BWI76_RS02925 at 597.213 kb on + strand, within BWI76_RS02925
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 594,782 - -2.8 594,797 + -0.4 594,828 + BWI76_RS02910 0.17 +0.4 594,881 + BWI76_RS02910 0.31 -0.9 594,928 + BWI76_RS02910 0.42 +1.4 594,971 - BWI76_RS02910 0.53 +0.4 594,983 - BWI76_RS02910 0.56 -0.0 594,998 - BWI76_RS02910 0.60 -1.4 595,026 + BWI76_RS02910 0.67 -0.9 595,060 + BWI76_RS02910 0.76 -1.8 595,060 + BWI76_RS02910 0.76 +3.4 595,060 + BWI76_RS02910 0.76 -1.8 595,060 + +1.4 595,060 + BWI76_RS02910 0.76 -1.6 595,060 + BWI76_RS02910 0.76 -0.1 595,060 + BWI76_RS02910 0.76 -1.2 595,060 + BWI76_RS02910 0.76 -0.3 595,060 + BWI76_RS02910 0.76 +1.4 595,060 + BWI76_RS02910 0.76 +0.0 595,060 + BWI76_RS02910 0.76 +0.3 595,060 + BWI76_RS02910 0.76 +0.9 595,060 + BWI76_RS02910 0.76 -1.4 595,061 + BWI76_RS02910 0.76 -0.4 595,063 - BWI76_RS02910 0.77 +0.4 595,063 - BWI76_RS02910 0.77 -0.1 595,068 - BWI76_RS02910 0.78 -0.2 595,068 - BWI76_RS02910 0.78 +0.9 595,068 - BWI76_RS02910 0.78 -2.6 595,068 - BWI76_RS02910 0.78 -0.3 595,068 - BWI76_RS02910 0.78 +1.4 595,068 - BWI76_RS02910 0.78 +0.9 595,068 - BWI76_RS02910 0.78 +0.6 595,068 - BWI76_RS02910 0.78 +1.3 595,068 - BWI76_RS02910 0.78 +0.2 595,068 - BWI76_RS02910 0.78 -0.6 595,068 - BWI76_RS02910 0.78 -0.9 595,068 - BWI76_RS02910 0.78 +1.2 595,068 - BWI76_RS02910 0.78 +0.6 595,068 - BWI76_RS02910 0.78 -0.2 595,068 - BWI76_RS02910 0.78 -0.9 595,070 + BWI76_RS02910 0.79 +0.5 595,070 + BWI76_RS02910 0.79 -0.2 595,070 + BWI76_RS02910 0.79 -0.0 595,073 - BWI76_RS02910 0.79 +0.5 595,073 - BWI76_RS02910 0.79 +0.0 595,073 - BWI76_RS02910 0.79 +0.2 595,073 - BWI76_RS02910 0.79 -2.2 595,075 + BWI76_RS02910 0.80 -0.3 595,078 - BWI76_RS02910 0.81 +2.4 595,104 - BWI76_RS02910 0.87 +0.4 595,104 - BWI76_RS02910 0.87 +0.1 595,110 - BWI76_RS02910 0.89 -0.1 595,110 - BWI76_RS02910 0.89 -1.7 595,115 + -0.9 595,115 - +0.1 595,123 - -0.2 595,123 - +0.3 595,123 - -0.0 595,130 + +1.4 595,131 + +0.2 595,138 + +0.3 595,138 + -0.6 595,138 + -0.5 595,138 + -0.8 595,146 - +0.2 595,146 - +0.2 595,146 - -0.7 595,148 + -1.8 595,151 + -0.2 595,155 + -0.2 595,155 + -0.2 595,156 - +1.4 595,156 - +0.9 595,156 - +0.7 595,156 - -0.6 595,156 - +0.4 595,156 - +1.3 595,156 - +1.0 595,156 - -1.8 595,156 - +0.2 595,156 - -2.1 595,156 - -0.6 595,157 - +0.2 595,160 + +1.4 595,163 - -0.1 595,165 + -1.2 595,169 - -0.1 595,169 - -1.4 595,179 - -1.3 595,187 - -2.3 595,194 - -2.1 595,200 + +1.4 595,200 + -3.3 595,208 - -0.6 595,208 - -0.7 595,251 - +0.4 595,251 - -2.5 595,251 - -0.9 595,261 - +0.4 595,305 + +0.2 595,315 + -3.4 595,334 + +1.4 595,334 - -0.6 595,340 - -0.2 595,444 + +1.4 595,444 + +0.9 595,444 + +0.5 595,444 + +0.2 595,444 + +0.0 595,452 - +1.4 595,452 - +0.4 595,500 + +1.4 595,508 - +1.0 595,508 - +0.2 595,545 + -1.2 595,545 + +1.4 595,545 + -1.9 595,546 + +0.2 595,546 + +0.4 595,546 + +0.1 595,546 + -0.1 595,546 + -1.3 595,546 + -2.1 595,546 + -0.2 595,554 - -2.2 595,554 - -1.9 595,554 - +0.1 595,598 - -1.1 595,612 - +0.4 595,623 + +0.2 595,623 + +0.6 595,623 + +0.6 595,623 + -0.4 595,623 + +1.4 595,680 + +0.7 595,680 + -1.1 595,680 + -0.4 595,680 + -0.5 595,680 + -0.6 595,680 + -1.8 595,680 + -0.7 595,690 + +0.5 595,698 - -0.4 595,700 + -0.8 595,700 + -0.5 595,700 + -0.1 595,700 + +0.4 595,700 + -0.5 595,700 + +0.0 595,708 - -0.2 595,708 - -0.5 595,718 - -1.9 595,720 + +1.9 595,720 + +0.1 595,722 + +1.2 595,765 + -0.5 595,765 + -1.8 595,774 - -0.2 595,776 + +0.7 595,784 - BWI76_RS02915 0.11 -1.3 595,814 + +1.4 595,817 + BWI76_RS02915 0.18 -0.2 595,817 + BWI76_RS02915 0.18 +0.4 595,817 + BWI76_RS02915 0.18 -0.6 595,817 + BWI76_RS02915 0.18 +0.4 595,817 + BWI76_RS02915 0.18 -0.6 595,825 - BWI76_RS02915 0.20 -0.2 595,825 - BWI76_RS02915 0.20 +0.4 595,825 - BWI76_RS02915 0.20 +1.0 595,851 + BWI76_RS02915 0.25 -1.2 595,851 + BWI76_RS02915 0.25 -0.9 595,869 - BWI76_RS02915 0.29 -0.6 595,932 + BWI76_RS02915 0.42 +0.3 595,939 + BWI76_RS02915 0.43 -3.0 595,941 + BWI76_RS02915 0.44 -1.6 595,947 - BWI76_RS02915 0.45 -0.7 596,048 + BWI76_RS02915 0.66 +0.4 596,215 + +0.6 596,216 + +1.1 596,223 - -0.2 596,231 - +0.4 596,239 - +1.4 596,332 + BWI76_RS02920 0.67 -1.0 596,427 + +0.4 596,638 + BWI76_RS02925 0.14 +0.8 596,803 - BWI76_RS02925 0.23 -1.7 596,895 + BWI76_RS02925 0.29 -0.6 596,972 + BWI76_RS02925 0.33 -0.3 597,121 + BWI76_RS02925 0.42 -0.2 597,213 + BWI76_RS02925 0.47 -1.2
Or see this region's nucleotide sequence