Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14800 and BWI76_RS14805 are separated by 302 nucleotides BWI76_RS14805 and BWI76_RS14810 are separated by 4 nucleotides BWI76_RS14810 and BWI76_RS14815 are separated by 21 nucleotides BWI76_RS14815 and BWI76_RS14820 are separated by 98 nucleotides
BWI76_RS14800: BWI76_RS14800 - non-heme chloroperoxidase, at 3,102,519 to 3,103,355
_RS14800
BWI76_RS14805: BWI76_RS14805 - hypothetical protein, at 3,103,658 to 3,104,092
_RS14805
BWI76_RS14810: BWI76_RS14810 - carboxymuconolactone decarboxylase, at 3,104,097 to 3,104,897
_RS14810
BWI76_RS14815: BWI76_RS14815 - MdaB protein, at 3,104,919 to 3,105,515
_RS14815
BWI76_RS14820: BWI76_RS14820 - LysR family transcriptional regulator, at 3,105,614 to 3,106,522
_RS14820
Position (kb) 3104
3105 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3103.173 kb on + strand, within BWI76_RS14800 at 3103.295 kb on + strand at 3103.430 kb on + strand at 3103.595 kb on - strand at 3103.841 kb on + strand, within BWI76_RS14805 at 3103.955 kb on + strand, within BWI76_RS14805 at 3104.076 kb on - strand at 3104.359 kb on + strand, within BWI76_RS14810 at 3104.410 kb on - strand, within BWI76_RS14810 at 3104.544 kb on - strand, within BWI76_RS14810 at 3104.648 kb on + strand, within BWI76_RS14810 at 3104.656 kb on - strand, within BWI76_RS14810 at 3104.702 kb on - strand, within BWI76_RS14810 at 3104.726 kb on - strand, within BWI76_RS14810 at 3104.736 kb on - strand, within BWI76_RS14810 at 3104.790 kb on + strand, within BWI76_RS14810 at 3104.790 kb on + strand, within BWI76_RS14810 at 3105.041 kb on + strand, within BWI76_RS14815 at 3105.059 kb on + strand, within BWI76_RS14815 at 3105.067 kb on - strand, within BWI76_RS14815 at 3105.067 kb on - strand, within BWI76_RS14815 at 3105.393 kb on - strand, within BWI76_RS14815 at 3105.432 kb on + strand, within BWI76_RS14815 at 3105.475 kb on + strand at 3105.676 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 3,103,173 + BWI76_RS14800 0.78 +0.5 3,103,295 + +0.0 3,103,430 + +0.9 3,103,595 - +1.0 3,103,841 + BWI76_RS14805 0.42 +1.2 3,103,955 + BWI76_RS14805 0.68 +2.0 3,104,076 - -0.0 3,104,359 + BWI76_RS14810 0.33 -2.9 3,104,410 - BWI76_RS14810 0.39 +0.6 3,104,544 - BWI76_RS14810 0.56 -2.8 3,104,648 + BWI76_RS14810 0.69 +0.8 3,104,656 - BWI76_RS14810 0.70 -0.9 3,104,702 - BWI76_RS14810 0.76 -1.1 3,104,726 - BWI76_RS14810 0.79 +0.2 3,104,736 - BWI76_RS14810 0.80 -1.1 3,104,790 + BWI76_RS14810 0.87 -0.8 3,104,790 + BWI76_RS14810 0.87 -2.0 3,105,041 + BWI76_RS14815 0.20 -1.1 3,105,059 + BWI76_RS14815 0.23 +0.6 3,105,067 - BWI76_RS14815 0.25 -0.2 3,105,067 - BWI76_RS14815 0.25 -0.1 3,105,393 - BWI76_RS14815 0.79 +1.2 3,105,432 + BWI76_RS14815 0.86 -1.0 3,105,475 + +0.4 3,105,676 + +0.0
Or see this region's nucleotide sequence