Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20175

Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20170 and BWI76_RS20175 overlap by 4 nucleotidesBWI76_RS20175 and BWI76_RS20180 are separated by 2 nucleotides BWI76_RS20170: BWI76_RS20170 - NADH-quinone oxidoreductase subunit F, at 4,227,130 to 4,228,467 _RS20170 BWI76_RS20175: BWI76_RS20175 - NADH-quinone oxidoreductase subunit E, at 4,228,464 to 4,228,964 _RS20175 BWI76_RS20180: BWI76_RS20180 - NADH-quinone oxidoreductase subunit C/D, at 4,228,967 to 4,230,763 _RS20180 Position (kb) 4228 4229Strain fitness (log2 ratio) -2 -1 0 1 2at 4227.802 kb on - strand, within BWI76_RS20170at 4227.826 kb on + strand, within BWI76_RS20170at 4227.944 kb on + strand, within BWI76_RS20170at 4228.008 kb on - strand, within BWI76_RS20170at 4228.295 kb on - strand, within BWI76_RS20170at 4228.438 kb on + strandat 4228.521 kb on - strand, within BWI76_RS20175at 4229.509 kb on - strand, within BWI76_RS20180at 4229.533 kb on + strand, within BWI76_RS20180at 4229.573 kb on - strand, within BWI76_RS20180at 4229.753 kb on + strand, within BWI76_RS20180

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
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4,227,802 - BWI76_RS20170 0.50 -1.7
4,227,826 + BWI76_RS20170 0.52 -0.2
4,227,944 + BWI76_RS20170 0.61 -0.7
4,228,008 - BWI76_RS20170 0.66 -1.6
4,228,295 - BWI76_RS20170 0.87 -1.6
4,228,438 + -0.2
4,228,521 - BWI76_RS20175 0.11 +1.8
4,229,509 - BWI76_RS20180 0.30 -2.7
4,229,533 + BWI76_RS20180 0.31 +0.2
4,229,573 - BWI76_RS20180 0.34 -1.2
4,229,753 + BWI76_RS20180 0.44 +1.3

Or see this region's nucleotide sequence