Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26350 and BWI76_RS26355 are separated by 153 nucleotides BWI76_RS26355 and BWI76_RS26360 are separated by 31 nucleotides BWI76_RS26360 and BWI76_RS26365 are separated by 248 nucleotides
BWI76_RS26350: BWI76_RS26350 - branched chain amino acid ABC transporter substrate-binding protein, at 5,454,357 to 5,455,466
_RS26350
BWI76_RS26355: BWI76_RS26355 - hypothetical protein, at 5,455,620 to 5,455,855
_RS26355
BWI76_RS26360: BWI76_RS26360 - aspartate 1-decarboxylase autocleavage activator PanM, at 5,455,887 to 5,456,273
_RS26360
BWI76_RS26365: BWI76_RS26365 - branched chain amino acid ABC transporter substrate-binding protein, at 5,456,522 to 5,457,640
_RS26365
Position (kb) 5455
5456
5457 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5454.926 kb on - strand, within BWI76_RS26350 at 5455.090 kb on - strand, within BWI76_RS26350 at 5455.170 kb on - strand, within BWI76_RS26350 at 5455.229 kb on + strand, within BWI76_RS26350 at 5455.248 kb on + strand, within BWI76_RS26350 at 5455.349 kb on + strand, within BWI76_RS26350 at 5455.492 kb on - strand at 5455.552 kb on + strand at 5455.770 kb on + strand, within BWI76_RS26355 at 5455.770 kb on + strand, within BWI76_RS26355 at 5455.775 kb on + strand, within BWI76_RS26355 at 5455.778 kb on - strand, within BWI76_RS26355 at 5455.783 kb on - strand, within BWI76_RS26355 at 5455.924 kb on - strand at 5455.924 kb on - strand at 5456.600 kb on - strand at 5456.689 kb on - strand, within BWI76_RS26365 at 5456.710 kb on + strand, within BWI76_RS26365 at 5456.850 kb on + strand, within BWI76_RS26365 at 5456.858 kb on - strand, within BWI76_RS26365 at 5457.085 kb on - strand, within BWI76_RS26365 at 5457.092 kb on + strand, within BWI76_RS26365 at 5457.105 kb on + strand, within BWI76_RS26365 at 5457.262 kb on + strand, within BWI76_RS26365
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 5,454,926 - BWI76_RS26350 0.51 -0.1 5,455,090 - BWI76_RS26350 0.66 +1.4 5,455,170 - BWI76_RS26350 0.73 -2.3 5,455,229 + BWI76_RS26350 0.79 +1.7 5,455,248 + BWI76_RS26350 0.80 +0.8 5,455,349 + BWI76_RS26350 0.89 +1.2 5,455,492 - +1.8 5,455,552 + +0.9 5,455,770 + BWI76_RS26355 0.64 +0.7 5,455,770 + BWI76_RS26355 0.64 +1.6 5,455,775 + BWI76_RS26355 0.66 +0.8 5,455,778 - BWI76_RS26355 0.67 -0.3 5,455,783 - BWI76_RS26355 0.69 +0.3 5,455,924 - +0.1 5,455,924 - -1.2 5,456,600 - +1.4 5,456,689 - BWI76_RS26365 0.15 -0.2 5,456,710 + BWI76_RS26365 0.17 -0.5 5,456,850 + BWI76_RS26365 0.29 +0.3 5,456,858 - BWI76_RS26365 0.30 +0.5 5,457,085 - BWI76_RS26365 0.50 -0.8 5,457,092 + BWI76_RS26365 0.51 +1.4 5,457,105 + BWI76_RS26365 0.52 +1.5 5,457,262 + BWI76_RS26365 0.66 -1.5
Or see this region's nucleotide sequence