Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24175 and BWI76_RS24180 are separated by 70 nucleotides BWI76_RS24180 and BWI76_RS24185 are separated by 168 nucleotides BWI76_RS24185 and BWI76_RS24190 are separated by 105 nucleotides BWI76_RS24190 and BWI76_RS24195 are separated by 135 nucleotides
BWI76_RS24175: BWI76_RS24175 - ornithine decarboxylase, at 5,039,952 to 5,042,090
_RS24175
BWI76_RS24180: BWI76_RS24180 - hypothetical protein, at 5,042,161 to 5,042,359
_RS24180
BWI76_RS24185: BWI76_RS24185 - membrane protein, at 5,042,528 to 5,043,244
_RS24185
BWI76_RS24190: BWI76_RS24190 - tRNA-Phe, at 5,043,350 to 5,043,425
_RS24190
BWI76_RS24195: BWI76_RS24195 - anion transporter, at 5,043,561 to 5,044,949
_RS24195
Position (kb) 5042
5043
5044 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 5041.580 kb on - strand, within BWI76_RS24175 at 5041.638 kb on - strand, within BWI76_RS24175 at 5041.690 kb on + strand, within BWI76_RS24175 at 5041.839 kb on + strand, within BWI76_RS24175 at 5041.920 kb on + strand at 5042.011 kb on - strand at 5042.013 kb on + strand at 5042.013 kb on + strand at 5042.013 kb on + strand at 5042.021 kb on - strand at 5042.021 kb on - strand at 5042.036 kb on + strand at 5042.057 kb on - strand at 5042.138 kb on + strand at 5042.141 kb on + strand at 5042.160 kb on + strand at 5042.249 kb on + strand, within BWI76_RS24180 at 5042.257 kb on - strand, within BWI76_RS24180 at 5042.372 kb on + strand at 5042.407 kb on + strand at 5042.407 kb on + strand at 5042.528 kb on + strand at 5042.692 kb on + strand, within BWI76_RS24185 at 5042.722 kb on - strand, within BWI76_RS24185 at 5042.773 kb on + strand, within BWI76_RS24185 at 5042.781 kb on - strand, within BWI76_RS24185 at 5042.843 kb on + strand, within BWI76_RS24185 at 5042.999 kb on - strand, within BWI76_RS24185 at 5043.130 kb on - strand, within BWI76_RS24185 at 5043.138 kb on - strand, within BWI76_RS24185 at 5043.514 kb on - strand at 5043.514 kb on - strand at 5043.519 kb on - strand at 5043.539 kb on - strand at 5043.612 kb on + strand at 5043.613 kb on + strand at 5043.623 kb on + strand at 5043.633 kb on + strand at 5043.636 kb on - strand at 5043.639 kb on - strand at 5043.645 kb on - strand at 5043.684 kb on - strand at 5043.691 kb on + strand at 5043.691 kb on + strand at 5043.699 kb on - strand at 5043.701 kb on + strand, within BWI76_RS24195 at 5043.701 kb on + strand, within BWI76_RS24195 at 5043.701 kb on + strand, within BWI76_RS24195 at 5043.701 kb on + strand, within BWI76_RS24195 at 5043.701 kb on + strand, within BWI76_RS24195 at 5043.701 kb on + strand, within BWI76_RS24195 at 5043.701 kb on + strand, within