Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23670 and BWI76_RS23675 are separated by 109 nucleotides BWI76_RS23675 and BWI76_RS23680 are separated by 11 nucleotides BWI76_RS23680 and BWI76_RS23685 are separated by 36 nucleotides
BWI76_RS23670: BWI76_RS23670 - alkaline phosphatase, at 4,940,581 to 4,941,381
_RS23670
BWI76_RS23675: BWI76_RS23675 - allulose-6-phosphate 3-epimerase, at 4,941,491 to 4,942,180
_RS23675
BWI76_RS23680: BWI76_RS23680 - ribokinase, at 4,942,192 to 4,943,133
_RS23680
BWI76_RS23685: BWI76_RS23685 - MFS transporter, at 4,943,170 to 4,944,504
_RS23685
Position (kb) 4941
4942
4943 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4940.566 kb on + strand at 4940.566 kb on + strand at 4940.571 kb on + strand at 4940.576 kb on + strand at 4940.579 kb on - strand at 4940.623 kb on + strand at 4941.022 kb on - strand, within BWI76_RS23670 at 4941.207 kb on - strand, within BWI76_RS23670 at 4941.266 kb on + strand, within BWI76_RS23670 at 4941.297 kb on + strand, within BWI76_RS23670 at 4941.343 kb on + strand at 4941.644 kb on - strand, within BWI76_RS23675 at 4941.832 kb on + strand, within BWI76_RS23675 at 4941.851 kb on + strand, within BWI76_RS23675 at 4941.860 kb on + strand, within BWI76_RS23675 at 4941.870 kb on + strand, within BWI76_RS23675 at 4941.878 kb on - strand, within BWI76_RS23675 at 4941.973 kb on + strand, within BWI76_RS23675 at 4942.073 kb on - strand, within BWI76_RS23675 at 4942.074 kb on - strand, within BWI76_RS23675 at 4942.123 kb on - strand at 4942.162 kb on - strand at 4942.239 kb on + strand at 4942.428 kb on - strand, within BWI76_RS23680 at 4942.663 kb on + strand, within BWI76_RS23680 at 4942.717 kb on + strand, within BWI76_RS23680 at 4942.927 kb on - strand, within BWI76_RS23680 at 4942.927 kb on - strand, within BWI76_RS23680 at 4942.996 kb on + strand, within BWI76_RS23680 at 4943.142 kb on - strand at 4943.159 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 4,940,566 + -0.3 4,940,566 + +0.3 4,940,571 + +1.3 4,940,576 + -0.2 4,940,579 - -1.6 4,940,623 + -2.8 4,941,022 - BWI76_RS23670 0.55 +0.1 4,941,207 - BWI76_RS23670 0.78 -0.6 4,941,266 + BWI76_RS23670 0.86 -0.3 4,941,297 + BWI76_RS23670 0.89 +0.1 4,941,343 + -0.8 4,941,644 - BWI76_RS23675 0.22 -0.5 4,941,832 + BWI76_RS23675 0.49 +0.4 4,941,851 + BWI76_RS23675 0.52 -2.1 4,941,860 + BWI76_RS23675 0.53 -1.3 4,941,870 + BWI76_RS23675 0.55 -2.1 4,941,878 - BWI76_RS23675 0.56 -0.3 4,941,973 + BWI76_RS23675 0.70 -0.9 4,942,073 - BWI76_RS23675 0.84 +1.3 4,942,074 - BWI76_RS23675 0.84 +1.0 4,942,123 - +0.2 4,942,162 - +0.4 4,942,239 + +0.7 4,942,428 - BWI76_RS23680 0.25 -0.9 4,942,663 + BWI76_RS23680 0.50 -0.6 4,942,717 + BWI76_RS23680 0.56 +0.3 4,942,927 - BWI76_RS23680 0.78 -1.6 4,942,927 - BWI76_RS23680 0.78 +0.0 4,942,996 + BWI76_RS23680 0.85 -0.7 4,943,142 - -1.3 4,943,159 - -0.7
Or see this region's nucleotide sequence