Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23960 and BWI76_RS23965 are separated by 90 nucleotides BWI76_RS23965 and BWI76_RS23970 are separated by 150 nucleotides BWI76_RS23970 and BWI76_RS23975 are separated by 188 nucleotides
BWI76_RS23960: BWI76_RS23960 - oxidative stress defense protein, at 5,001,381 to 5,002,148
_RS23960
BWI76_RS23965: BWI76_RS23965 - arginine exporter protein, at 5,002,239 to 5,002,874
_RS23965
BWI76_RS23970: BWI76_RS23970 - mechanosensitive ion channel protein MscS, at 5,003,025 to 5,003,882
_RS23970
BWI76_RS23975: BWI76_RS23975 - class II fructose-bisphosphate aldolase, at 5,004,071 to 5,005,150
_RS23975
Position (kb) 5003
5004 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5002.184 kb on - strand at 5002.252 kb on + strand at 5002.355 kb on + strand, within BWI76_RS23965 at 5002.435 kb on - strand, within BWI76_RS23965 at 5003.009 kb on + strand at 5003.220 kb on - strand, within BWI76_RS23970 at 5003.415 kb on + strand, within BWI76_RS23970 at 5003.415 kb on + strand, within BWI76_RS23970 at 5003.538 kb on - strand, within BWI76_RS23970 at 5003.800 kb on + strand at 5003.813 kb on - strand at 5003.834 kb on + strand at 5003.936 kb on - strand at 5003.944 kb on + strand at 5003.944 kb on + strand at 5003.944 kb on + strand at 5003.944 kb on + strand at 5003.944 kb on + strand at 5003.944 kb on + strand at 5003.952 kb on - strand at 5003.952 kb on - strand at 5003.952 kb on - strand at 5003.955 kb on + strand at 5003.957 kb on + strand at 5003.963 kb on - strand at 5003.963 kb on - strand at 5003.987 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 5,002,184 - -1.2 5,002,252 + -0.7 5,002,355 + BWI76_RS23965 0.18 -0.9 5,002,435 - BWI76_RS23965 0.31 +0.7 5,003,009 + -0.3 5,003,220 - BWI76_RS23970 0.23 -0.3 5,003,415 + BWI76_RS23970 0.45 -2.2 5,003,415 + BWI76_RS23970 0.45 -1.3 5,003,538 - BWI76_RS23970 0.60 -1.3 5,003,800 + +0.7 5,003,813 - -0.3 5,003,834 + +0.7 5,003,936 - +0.2 5,003,944 + +2.6 5,003,944 + +0.4 5,003,944 + -0.3 5,003,944 + +0.8 5,003,944 + -2.7 5,003,944 + +0.1 5,003,952 - -2.0 5,003,952 - +0.2 5,003,952 - +1.4 5,003,955 + +0.1 5,003,957 + +1.1 5,003,963 - +1.3 5,003,963 - +0.0 5,003,987 + +0.6
Or see this region's nucleotide sequence