BWI76_RS24195 at 5043.701 kb on + strand at 5043.709 kb on - strand, within BWI76_RS24195 at 5043.709 kb on - strand, within BWI76_RS24195 at 5043.709 kb on - strand, within BWI76_RS24195 at 5043.709 kb on - strand, within BWI76_RS24195 at 5043.709 kb on - strand, within BWI76_RS24195 at 5043.709 kb on - strand, within BWI76_RS24195 at 5043.709 kb on - strand, within BWI76_RS24195 at 5043.709 kb on - strand, within BWI76_RS24195 at 5043.709 kb on - strand, within BWI76_RS24195 at 5043.709 kb on - strand, within BWI76_RS24195 at 5043.709 kb on - strand, within BWI76_RS24195 at 5043.709 kb on - strand, within BWI76_RS24195 at 5043.709 kb on - strand, within BWI76_RS24195 at 5043.743 kb on + strand, within BWI76_RS24195 at 5043.748 kb on + strand, within BWI76_RS24195 at 5043.764 kb on - strand, within BWI76_RS24195 at 5043.773 kb on - strand, within BWI76_RS24195 at 5043.774 kb on - strand, within BWI76_RS24195 at 5043.790 kb on + strand, within BWI76_RS24195 at 5043.795 kb on + strand, within BWI76_RS24195 at 5043.800 kb on + strand, within BWI76_RS24195 at 5043.800 kb on + strand, within BWI76_RS24195 at 5043.800 kb on + strand, within BWI76_RS24195 at 5043.803 kb on - strand at 5043.803 kb on - strand, within BWI76_RS24195 at 5043.803 kb on - strand, within BWI76_RS24195 at 5043.803 kb on - strand, within BWI76_RS24195 at 5043.805 kb on + strand, within BWI76_RS24195 at 5043.808 kb on - strand, within BWI76_RS24195 at 5043.813 kb on - strand, within BWI76_RS24195 at 5043.848 kb on - strand, within BWI76_RS24195 at 5043.893 kb on + strand, within BWI76_RS24195 at 5043.901 kb on - strand, within BWI76_RS24195 at 5043.903 kb on + strand, within BWI76_RS24195 at 5043.903 kb on + strand, within BWI76_RS24195 at 5043.906 kb on - strand, within BWI76_RS24195 at 5043.908 kb on + strand, within BWI76_RS24195 at 5043.908 kb on + strand at 5043.913 kb on + strand, within BWI76_RS24195 at 5043.916 kb on - strand, within BWI76_RS24195 at 5043.918 kb on + strand, within BWI76_RS24195 at 5043.918 kb on + strand, within BWI76_RS24195 at 5043.924 kb on + strand, within BWI76_RS24195 at 5043.926 kb on - strand, within BWI76_RS24195 at 5043.931 kb on - strand, within BWI76_RS24195 at 5043.933 kb on + strand, within BWI76_RS24195 at 5043.948 kb on - strand, within BWI76_RS24195 at 5043.992 kb on + strand, within BWI76_RS24195 at 5044.005 kb on + strand, within BWI76_RS24195 at 5044.013 kb on - strand at 5044.013 kb on - strand at 5044.020 kb on - strand at 5044.036 kb on + strand, within BWI76_RS24195 at 5044.054 kb on - strand, within BWI76_RS24195 at 5044.054 kb on - strand, within BWI76_RS24195 at 5044.063 kb on + strand, within