Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15030 and BWI76_RS15035 overlap by 16 nucleotides BWI76_RS15035 and BWI76_RS15040 are separated by 31 nucleotides BWI76_RS15040 and BWI76_RS15045 are separated by 92 nucleotides BWI76_RS15045 and BWI76_RS15050 overlap by 18 nucleotides
BWI76_RS15030: BWI76_RS15030 - ABC transporter, at 3,146,456 to 3,147,247
_RS15030
BWI76_RS15035: BWI76_RS15035 - metal ABC transporter permease, at 3,147,232 to 3,147,903
_RS15035
BWI76_RS15040: BWI76_RS15040 - putative 2-nitropropane dioxygenase, at 3,147,935 to 3,148,981
_RS15040
BWI76_RS15045: BWI76_RS15045 - LysR family transcriptional regulator, at 3,149,074 to 3,149,910
_RS15045
BWI76_RS15050: BWI76_RS15050 - sugar transporter, at 3,149,893 to 3,151,080
_RS15050
Position (kb) 3147
3148
3149 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3147.155 kb on - strand, within BWI76_RS15030 at 3147.407 kb on - strand, within BWI76_RS15035 at 3147.752 kb on + strand, within BWI76_RS15035 at 3148.203 kb on + strand, within BWI76_RS15040 at 3148.263 kb on + strand, within BWI76_RS15040 at 3148.302 kb on - strand, within BWI76_RS15040 at 3148.304 kb on + strand, within BWI76_RS15040 at 3148.304 kb on + strand, within BWI76_RS15040 at 3148.317 kb on + strand, within BWI76_RS15040 at 3148.389 kb on - strand, within BWI76_RS15040 at 3148.478 kb on - strand, within BWI76_RS15040 at 3148.509 kb on - strand, within BWI76_RS15040 at 3148.603 kb on + strand, within BWI76_RS15040 at 3149.322 kb on - strand, within BWI76_RS15045 at 3149.332 kb on - strand, within BWI76_RS15045 at 3149.625 kb on + strand, within BWI76_RS15045 at 3149.848 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 3,147,155 - BWI76_RS15030 0.88 -0.2 3,147,407 - BWI76_RS15035 0.26 -0.1 3,147,752 + BWI76_RS15035 0.77 -2.2 3,148,203 + BWI76_RS15040 0.26 +1.1 3,148,263 + BWI76_RS15040 0.31 +0.9 3,148,302 - BWI76_RS15040 0.35 +0.3 3,148,304 + BWI76_RS15040 0.35 +1.3 3,148,304 + BWI76_RS15040 0.35 -0.3 3,148,317 + BWI76_RS15040 0.36 +0.1 3,148,389 - BWI76_RS15040 0.43 +0.2 3,148,478 - BWI76_RS15040 0.52 -1.0 3,148,509 - BWI76_RS15040 0.55 +0.6 3,148,603 + BWI76_RS15040 0.64 +0.3 3,149,322 - BWI76_RS15045 0.30 +0.8 3,149,332 - BWI76_RS15045 0.31 +0.0 3,149,625 + BWI76_RS15045 0.66 -2.8 3,149,848 - -2.6
Or see this region's nucleotide sequence