Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02775 and BWI76_RS02780 overlap by 4 nucleotides BWI76_RS02780 and BWI76_RS02785 are separated by 2 nucleotides BWI76_RS02785 and BWI76_RS02790 are separated by 156 nucleotides
BWI76_RS02775: BWI76_RS02775 - outer membrane protein assembly factor, at 563,214 to 564,947
_RS02775
BWI76_RS02780: BWI76_RS02780 - translocation and assembly module TamB, at 564,944 to 568,720
_RS02780
BWI76_RS02785: BWI76_RS02785 - AIG2 family protein, at 568,723 to 569,067
_RS02785
BWI76_RS02790: BWI76_RS02790 - inorganic pyrophosphatase, at 569,224 to 569,751
_RS02790
Position (kb) 564
565
566
567
568
569 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 564.194 kb on - strand, within BWI76_RS02775 at 564.211 kb on + strand, within BWI76_RS02775 at 564.344 kb on - strand, within BWI76_RS02775 at 564.458 kb on + strand, within BWI76_RS02775 at 564.466 kb on + strand, within BWI76_RS02775 at 564.596 kb on + strand, within BWI76_RS02775 at 564.965 kb on + strand at 565.182 kb on + strand at 565.321 kb on + strand at 565.348 kb on - strand, within BWI76_RS02780 at 565.358 kb on - strand, within BWI76_RS02780 at 565.546 kb on + strand, within BWI76_RS02780 at 565.580 kb on - strand, within BWI76_RS02780 at 565.698 kb on + strand, within BWI76_RS02780 at 565.706 kb on - strand, within BWI76_RS02780 at 565.706 kb on - strand, within BWI76_RS02780 at 565.769 kb on + strand, within BWI76_RS02780 at 565.774 kb on + strand, within BWI76_RS02780 at 566.105 kb on - strand, within BWI76_RS02780 at 566.116 kb on - strand, within BWI76_RS02780 at 566.172 kb on - strand, within BWI76_RS02780 at 566.185 kb on + strand, within BWI76_RS02780 at 566.307 kb on + strand, within BWI76_RS02780 at 566.323 kb on - strand, within BWI76_RS02780 at 566.345 kb on - strand, within BWI76_RS02780 at 566.362 kb on - strand, within BWI76_RS02780 at 566.466 kb on + strand, within BWI76_RS02780 at 566.466 kb on + strand, within BWI76_RS02780 at 566.466 kb on + strand, within BWI76_RS02780 at 566.474 kb on - strand, within BWI76_RS02780 at 566.507 kb on + strand, within BWI76_RS02780 at 566.550 kb on + strand, within BWI76_RS02780 at 566.697 kb on + strand, within BWI76_RS02780 at 566.824 kb on + strand, within BWI76_RS02780 at 567.018 kb on - strand, within BWI76_RS02780 at 567.064 kb on + strand, within BWI76_RS02780 at 567.065 kb on - strand, within BWI76_RS02780 at 567.094 kb on - strand, within BWI76_RS02780 at 567.179 kb on + strand, within BWI76_RS02780 at 567.250 kb on + strand, within BWI76_RS02780 at 567.376 kb on - strand, within BWI76_RS02780 at 567.419 kb on + strand, within BWI76_RS02780 at 567.444 kb on + strand, within BWI76_RS02780 at 567.573 kb on - strand, within BWI76_RS02780 at 567.941 kb on + strand, within BWI76_RS02780 at 567.987 kb on + strand, within BWI76_RS02780 at 568.111 kb on + strand, within BWI76_RS02780 at 568.372 kb on + strand at 568.379 kb on - strand at 568.394 kb on + strand at 568.475 kb on - strand at 568.530 kb on + strand at 568.550 kb on + strand at 568.571 kb on - strand at 568.622 kb on + strand at 568.685 kb on - strand at 568.745 kb on + strand at 568.980 kb on - strand, within BWI76_RS02785 at 569.011 kb on + strand, within BWI76_RS02785 at 569.019 kb on - strand, within BWI76_RS02785
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 564,194 - BWI76_RS02775 0.57 -0.6 564,211 + BWI76_RS02775 0.57 +1.0 564,344 - BWI76_RS02775 0.65 -1.1 564,458 + BWI76_RS02775 0.72 -2.3 564,466 + BWI76_RS02775 0.72 -0.2 564,596 + BWI76_RS02775 0.80 -2.8 564,965 + -1.9 565,182 + -2.6 565,321 + -3.6 565,348 - BWI76_RS02780 0.11 -2.4 565,358 - BWI76_RS02780 0.11 -3.1 565,546 + BWI76_RS02780 0.16 -1.3 565,580 - BWI76_RS02780 0.17 -2.8 565,698 + BWI76_RS02780 0.20 -1.2 565,706 - BWI76_RS02780 0.20 -2.9 565,706 - BWI76_RS02780 0.20 -1.6 565,769 + BWI76_RS02780 0.22 -2.0 565,774 + BWI76_RS02780 0.22 -2.8 566,105 - BWI76_RS02780 0.31 -0.7 566,116 - BWI76_RS02780 0.31 -1.6 566,172 - BWI76_RS02780 0.33 -2.2 566,185 + BWI76_RS02780 0.33 -3.3 566,307 + BWI76_RS02780 0.36 -1.9 566,323 - BWI76_RS02780 0.37 -4.6 566,345 - BWI76_RS02780 0.37 -0.5 566,362 - BWI76_RS02780 0.38 -1.5 566,466 + BWI76_RS02780 0.40 -0.6 566,466 + BWI76_RS02780 0.40 -2.4 566,466 + BWI76_RS02780 0.40 -1.4 566,474 - BWI76_RS02780 0.41 -2.2 566,507 + BWI76_RS02780 0.41 -2.0 566,550 + BWI76_RS02780 0.43 -2.4 566,697 + BWI76_RS02780 0.46 -0.9 566,824 + BWI76_RS02780 0.50 -2.1 567,018 - BWI76_RS02780 0.55 -0.9 567,064 + BWI76_RS02780 0.56 -3.4 567,065 - BWI76_RS02780 0.56 -1.6 567,094 - BWI76_RS02780 0.57 -1.2 567,179 + BWI76_RS02780 0.59 -2.7 567,250 + BWI76_RS02780 0.61 -1.7 567,376 - BWI76_RS02780 0.64 -1.6 567,419 + BWI76_RS02780 0.66 -1.3 567,444 + BWI76_RS02780 0.66 -0.2 567,573 - BWI76_RS02780 0.70 -2.6 567,941 + BWI76_RS02780 0.79 -1.9 567,987 + BWI76_RS02780 0.81 +0.7 568,111 + BWI76_RS02780 0.84 -2.7 568,372 + -1.4 568,379 - -1.5 568,394 + -2.0 568,475 - +0.6 568,530 + +0.2 568,550 + -1.2 568,571 - -1.8 568,622 + -2.2 568,685 - -2.9 568,745 + -2.8 568,980 - BWI76_RS02785 0.74 +0.3 569,011 + BWI76_RS02785 0.83 +0.2 569,019 - BWI76_RS02785 0.86 -2.7
Or see this region's nucleotide sequence