BWI76_RS24195 at 5044.072 kb on + strand, within BWI76_RS24195 at 5044.082 kb on + strand, within BWI76_RS24195 at 5044.082 kb on + strand, within BWI76_RS24195 at 5044.082 kb on + strand, within BWI76_RS24195 at 5044.090 kb on - strand, within BWI76_RS24195 at 5044.090 kb on - strand, within BWI76_RS24195 at 5044.097 kb on + strand, within BWI76_RS24195 at 5044.098 kb on - strand, within BWI76_RS24195 at 5044.110 kb on - strand, within BWI76_RS24195 at 5044.110 kb on - strand, within BWI76_RS24195 at 5044.110 kb on - strand, within BWI76_RS24195 at 5044.112 kb on - strand, within BWI76_RS24195 at 5044.131 kb on - strand, within BWI76_RS24195 at 5044.147 kb on + strand, within BWI76_RS24195 at 5044.147 kb on + strand at 5044.170 kb on + strand, within BWI76_RS24195 at 5044.173 kb on - strand, within BWI76_RS24195 at 5044.190 kb on + strand, within BWI76_RS24195 at 5044.210 kb on - strand, within BWI76_RS24195 at 5044.213 kb on + strand, within BWI76_RS24195 at 5044.220 kb on + strand, within BWI76_RS24195 at 5044.220 kb on - strand, within BWI76_RS24195 at 5044.228 kb on - strand, within BWI76_RS24195 at 5044.228 kb on - strand, within BWI76_RS24195
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 5,041,580 - BWI76_RS24175 0.76 +0.2 5,041,638 - BWI76_RS24175 0.79 +0.3 5,041,690 + BWI76_RS24175 0.81 -2.0 5,041,839 + BWI76_RS24175 0.88 -0.5 5,041,920 + -2.4 5,042,011 - +0.7 5,042,013 + -0.8 5,042,013 + +0.3 5,042,013 + +1.8 5,042,021 - +1.1 5,042,021 - +1.3 5,042,036 + +0.2 5,042,057 - -1.1 5,042,138 + +1.2 5,042,141 + +2.2 5,042,160 + +0.2 5,042,249 + BWI76_RS24180 0.44 -2.6 5,042,257 - BWI76_RS24180 0.48 +0.8 5,042,372 + +1.2 5,042,407 + +0.0 5,042,407 + +0.3 5,042,528 + +0.9 5,042,692 + BWI76_RS24185 0.23 -0.2 5,042,722 - BWI76_RS24185 0.27 -3.9 5,042,773 + BWI76_RS24185 0.34 -0.9 5,042,781 - BWI76_RS24185 0.35 -1.4 5,042,843 + BWI76_RS24185 0.44 -0.2 5,042,999 - BWI76_RS24185 0.66 +0.0 5,043,130 - BWI76_RS24185 0.84 -0.6 5,043,138 - BWI76_RS24185 0.85 +0.3 5,043,514 - -2.1 5,043,514 - -0.1 5,043,519 - -2.5 5,043,539 - -1.2 5,043,612 + +0.0 5,043,613 + -2.0 5,043,623 + -0.4 5,043,633 + -1.1 5,043,636 - -1.8 5,043,639 - +0.7 5,043,645 - +1.6 5,043,684 - +0.6 5,043,691 + -0.2 5,043,691 + -0.7 5,043,699 - -1.2 5,043,701 + BWI76_RS24195 0.10 +0.0 5,043,701 + BWI76_RS24195 0.10 -2.7 5,043,701 + BWI76_RS24195 0.10 -1.4 5,043,701 + BWI76_RS24195 0.10 +0.6 5,043,701 + BWI76_RS24195 0.10 -0.2 5,043,701 + BWI76_RS24195 0.10 -2.0 5,043,701 + BWI76_RS24195 0.10 -3.7 5,043,701 + +1.7 5,043,709 - BWI76_RS24195 0.11 -0.2 5,043,709 - BWI76_RS24195 0.11 +0.8 5,043,709 - BWI76_RS24195 0.11 +0.6 5,043,709 - BWI76_RS24195 0.11 +1.0 