Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17085 and BWI76_RS17090 are separated by 126 nucleotides BWI76_RS17090 and BWI76_RS17095 are separated by 81 nucleotides BWI76_RS17095 and BWI76_RS17100 overlap by 1 nucleotides BWI76_RS17100 and BWI76_RS17105 are separated by 224 nucleotides
BWI76_RS17085: BWI76_RS17085 - hypothetical protein, at 3,545,195 to 3,546,637
_RS17085
BWI76_RS17090: BWI76_RS17090 - NLP/P60 protein, at 3,546,764 to 3,547,228
_RS17090
BWI76_RS17095: BWI76_RS17095 - vitamin B12 ABC transporter ATP-binding protein BtuD, at 3,547,310 to 3,548,059
_RS17095
BWI76_RS17100: BWI76_RS17100 - glutathione peroxidase, at 3,548,059 to 3,548,610
_RS17100
BWI76_RS17105: BWI76_RS17105 - methionine ABC transporter substrate-binding protein MetQ, at 3,548,835 to 3,549,635
_RS17105
Position (kb)
3547
3548
3549 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3546.374 kb on - strand, within BWI76_RS17085 at 3546.465 kb on + strand, within BWI76_RS17085 at 3546.547 kb on - strand at 3547.496 kb on + strand, within BWI76_RS17095 at 3547.564 kb on + strand, within BWI76_RS17095 at 3547.695 kb on + strand, within BWI76_RS17095 at 3547.766 kb on - strand, within BWI76_RS17095 at 3547.766 kb on - strand, within BWI76_RS17095 at 3547.850 kb on + strand, within BWI76_RS17095 at 3548.026 kb on + strand at 3548.387 kb on - strand, within BWI76_RS17100 at 3548.402 kb on - strand, within BWI76_RS17100 at 3548.654 kb on + strand at 3548.743 kb on - strand at 3548.856 kb on + strand at 3548.868 kb on + strand at 3548.882 kb on + strand at 3548.890 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 3,546,374 - BWI76_RS17085 0.82 -1.1 3,546,465 + BWI76_RS17085 0.88 -0.9 3,546,547 - +1.1 3,547,496 + BWI76_RS17095 0.25 +0.3 3,547,564 + BWI76_RS17095 0.34 -4.7 3,547,695 + BWI76_RS17095 0.51 +0.6 3,547,766 - BWI76_RS17095 0.61 -1.5 3,547,766 - BWI76_RS17095 0.61 -0.6 3,547,850 + BWI76_RS17095 0.72 -0.0 3,548,026 + -0.9 3,548,387 - BWI76_RS17100 0.59 +0.9 3,548,402 - BWI76_RS17100 0.62 +0.0 3,548,654 + -1.5 3,548,743 - +1.1 3,548,856 + -0.7 3,548,868 + -0.4 3,548,882 + -2.9 3,548,890 - -2.3
Or see this region's nucleotide sequence