Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18025 and BWI76_RS18030 are separated by 22 nucleotides BWI76_RS18030 and BWI76_RS18035 are separated by 18 nucleotides BWI76_RS18035 and BWI76_RS18040 are separated by 2 nucleotides
BWI76_RS18025: BWI76_RS18025 - spore coat U domain-containing protein, at 3,746,154 to 3,746,705
_RS18025
BWI76_RS18030: BWI76_RS18030 - spore coat protein U, at 3,746,728 to 3,747,246
_RS18030
BWI76_RS18035: BWI76_RS18035 - fimbrial chaperone, at 3,747,265 to 3,748,029
_RS18035
BWI76_RS18040: BWI76_RS18040 - fimbrial assembly protein, at 3,748,032 to 3,750,362
_RS18040
Position (kb)
3747
3748
3749 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3746.268 kb on + strand, within BWI76_RS18025 at 3746.268 kb on + strand, within BWI76_RS18025 at 3746.356 kb on - strand, within BWI76_RS18025 at 3746.404 kb on + strand, within BWI76_RS18025 at 3746.475 kb on + strand, within BWI76_RS18025 at 3746.598 kb on + strand, within BWI76_RS18025 at 3746.691 kb on + strand at 3746.916 kb on + strand, within BWI76_RS18030 at 3746.928 kb on + strand, within BWI76_RS18030 at 3746.938 kb on + strand, within BWI76_RS18030 at 3746.994 kb on + strand, within BWI76_RS18030 at 3747.017 kb on + strand, within BWI76_RS18030 at 3747.048 kb on + strand, within BWI76_RS18030 at 3747.161 kb on - strand, within BWI76_RS18030 at 3747.163 kb on - strand, within BWI76_RS18030 at 3747.240 kb on - strand at 3747.383 kb on + strand, within BWI76_RS18035 at 3747.643 kb on + strand, within BWI76_RS18035 at 3747.677 kb on + strand, within BWI76_RS18035 at 3747.829 kb on + strand, within BWI76_RS18035 at 3747.834 kb on + strand, within BWI76_RS18035 at 3747.847 kb on - strand, within BWI76_RS18035 at 3748.082 kb on + strand at 3748.318 kb on - strand, within BWI76_RS18040 at 3748.633 kb on + strand, within BWI76_RS18040 at 3748.682 kb on - strand, within BWI76_RS18040 at 3748.689 kb on + strand, within BWI76_RS18040 at 3748.702 kb on - strand, within BWI76_RS18040 at 3748.858 kb on - strand, within BWI76_RS18040 at 3748.872 kb on - strand, within BWI76_RS18040 at 3748.874 kb on + strand, within BWI76_RS18040 at 3748.877 kb on - strand, within BWI76_RS18040 at 3748.978 kb on - strand, within BWI76_RS18040
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 3,746,268 + BWI76_RS18025 0.21 +0.2 3,746,268 + BWI76_RS18025 0.21 +1.3 3,746,356 - BWI76_RS18025 0.37 -1.8 3,746,404 + BWI76_RS18025 0.45 -1.6 3,746,475 + BWI76_RS18025 0.58 -1.8 3,746,598 + BWI76_RS18025 0.80 +0.7 3,746,691 + +0.1 3,746,916 + BWI76_RS18030 0.36 +0.4 3,746,928 + BWI76_RS18030 0.39 -0.3 3,746,938 + BWI76_RS18030 0.40 -2.1 3,746,994 + BWI76_RS18030 0.51 +0.3 3,747,017 + BWI76_RS18030 0.56 -1.4 3,747,048 + BWI76_RS18030 0.62 +0.0 3,747,161 - BWI76_RS18030 0.83 +0.7 3,747,163 - BWI76_RS18030 0.84 +0.8 3,747,240 - -1.6 3,747,383 + BWI76_RS18035 0.15 +0.1 3,747,643 + BWI76_RS18035 0.49 +0.4 3,747,677 + BWI76_RS18035 0.54 +1.7 3,747,829 + BWI76_RS18035 0.74 +1.5 3,747,834 + BWI76_RS18035 0.74 +0.5 3,747,847 - BWI76_RS18035 0.76 -2.7 3,748,082 + -1.1 3,748,318 - BWI76_RS18040 0.12 -0.3 3,748,633 + BWI76_RS18040 0.26 -2.6 3,748,682 - BWI76_RS18040 0.28 +0.6 3,748,689 + BWI76_RS18040 0.28 +0.5 3,748,702 - BWI76_RS18040 0.29 -0.4 3,748,858 - BWI76_RS18040 0.35 +0.3 3,748,872 - BWI76_RS18040 0.36 +1.3 3,748,874 + BWI76_RS18040 0.36 +0.5 3,748,877 - BWI76_RS18040 0.36 +1.8 3,748,978 - BWI76_RS18040 0.41 -0.8
Or see this region's nucleotide sequence