Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00390 and BWI76_RS00395 overlap by 49 nucleotides BWI76_RS00395 and BWI76_RS00400 are separated by 57 nucleotides BWI76_RS00400 and BWI76_RS00405 are separated by 215 nucleotides BWI76_RS00405 and BWI76_RS00410 overlap by 48 nucleotides
BWI76_RS00390: BWI76_RS00390 - two-component system sensor histidine kinase NtrB, at 82,296 to 83,345
_RS00390
BWI76_RS00395: BWI76_RS00395 - hypothetical protein, at 83,297 to 83,476
_RS00395
BWI76_RS00400: BWI76_RS00400 - glutamine synthetase, at 83,534 to 84,943
_RS00400
BWI76_RS00405: BWI76_RS00405 - hypothetical protein, at 85,159 to 85,369
_RS00405
BWI76_RS00410: BWI76_RS00410 - translational GTPase TypA, at 85,322 to 87,145
_RS00410
Position (kb) 83
84
85 Strain fitness (log2 ratio) -2
-1
0
1 at 82.861 kb on + strand, within BWI76_RS00390 at 82.889 kb on + strand, within BWI76_RS00390 at 83.033 kb on + strand, within BWI76_RS00390 at 83.040 kb on + strand, within BWI76_RS00390 at 83.142 kb on + strand, within BWI76_RS00390 at 83.186 kb on - strand, within BWI76_RS00390 at 83.302 kb on + strand at 83.501 kb on - strand at 83.508 kb on - strand at 83.541 kb on + strand at 83.636 kb on + strand at 83.695 kb on + strand, within BWI76_RS00400 at 83.819 kb on - strand, within BWI76_RS00400 at 84.616 kb on + strand, within BWI76_RS00400 at 84.953 kb on + strand at 84.961 kb on - strand at 85.041 kb on - strand at 85.289 kb on - strand, within BWI76_RS00405
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 82,861 + BWI76_RS00390 0.54 -0.2 82,889 + BWI76_RS00390 0.56 +1.3 83,033 + BWI76_RS00390 0.70 -0.7 83,040 + BWI76_RS00390 0.71 +0.3 83,142 + BWI76_RS00390 0.81 +0.4 83,186 - BWI76_RS00390 0.85 +0.5 83,302 + -0.1 83,501 - -1.5 83,508 - -0.6 83,541 + +1.0 83,636 + +1.3 83,695 + BWI76_RS00400 0.11 +0.3 83,819 - BWI76_RS00400 0.20 +1.3 84,616 + BWI76_RS00400 0.77 -0.7 84,953 + -1.3 84,961 - -1.1 85,041 - -1.8 85,289 - BWI76_RS00405 0.62 -0.7
Or see this region's nucleotide sequence