Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20525 and BWI76_RS20530 are separated by 130 nucleotides BWI76_RS20530 and BWI76_RS20535 are separated by 48 nucleotides BWI76_RS20535 and BWI76_RS20540 are separated by 348 nucleotides
BWI76_RS20525: BWI76_RS20525 - glyceraldehyde 3-phosphate reductase, at 4,299,367 to 4,300,359
_RS20525
BWI76_RS20530: BWI76_RS20530 - hypothetical protein, at 4,300,490 to 4,300,849
_RS20530
BWI76_RS20535: BWI76_RS20535 - manganese transporter MntH, at 4,300,898 to 4,302,133
_RS20535
BWI76_RS20540: BWI76_RS20540 - NupC/NupG family nucleoside CNT transporter, at 4,302,482 to 4,303,684
_RS20540
Position (kb) 4300
4301
4302
4303 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4299.979 kb on - strand, within BWI76_RS20525 at 4300.019 kb on + strand, within BWI76_RS20525 at 4300.207 kb on + strand, within BWI76_RS20525 at 4300.215 kb on - strand, within BWI76_RS20525 at 4300.215 kb on - strand, within BWI76_RS20525 at 4300.241 kb on + strand, within BWI76_RS20525 at 4300.275 kb on + strand at 4300.417 kb on - strand at 4300.434 kb on + strand at 4300.447 kb on + strand at 4300.488 kb on - strand at 4300.563 kb on + strand, within BWI76_RS20530 at 4300.704 kb on - strand, within BWI76_RS20530 at 4300.726 kb on + strand, within BWI76_RS20530 at 4301.402 kb on + strand, within BWI76_RS20535 at 4302.305 kb on - strand at 4302.307 kb on + strand at 4302.479 kb on + strand at 4302.479 kb on + strand at 4302.646 kb on - strand, within BWI76_RS20540 at 4302.737 kb on + strand, within BWI76_RS20540 at 4302.854 kb on - strand, within BWI76_RS20540 at 4302.889 kb on + strand, within BWI76_RS20540
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 4,299,979 - BWI76_RS20525 0.62 -1.3 4,300,019 + BWI76_RS20525 0.66 -0.1 4,300,207 + BWI76_RS20525 0.85 +0.5 4,300,215 - BWI76_RS20525 0.85 +1.0 4,300,215 - BWI76_RS20525 0.85 +0.0 4,300,241 + BWI76_RS20525 0.88 +0.7 4,300,275 + +0.0 4,300,417 - -2.6 4,300,434 + +0.3 4,300,447 + +0.7 4,300,488 - -3.3 4,300,563 + BWI76_RS20530 0.20 +0.6 4,300,704 - BWI76_RS20530 0.59 -1.1 4,300,726 + BWI76_RS20530 0.66 -1.2 4,301,402 + BWI76_RS20535 0.41 -1.1 4,302,305 - -1.6 4,302,307 + +0.3 4,302,479 + -0.1 4,302,479 + -1.6 4,302,646 - BWI76_RS20540 0.14 -2.3 4,302,737 + BWI76_RS20540 0.21 -0.8 4,302,854 - BWI76_RS20540 0.31 -1.3 4,302,889 + BWI76_RS20540 0.34 -0.3
Or see this region's nucleotide sequence