Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00090 and BWI76_RS00095 overlap by 32 nucleotides BWI76_RS00095 and BWI76_RS00100 are separated by 147 nucleotides BWI76_RS00100 and BWI76_RS00105 are separated by 37 nucleotides
BWI76_RS00090: BWI76_RS00090 - multidrug transporter subunit MdtL, at 17,212 to 18,393
_RS00090
BWI76_RS00095: BWI76_RS00095 - DNA-binding transcriptional regulator, at 18,362 to 19,321
_RS00095
BWI76_RS00100: BWI76_RS00100 - hypothetical protein, at 19,469 to 20,206
_RS00100
BWI76_RS00105: BWI76_RS00105 - NAD(P)H-dependent oxidoreductase, at 20,244 to 20,810
_RS00105
Position (kb) 18
19
20 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 17.498 kb on - strand, within BWI76_RS00090 at 17.539 kb on + strand, within BWI76_RS00090 at 17.546 kb on + strand, within BWI76_RS00090 at 17.672 kb on + strand, within BWI76_RS00090 at 17.683 kb on + strand, within BWI76_RS00090 at 17.688 kb on + strand, within BWI76_RS00090 at 17.696 kb on - strand, within BWI76_RS00090 at 17.881 kb on - strand, within BWI76_RS00090 at 17.963 kb on + strand, within BWI76_RS00090 at 18.025 kb on - strand, within BWI76_RS00090 at 18.069 kb on - strand, within BWI76_RS00090 at 18.111 kb on + strand, within BWI76_RS00090 at 18.167 kb on + strand, within BWI76_RS00090 at 18.217 kb on - strand, within BWI76_RS00090 at 18.219 kb on - strand, within BWI76_RS00090 at 18.343 kb on - strand at 18.381 kb on + strand at 18.544 kb on - strand at 18.570 kb on + strand, within BWI76_RS00095 at 18.904 kb on + strand, within BWI76_RS00095 at 19.096 kb on + strand, within BWI76_RS00095 at 19.227 kb on + strand at 19.300 kb on + strand at 19.308 kb on - strand at 19.308 kb on - strand at 19.313 kb on - strand at 19.623 kb on - strand, within BWI76_RS00100 at 19.625 kb on - strand, within BWI76_RS00100 at 19.967 kb on + strand, within BWI76_RS00100 at 20.030 kb on + strand, within BWI76_RS00100 at 20.038 kb on - strand, within BWI76_RS00100 at 20.053 kb on - strand, within BWI76_RS00100 at 20.123 kb on + strand, within BWI76_RS00100 at 20.244 kb on - strand at 20.281 kb on - strand at 20.314 kb on + strand, within BWI76_RS00105
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 17,498 - BWI76_RS00090 0.24 +0.1 17,539 + BWI76_RS00090 0.28 -1.4 17,546 + BWI76_RS00090 0.28 +0.7 17,672 + BWI76_RS00090 0.39 -0.7 17,683 + BWI76_RS00090 0.40 +0.1 17,688 + BWI76_RS00090 0.40 +0.2 17,696 - BWI76_RS00090 0.41 -0.1 17,881 - BWI76_RS00090 0.57 +0.7 17,963 + BWI76_RS00090 0.64 +0.6 18,025 - BWI76_RS00090 0.69 +0.1 18,069 - BWI76_RS00090 0.73 -1.3 18,111 + BWI76_RS00090 0.76 +1.0 18,167 + BWI76_RS00090 0.81 +1.0 18,217 - BWI76_RS00090 0.85 -0.4 18,219 - BWI76_RS00090 0.85 +0.1 18,343 - -1.0 18,381 + +0.2 18,544 - -0.3 18,570 + BWI76_RS00095 0.22 -0.8 18,904 + BWI76_RS00095 0.56 -3.1 19,096 + BWI76_RS00095 0.76 -0.3 19,227 + -1.7 19,300 + -0.2 19,308 - +1.3 19,308 - +0.4 19,313 - -0.8 19,623 - BWI76_RS00100 0.21 +0.4 19,625 - BWI76_RS00100 0.21 +0.6 19,967 + BWI76_RS00100 0.67 -0.3 20,030 + BWI76_RS00100 0.76 +0.0 20,038 - BWI76_RS00100 0.77 +0.9 20,053 - BWI76_RS00100 0.79 -1.0 20,123 + BWI76_RS00100 0.89 -1.0 20,244 - +0.4 20,281 - -0.3 20,314 + BWI76_RS00105 0.12 -0.9
Or see this region's nucleotide sequence