5,043,709 - BWI76_RS24195 0.11 -0.4 5,043,709 - BWI76_RS24195 0.11 -1.4 5,043,709 - BWI76_RS24195 0.11 -1.2 5,043,709 - BWI76_RS24195 0.11 -0.0 5,043,709 - BWI76_RS24195 0.11 -1.8 5,043,709 - BWI76_RS24195 0.11 -1.7 5,043,709 - BWI76_RS24195 0.11 -2.1 5,043,709 - BWI76_RS24195 0.11 -1.5 5,043,709 - BWI76_RS24195 0.11 -0.7 5,043,743 + BWI76_RS24195 0.13 -1.0 5,043,748 + BWI76_RS24195 0.13 -0.5 5,043,764 - BWI76_RS24195 0.15 +1.3 5,043,773 - BWI76_RS24195 0.15 +1.3 5,043,774 - BWI76_RS24195 0.15 -0.8 5,043,790 + BWI76_RS24195 0.16 -2.1 5,043,795 + BWI76_RS24195 0.17 -0.3 5,043,800 + BWI76_RS24195 0.17 +2.1 5,043,800 + BWI76_RS24195 0.17 +1.6 5,043,800 + BWI76_RS24195 0.17 +0.3 5,043,803 - +1.3 5,043,803 - BWI76_RS24195 0.17 -0.2 5,043,803 - BWI76_RS24195 0.17 +0.5 5,043,803 - BWI76_RS24195 0.17 -4.5 5,043,805 + BWI76_RS24195 0.18 +1.6 5,043,808 - BWI76_RS24195 0.18 -0.7 5,043,813 - BWI76_RS24195 0.18 +0.5 5,043,848 - BWI76_RS24195 0.21 -1.5 5,043,893 + BWI76_RS24195 0.24 -1.0 5,043,901 - BWI76_RS24195 0.24 +0.2 5,043,903 + BWI76_RS24195 0.25 -3.1 5,043,903 + BWI76_RS24195 0.25 +1.3 5,043,906 - BWI76_RS24195 0.25 -0.3 5,043,908 + BWI76_RS24195 0.25 +0.1 5,043,908 + -0.7 5,043,913 + BWI76_RS24195 0.25 -0.5 5,043,916 - BWI76_RS24195 0.26 -0.2 5,043,918 + BWI76_RS24195 0.26 +0.9 5,043,918 + BWI76_RS24195 0.26 -1.4 5,043,924 + BWI76_RS24195 0.26 -0.1 5,043,926 - BWI76_RS24195 0.26 +1.7 5,043,931 - BWI76_RS24195 0.27 -2.7 5,043,933 + BWI76_RS24195 0.27 +1.3 5,043,948 - BWI76_RS24195 0.28 -1.0 5,043,992 + BWI76_RS24195 0.31 -2.0 5,044,005 + BWI76_RS24195 0.32 -0.7 5,044,013 - +1.3 5,044,013 - +1.3 5,044,020 - +1.3 5,044,036 + BWI76_RS24195 0.34 -0.7 5,044,054 - BWI76_RS24195 0.35 +0.4 5,044,054 - BWI76_RS24195 0.35 +0.5 5,044,063 + BWI76_RS24195 0.36 -1.2 5,044,072 + BWI76_RS24195 0.37 -3.7 5,044,082 + BWI76_RS24195 0.38 +1.6 5,044,082 + BWI76_RS24195 0.38 -0.7 5,044,082 + BWI76_RS24195 0.38 -1.6 5,044,090 - BWI76_RS24195 0.38 +0.7 5,044,090 - BWI76_RS24195 0.38 +0.3 5,044,097 + BWI76_RS24195 0.39 -0.5 5,044,098 - BWI76_RS24195 0.39 +0.6 5,044,110 - BWI76_RS24195 0.40 -0.3 5,044,110 - BWI76_RS24195 0.40 -0.9 5,044,110 - BWI76_RS24195 0.40 -1.8 5,044,112 - BWI76_RS24195 0.40 -1.2 5,044,131 - BWI76_RS24195 0.41 -1.3 5,044,147 + BWI76_RS24195 0.42 +1.9 5,044,147 + +4.7 5,044,170 + BWI76_RS24195 0.44 +2.1 5,044,173 - BWI76_RS24195 0.44 -3.1 5,044,190 + BWI76_RS24195 0.45 +0.3 5,044,210 - BWI76_RS24195 0.47 +0.6 5,044,213 + BWI76_RS24195 0.47 -3.7 5,044,220 + BWI76_RS24195 0.47 +0.4 5,044,220 - BWI76_RS24195 0.47 -1.2 5,044,228 - BWI76_RS24195 0.48 +1.3 5,044,228 - BWI76_RS24195 0.48 +0.9
Or see this region's nucleotide sequence