Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22965 and BWI76_RS22970 overlap by 1 nucleotides BWI76_RS22970 and BWI76_RS22975 are separated by 139 nucleotides BWI76_RS22975 and BWI76_RS22980 are separated by 105 nucleotides
BWI76_RS22965: BWI76_RS22965 - cysteine sulfinate desulfinase, at 4,794,415 to 4,795,620
_RS22965
BWI76_RS22970: BWI76_RS22970 - cysteine desulfurase, sulfur acceptor subunit CsdE, at 4,795,620 to 4,796,060
_RS22970
BWI76_RS22975: BWI76_RS22975 - tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA, at 4,796,200 to 4,797,009
_RS22975
BWI76_RS22980: BWI76_RS22980 - murein transglycosylase A, at 4,797,115 to 4,798,212
_RS22980
Position (kb) 4796
4797
4798 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4795.425 kb on + strand, within BWI76_RS22965 at 4795.485 kb on - strand, within BWI76_RS22965 at 4795.627 kb on + strand at 4795.683 kb on - strand, within BWI76_RS22970 at 4796.063 kb on - strand at 4796.075 kb on + strand at 4796.151 kb on + strand at 4796.421 kb on - strand, within BWI76_RS22975 at 4796.438 kb on + strand, within BWI76_RS22975 at 4796.649 kb on - strand, within BWI76_RS22975 at 4796.985 kb on + strand at 4797.213 kb on - strand at 4797.261 kb on + strand, within BWI76_RS22980 at 4797.261 kb on + strand, within BWI76_RS22980 at 4797.269 kb on - strand, within BWI76_RS22980 at 4797.285 kb on + strand, within BWI76_RS22980 at 4797.370 kb on + strand, within BWI76_RS22980 at 4797.424 kb on - strand, within BWI76_RS22980 at 4797.987 kb on - strand, within BWI76_RS22980 at 4797.989 kb on + strand, within BWI76_RS22980
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 4,795,425 + BWI76_RS22965 0.84 -0.8 4,795,485 - BWI76_RS22965 0.89 +1.0 4,795,627 + +0.2 4,795,683 - BWI76_RS22970 0.14 -1.3 4,796,063 - +2.6 4,796,075 + +0.1 4,796,151 + -0.0 4,796,421 - BWI76_RS22975 0.27 -1.1 4,796,438 + BWI76_RS22975 0.29 -2.2 4,796,649 - BWI76_RS22975 0.55 -2.4 4,796,985 + -0.0 4,797,213 - -2.6 4,797,261 + BWI76_RS22980 0.13 +0.3 4,797,261 + BWI76_RS22980 0.13 +1.6 4,797,269 - BWI76_RS22980 0.14 -0.3 4,797,285 + BWI76_RS22980 0.15 -3.9 4,797,370 + BWI76_RS22980 0.23 +1.6 4,797,424 - BWI76_RS22980 0.28 -0.9 4,797,987 - BWI76_RS22980 0.79 -3.1 4,797,989 + BWI76_RS22980 0.80 -1.9
Or see this region's nucleotide sequence