Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17930 and BWI76_RS17935 are separated by 41 nucleotides BWI76_RS17935 and BWI76_RS17940 overlap by 39 nucleotides BWI76_RS17940 and BWI76_RS17945 are separated by 461 nucleotides BWI76_RS17945 and BWI76_RS17950 are separated by 74 nucleotides BWI76_RS17950 and BWI76_RS17955 are separated by 159 nucleotides BWI76_RS17955 and BWI76_RS17960 are separated by 43 nucleotides
BWI76_RS17930: BWI76_RS17930 - cold-shock protein CspC, at 3,728,570 to 3,728,779
_RS17930
BWI76_RS17935: BWI76_RS17935 - DUF2627 domain-containing protein, at 3,728,821 to 3,728,937
_RS17935
BWI76_RS17940: BWI76_RS17940 - hypothetical protein, at 3,728,899 to 3,729,093
_RS17940
BWI76_RS17945: BWI76_RS17945 - hypothetical protein, at 3,729,555 to 3,729,845
_RS17945
BWI76_RS17950: BWI76_RS17950 - PhoP regulon feedback inhibition membrane protein MgrB, at 3,729,920 to 3,730,063
_RS17950
BWI76_RS17955: BWI76_RS17955 - hypothetical protein, at 3,730,223 to 3,730,462
_RS17955
BWI76_RS17960: BWI76_RS17960 - DNA-binding transcriptional regulator KdgR, at 3,730,506 to 3,731,297
_RS17960
Position (kb)
3729
3730 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3728.606 kb on - strand, within BWI76_RS17930 at 3728.684 kb on - strand, within BWI76_RS17930 at 3728.687 kb on - strand, within BWI76_RS17930 at 3728.733 kb on + strand, within BWI76_RS17930 at 3728.741 kb on - strand, within BWI76_RS17930 at 3728.763 kb on + strand at 3728.771 kb on - strand at 3728.771 kb on - strand at 3728.771 kb on - strand at 3728.797 kb on - strand at 3728.826 kb on - strand at 3728.860 kb on + strand, within BWI76_RS17935 at 3728.868 kb on - strand, within BWI76_RS17935 at 3728.868 kb on - strand, within BWI76_RS17935 at 3728.913 kb on - strand at 3728.943 kb on + strand, within BWI76_RS17940 at 3728.943 kb on - strand, within BWI76_RS17940 at 3728.946 kb on - strand, within BWI76_RS17940 at 3728.951 kb on - strand, within BWI76_RS17940 at 3728.951 kb on - strand, within BWI76_RS17940 at 3728.954 kb on - strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.041 kb on - strand, within BWI76_RS17940 at 3729.057 kb on + strand, within BWI76_RS17940 at 3729.156 kb on - strand at 3729.185 kb on - strand at 3729.202 kb on + strand at 3729.213 kb on + strand at 3729.235 kb on - strand at 3729.317 kb on + strand at 3729.317 kb on + strand at 3729.325 kb on - strand at 3729.352 kb on + strand at 3729.362 kb on + strand at 3729.362 kb on + strand at 3729.365 kb on + strand at 3729.370 kb on - strand at 3729.370 kb on - strand at 3729.388 kb on - strand at 3729.442 kb on + strand at 3729.442 kb on + strand at 3729.512 kb on + strand at 3729.527 kb on + strand at 3729.527 kb on + strand at 3729.527 kb on - strand at 3729.535 kb on - strand at 3729.542 kb on + strand at 3729.553 kb on + strand at 3729.553 