Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17090

Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17085 and BWI76_RS17090 are separated by 126 nucleotidesBWI76_RS17090 and BWI76_RS17095 are separated by 81 nucleotidesBWI76_RS17095 and BWI76_RS17100 overlap by 1 nucleotides BWI76_RS17085: BWI76_RS17085 - hypothetical protein, at 3,545,195 to 3,546,637 _RS17085 BWI76_RS17090: BWI76_RS17090 - NLP/P60 protein, at 3,546,764 to 3,547,228 _RS17090 BWI76_RS17095: BWI76_RS17095 - vitamin B12 ABC transporter ATP-binding protein BtuD, at 3,547,310 to 3,548,059 _RS17095 BWI76_RS17100: BWI76_RS17100 - glutathione peroxidase, at 3,548,059 to 3,548,610 _RS17100 Position (kb) 3546 3547 3548Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3546.020 kb on - strand, within BWI76_RS17085at 3546.160 kb on + strand, within BWI76_RS17085at 3546.257 kb on + strand, within BWI76_RS17085at 3546.374 kb on - strand, within BWI76_RS17085at 3546.465 kb on + strand, within BWI76_RS17085at 3546.547 kb on - strandat 3547.496 kb on + strand, within BWI76_RS17095at 3547.564 kb on + strand, within BWI76_RS17095at 3547.695 kb on + strand, within BWI76_RS17095at 3547.766 kb on - strand, within BWI76_RS17095at 3547.766 kb on - strand, within BWI76_RS17095at 3547.850 kb on + strand, within BWI76_RS17095at 3548.026 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
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3,546,020 - BWI76_RS17085 0.57 -1.1
3,546,160 + BWI76_RS17085 0.67 -0.5
3,546,257 + BWI76_RS17085 0.74 -2.0
3,546,374 - BWI76_RS17085 0.82 -1.1
3,546,465 + BWI76_RS17085 0.88 -0.9
3,546,547 - +1.1
3,547,496 + BWI76_RS17095 0.25 +0.3
3,547,564 + BWI76_RS17095 0.34 -4.7
3,547,695 + BWI76_RS17095 0.51 +0.6
3,547,766 - BWI76_RS17095 0.61 -1.5
3,547,766 - BWI76_RS17095 0.61 -0.6
3,547,850 + BWI76_RS17095 0.72 -0.0
3,548,026 + -0.9

Or see this region's nucleotide sequence