Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24245 and BWI76_RS24250 are separated by 204 nucleotides BWI76_RS24250 and BWI76_RS24255 are separated by 60 nucleotides BWI76_RS24255 and BWI76_RS24260 are separated by 35 nucleotides
BWI76_RS24245: BWI76_RS24245 - bifunctional glutathionylspermidine amidase/glutathionylspermidine synthase, at 5,052,296 to 5,054,167
_RS24245
BWI76_RS24250: BWI76_RS24250 - hypothetical protein, at 5,054,372 to 5,054,932
_RS24250
BWI76_RS24255: BWI76_RS24255 - thiol:disulfide oxidoreductase, at 5,054,993 to 5,055,859
_RS24255
BWI76_RS24260: BWI76_RS24260 - hypothetical protein, at 5,055,895 to 5,056,182
_RS24260
Position (kb) 5054
5055 Strain fitness (log2 ratio) -2
-1
0
1 at 5053.497 kb on + strand, within BWI76_RS24245 at 5053.673 kb on - strand, within BWI76_RS24245 at 5053.819 kb on - strand, within BWI76_RS24245 at 5053.824 kb on - strand, within BWI76_RS24245 at 5054.009 kb on + strand at 5054.117 kb on + strand at 5054.117 kb on + strand at 5054.125 kb on - strand at 5054.125 kb on - strand at 5054.274 kb on - strand at 5054.407 kb on + strand at 5054.415 kb on - strand at 5054.438 kb on - strand, within BWI76_RS24250 at 5054.452 kb on + strand, within BWI76_RS24250 at 5054.485 kb on + strand, within BWI76_RS24250 at 5054.636 kb on - strand, within BWI76_RS24250 at 5054.676 kb on + strand, within BWI76_RS24250 at 5055.025 kb on - strand at 5055.447 kb on + strand, within BWI76_RS24255 at 5055.549 kb on + strand, within BWI76_RS24255 at 5055.751 kb on - strand, within BWI76_RS24255 at 5055.774 kb on + strand at 5055.918 kb on + strand at 5055.926 kb on - strand, within BWI76_RS24260
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 5,053,497 + BWI76_RS24245 0.64 -0.0 5,053,673 - BWI76_RS24245 0.74 -2.1 5,053,819 - BWI76_RS24245 0.81 -2.5 5,053,824 - BWI76_RS24245 0.82 -1.0 5,054,009 + +1.3 5,054,117 + +1.3 5,054,117 + -0.4 5,054,125 - +0.3 5,054,125 - -0.5 5,054,274 - +0.3 5,054,407 + -2.2 5,054,415 - -0.7 5,054,438 - BWI76_RS24250 0.12 -0.4 5,054,452 + BWI76_RS24250 0.14 -0.4 5,054,485 + BWI76_RS24250 0.20 +0.6 5,054,636 - BWI76_RS24250 0.47 -0.5 5,054,676 + BWI76_RS24250 0.54 +1.4 5,055,025 - +0.4 5,055,447 + BWI76_RS24255 0.52 +0.7 5,055,549 + BWI76_RS24255 0.64 +0.3 5,055,751 - BWI76_RS24255 0.87 +1.3 5,055,774 + -0.7 5,055,918 + +0.5 5,055,926 - BWI76_RS24260 0.11 +1.5
Or see this region's nucleotide sequence