Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20155 and BWI76_RS20160 are separated by 14 nucleotides BWI76_RS20160 and BWI76_RS20165 overlap by 4 nucleotides BWI76_RS20165 and BWI76_RS20170 are separated by 32 nucleotides
BWI76_RS20155: BWI76_RS20155 - NADH-quinone oxidoreductase subunit I, at 4,222,840 to 4,223,382
_RS20155
BWI76_RS20160: BWI76_RS20160 - NADH-quinone oxidoreductase subunit H, at 4,223,397 to 4,224,374
_RS20160
BWI76_RS20165: BWI76_RS20165 - NADH-quinone oxidoreductase subunit G, at 4,224,371 to 4,227,097
_RS20165
BWI76_RS20170: BWI76_RS20170 - NADH-quinone oxidoreductase subunit F, at 4,227,130 to 4,228,467
_RS20170
Position (kb) 4224
4225
4226
4227
4228 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4223.388 kb on + strand at 4223.447 kb on + strand at 4223.659 kb on + strand, within BWI76_RS20160 at 4223.804 kb on - strand, within BWI76_RS20160 at 4224.021 kb on + strand, within BWI76_RS20160 at 4224.218 kb on - strand, within BWI76_RS20160 at 4224.587 kb on - strand at 4225.133 kb on - strand, within BWI76_RS20165 at 4225.631 kb on + strand, within BWI76_RS20165 at 4226.002 kb on + strand, within BWI76_RS20165 at 4226.002 kb on + strand, within BWI76_RS20165 at 4226.418 kb on - strand, within BWI76_RS20165 at 4226.753 kb on - strand, within BWI76_RS20165 at 4226.753 kb on - strand, within BWI76_RS20165 at 4226.908 kb on - strand at 4227.802 kb on - strand, within BWI76_RS20170 at 4227.826 kb on + strand, within BWI76_RS20170 at 4227.944 kb on + strand, within BWI76_RS20170 at 4228.008 kb on - strand, within BWI76_RS20170
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 4,223,388 + +1.1 4,223,447 + -2.3 4,223,659 + BWI76_RS20160 0.27 -2.8 4,223,804 - BWI76_RS20160 0.42 -3.2 4,224,021 + BWI76_RS20160 0.64 -1.9 4,224,218 - BWI76_RS20160 0.84 -2.8 4,224,587 - +0.4 4,225,133 - BWI76_RS20165 0.28 -2.2 4,225,631 + BWI76_RS20165 0.46 -1.6 4,226,002 + BWI76_RS20165 0.60 -1.6 4,226,002 + BWI76_RS20165 0.60 +1.3 4,226,418 - BWI76_RS20165 0.75 -1.4 4,226,753 - BWI76_RS20165 0.87 +0.3 4,226,753 - BWI76_RS20165 0.87 +0.1 4,226,908 - -0.9 4,227,802 - BWI76_RS20170 0.50 -1.7 4,227,826 + BWI76_RS20170 0.52 -0.2 4,227,944 + BWI76_RS20170 0.61 -0.7 4,228,008 - BWI76_RS20170 0.66 -1.6
Or see this region's nucleotide sequence