kb on + strand at 3729.553 kb on + strand at 3729.561 kb on - strand at 3729.561 kb on - strand at 3729.561 kb on - strand at 3729.561 kb on - strand at 3729.561 kb on - strand at 3729.561 kb on - strand at 3729.561 kb on - strand at 3729.780 kb on - strand, within BWI76_RS17945 at 3729.805 kb on - strand, within BWI76_RS17945 at 3729.915 kb on - strand at 3730.096 kb on + strand at 3730.163 kb on + strand at 3730.316 kb on - strand, within BWI76_RS17955 at 3730.368 kb on + strand, within BWI76_RS17955 at 3730.419 kb on + strand, within BWI76_RS17955 at 3730.427 kb on - strand, within BWI76_RS17955 at 3730.667 kb on + strand, within BWI76_RS17960 at 3730.752 kb on - strand, within BWI76_RS17960
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 3,728,606 - BWI76_RS17930 0.17 +1.0 3,728,684 - BWI76_RS17930 0.54 +0.3 3,728,687 - BWI76_RS17930 0.56 -0.7 3,728,733 + BWI76_RS17930 0.78 -0.4 3,728,741 - BWI76_RS17930 0.81 +0.9 3,728,763 + -0.8 3,728,771 - -0.7 3,728,771 - -2.5 3,728,771 - +1.7 3,728,797 - +0.7 3,728,826 - -0.4 3,728,860 + BWI76_RS17935 0.33 +1.2 3,728,868 - BWI76_RS17935 0.40 +1.5 3,728,868 - BWI76_RS17935 0.40 -0.4 3,728,913 - -1.4 3,728,943 + BWI76_RS17940 0.23 +1.2 3,728,943 - BWI76_RS17940 0.23 +0.4 3,728,946 - BWI76_RS17940 0.24 -0.2 3,728,951 - BWI76_RS17940 0.27 -0.8 3,728,951 - BWI76_RS17940 0.27 +0.6 3,728,954 - BWI76_RS17940 0.28 -2.8 3,729,014 + BWI76_RS17940 0.59 +1.4 3,729,014 + BWI76_RS17940 0.59 +0.2 3,729,014 + BWI76_RS17940 0.59 -4.1 3,729,014 + BWI76_RS17940 0.59 -0.5 3,729,014 + +0.9 3,729,014 + BWI76_RS17940 0.59 -1.7 3,729,014 + BWI76_RS17940 0.59 -0.7 3,729,022 - BWI76_RS17940 0.63 -0.3 3,729,022 - BWI76_RS17940 0.63 +1.5 3,729,022 - BWI76_RS17940 0.63 -0.6 3,729,022 - BWI76_RS17940 0.63 -1.0 3,729,022 - BWI76_RS17940 0.63 +0.4 3,729,022 - BWI76_RS17940 0.63 -1.7 3,729,041 - BWI76_RS17940 0.73 -2.4 3,729,057 + BWI76_RS17940 0.81 -1.7 3,729,156 - -0.8 3,729,185 - +0.3 3,729,202 + -1.8 3,729,213 + -0.3 3,729,235 - +0.2 3,729,317 + +0.5 3,729,317 + -1.7 3,729,325 - +0.0 3,729,352 + -0.5 3,729,362 + -3.4 3,729,362 + +1.4 3,729,365 + -0.4 3,729,370 - +1.0 3,729,370 - +0.6 3,729,388 - -1.7 3,729,442 + -2.5 3,729,442 + +0.6 3,729,512 + +1.8 3,729,527 + -3.8 3,729,527 + -0.4 3,729,527 - +0.2 3,729,535 - +0.8 3,729,542 + +1.2 3,729,553 + -1.6 3,729,553 + -0.4 3,729,553 + -0.8 3,729,561 - -1.4 3,729,561 - +1.9 3,729,561 - -1.2 3,729,561 - +0.9 3,729,561 - -1.4 3,729,561 - +0.8 3,729,561 - +1.0 3,729,780 - BWI76_RS17945 0.77 -3.3 3,729,805 - BWI76_RS17945 0.86 -0.4 3,729,915 - +1.7 3,730,096 + -0.7 3,730,163 + +0.2 3,730,316 - BWI76_RS17955 0.39 -0.8 3,730,368 + BWI76_RS17955 0.60 +0.7 3,730,419 + BWI76_RS17955 0.82 +1.2 3,730,427 - BWI76_RS17955 0.85 +0.4 3,730,667 + BWI76_RS17960 0.20 -0.4 3,730,752 - BWI76_RS17960 0.31 +1.2
Or see this region's nucleotide sequence