Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17410 and BWI76_RS17415 are separated by 89 nucleotides BWI76_RS17415 and BWI76_RS17420 are separated by 39 nucleotides BWI76_RS17420 and BWI76_RS17425 are separated by 3 nucleotides BWI76_RS17425 and BWI76_RS17430 overlap by 4 nucleotides
BWI76_RS17410: BWI76_RS17410 - sodium-potassium/proton antiporter ChaA, at 3,611,392 to 3,612,492
_RS17410
BWI76_RS17415: BWI76_RS17415 - 3-deoxy-8-phosphooctulonate synthase, at 3,612,582 to 3,613,436
_RS17415
BWI76_RS17420: BWI76_RS17420 - hypothetical protein, at 3,613,476 to 3,614,288
_RS17420
BWI76_RS17425: BWI76_RS17425 - hypothetical protein, at 3,614,292 to 3,614,684
_RS17425
BWI76_RS17430: BWI76_RS17430 - protein-(glutamine-N5) methyltransferase, release factor-specific, at 3,614,681 to 3,615,520
_RS17430
Position (kb) 3613
3614
3615 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 3613.611 kb on - strand, within BWI76_RS17420 at 3613.979 kb on - strand, within BWI76_RS17420 at 3614.032 kb on - strand, within BWI76_RS17420 at 3614.462 kb on + strand, within BWI76_RS17425 at 3614.470 kb on - strand, within BWI76_RS17425 at 3614.653 kb on + strand at 3614.805 kb on - strand, within BWI76_RS17430
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 3,613,611 - BWI76_RS17420 0.17 -0.6 3,613,979 - BWI76_RS17420 0.62 -0.4 3,614,032 - BWI76_RS17420 0.68 -0.3 3,614,462 + BWI76_RS17425 0.43 -3.5 3,614,470 - BWI76_RS17425 0.45 +3.3 3,614,653 + +1.5 3,614,805 - BWI76_RS17430 0.15 +0.1
Or see this region's nucleotide sequence