Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02525 and BWI76_RS02530 overlap by 8 nucleotides BWI76_RS02530 and BWI76_RS02535 are separated by 92 nucleotides BWI76_RS02535 and BWI76_RS02540 are separated by 75 nucleotides
BWI76_RS02525: BWI76_RS02525 - DNA mismatch repair protein MutL, at 517,026 to 518,921
_RS02525
BWI76_RS02530: BWI76_RS02530 - tRNA dimethylallyltransferase, at 518,914 to 519,864
_RS02530
BWI76_RS02535: BWI76_RS02535 - RNA-binding protein Hfq, at 519,957 to 520,265
_RS02535
BWI76_RS02540: BWI76_RS02540 - GTPase HflX, at 520,341 to 521,621
_RS02540
Position (kb) 518
519
520 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 517.926 kb on - strand, within BWI76_RS02525 at 518.074 kb on - strand, within BWI76_RS02525 at 518.152 kb on + strand, within BWI76_RS02525 at 518.287 kb on + strand, within BWI76_RS02525 at 518.325 kb on + strand, within BWI76_RS02525 at 518.468 kb on - strand, within BWI76_RS02525 at 518.502 kb on + strand, within BWI76_RS02525 at 518.512 kb on + strand, within BWI76_RS02525 at 518.621 kb on - strand, within BWI76_RS02525 at 518.651 kb on + strand, within BWI76_RS02525 at 518.652 kb on - strand, within BWI76_RS02525 at 518.659 kb on - strand, within BWI76_RS02525 at 518.758 kb on + strand at 518.816 kb on + strand at 518.846 kb on + strand at 518.854 kb on - strand at 518.854 kb on - strand at 518.940 kb on + strand at 519.180 kb on + strand, within BWI76_RS02530 at 519.287 kb on - strand, within BWI76_RS02530 at 519.287 kb on - strand, within BWI76_RS02530 at 519.305 kb on - strand, within BWI76_RS02530 at 519.530 kb on + strand, within BWI76_RS02530 at 519.776 kb on + strand at 519.853 kb on + strand at 519.882 kb on + strand at 519.922 kb on + strand at 520.239 kb on + strand at 520.333 kb on + strand at 520.365 kb on - strand at 520.427 kb on - strand at 520.528 kb on - strand, within BWI76_RS02540 at 520.794 kb on - strand, within BWI76_RS02540 at 520.799 kb on - strand, within BWI76_RS02540 at 520.825 kb on - strand, within BWI76_RS02540 at 520.849 kb on + strand, within BWI76_RS02540
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 517,926 - BWI76_RS02525 0.47 -0.1 518,074 - BWI76_RS02525 0.55 -0.8 518,152 + BWI76_RS02525 0.59 -3.2 518,287 + BWI76_RS02525 0.67 +0.2 518,325 + BWI76_RS02525 0.69 -0.6 518,468 - BWI76_RS02525 0.76 +0.0 518,502 + BWI76_RS02525 0.78 -1.2 518,512 + BWI76_RS02525 0.78 +1.4 518,621 - BWI76_RS02525 0.84 -1.1 518,651 + BWI76_RS02525 0.86 -1.1 518,652 - BWI76_RS02525 0.86 -0.2 518,659 - BWI76_RS02525 0.86 -3.6 518,758 + -0.7 518,816 + +0.4 518,846 + -0.0 518,854 - +0.4 518,854 - -2.2 518,940 + -0.2 519,180 + BWI76_RS02530 0.28 -0.2 519,287 - BWI76_RS02530 0.39 +1.4 519,287 - BWI76_RS02530 0.39 +1.4 519,305 - BWI76_RS02530 0.41 -1.0 519,530 + BWI76_RS02530 0.65 -1.4 519,776 + -2.8 519,853 + +0.4 519,882 + +0.3 519,922 + +1.3 520,239 + -1.2 520,333 + +1.4 520,365 - +1.4 520,427 - -4.4 520,528 - BWI76_RS02540 0.15 +1.4 520,794 - BWI76_RS02540 0.35 -1.2 520,799 - BWI76_RS02540 0.36 -1.3 520,825 - BWI76_RS02540 0.38 -0.8 520,849 + BWI76_RS02540 0.40 +0.5
Or see this region's nucleotide sequence