Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19190 and BWI76_RS19195 overlap by 4 nucleotides BWI76_RS19195 and BWI76_RS19200 overlap by 4 nucleotides BWI76_RS19200 and BWI76_RS19205 overlap by 1 nucleotides
BWI76_RS19190: BWI76_RS19190 - oligopeptide/dipeptide ABC transporter ATPase, at 3,994,483 to 3,995,463
_RS19190
BWI76_RS19195: BWI76_RS19195 - nickel ABC transporter permease, at 3,995,460 to 3,996,306
_RS19195
BWI76_RS19200: BWI76_RS19200 - binding-protein-dependent transport system inner membrane protein, at 3,996,303 to 3,997,241
_RS19200
BWI76_RS19205: BWI76_RS19205 - putative proline iminopeptidase, at 3,997,241 to 3,998,140
_RS19205
Position (kb) 3996
3997
3998 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3995.398 kb on - strand at 3995.504 kb on + strand at 3995.621 kb on + strand, within BWI76_RS19195 at 3995.880 kb on - strand, within BWI76_RS19195 at 3995.929 kb on + strand, within BWI76_RS19195 at 3996.076 kb on - strand, within BWI76_RS19195 at 3996.078 kb on - strand, within BWI76_RS19195 at 3996.374 kb on + strand at 3996.433 kb on + strand, within BWI76_RS19200 at 3996.586 kb on + strand, within BWI76_RS19200 at 3996.654 kb on + strand, within BWI76_RS19200 at 3996.968 kb on + strand, within BWI76_RS19200 at 3997.091 kb on - strand, within BWI76_RS19200 at 3997.807 kb on + strand, within BWI76_RS19205 at 3997.906 kb on + strand, within BWI76_RS19205 at 3997.948 kb on + strand, within BWI76_RS19205 at 3998.075 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 3,995,398 - +0.0 3,995,504 + +0.6 3,995,621 + BWI76_RS19195 0.19 -1.8 3,995,880 - BWI76_RS19195 0.50 -1.0 3,995,929 + BWI76_RS19195 0.55 -0.4 3,996,076 - BWI76_RS19195 0.73 -0.5 3,996,078 - BWI76_RS19195 0.73 +0.1 3,996,374 + +0.4 3,996,433 + BWI76_RS19200 0.14 +1.2 3,996,586 + BWI76_RS19200 0.30 +1.6 3,996,654 + BWI76_RS19200 0.37 -1.2 3,996,968 + BWI76_RS19200 0.71 -3.6 3,997,091 - BWI76_RS19200 0.84 +0.5 3,997,807 + BWI76_RS19205 0.63 +1.5 3,997,906 + BWI76_RS19205 0.74 +0.6 3,997,948 + BWI76_RS19205 0.79 +0.6 3,998,075 + -1.3
Or see this region's nucleotide sequence