Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13820 and BWI76_RS13825 overlap by 8 nucleotides BWI76_RS13825 and BWI76_RS13830 are separated by 152 nucleotides BWI76_RS13830 and BWI76_RS13835 are separated by 184 nucleotides BWI76_RS13835 and BWI76_RS13840 are separated by 236 nucleotides
BWI76_RS13820: BWI76_RS13820 - hypothetical protein, at 2,883,589 to 2,884,269
_RS13820
BWI76_RS13825: BWI76_RS13825 - hypothetical protein, at 2,884,262 to 2,884,957
_RS13825
BWI76_RS13830: BWI76_RS13830 - NAD-dependent malic enzyme, at 2,885,110 to 2,886,807
_RS13830
BWI76_RS13835: BWI76_RS13835 - 30S ribosomal protein S22, at 2,886,992 to 2,887,132
_RS13835
BWI76_RS13840: BWI76_RS13840 - MBL fold metallo-hydrolase, at 2,887,369 to 2,888,220
_RS13840
Position (kb)
2885
2886
2887 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2884.352 kb on - strand, within BWI76_RS13825 at 2884.985 kb on + strand at 2885.034 kb on + strand at 2885.034 kb on + strand at 2885.034 kb on + strand at 2885.068 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.078 kb on - strand at 2885.078 kb on - strand at 2885.078 kb on - strand at 2885.078 kb on - strand at 2885.142 kb on - strand at 2885.155 kb on - strand at 2885.176 kb on - strand at 2885.222 kb on + strand at 2885.222 kb on + strand at 2885.224 kb on + strand at 2885.230 kb on - strand at 2885.230 kb on - strand at 2885.230 kb on - strand at 2885.286 kb on - strand, within BWI76_RS13830 at 2885.410 kb on + strand, within BWI76_RS13830 at 2885.428 kb on - strand, within BWI76_RS13830 at 2885.587 kb on + strand, within BWI76_RS13830 at 2885.774 kb on - strand, within BWI76_RS13830 at 2886.269 kb on + strand at 2886.500 kb on - strand, within BWI76_RS13830 at 2886.526 kb on + strand, within BWI76_RS13830 at 2886.539 kb on + strand, within BWI76_RS13830 at 2886.702 kb on + strand at 2886.715 kb on + strand at 2886.750 kb on + strand at 2886.760 kb on + strand at 2886.761 kb on + strand at 2886.877 kb on - strand at 2886.886 kb on + strand at 2886.886 kb on + strand at 2886.886 kb on + strand at 2886.894 kb on - strand at 2886.894 kb on - strand at 2886.909 kb on - strand at 2887.140 kb on + strand at 2887.226 kb on + strand at 2887.327 kb on - strand at 2887.425 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 2,884,352 - BWI76_RS13825 0.13 +0.2 2,884,985 + -2.1 2,885,034 + -0.6 2,885,034 + +1.2 2,885,034 + +1.7 2,885,068 + +1.7 2,885,070 + +0.9 2,885,070 + -1.2 2,885,070 + -3.0 2,885,070 + -3.9 2,885,070 + +0.9 2,885,070 + +0.2 2,885,070 + -1.0 2,885,070 + +1.3 2,885,070 + +0.8 2,885,070 + -0.6 2,885,070 + -1.6 2,885,070 + +0.4 2,885,070 + -0.7 2,885,070 + +0.4 2,885,070 + -2.6 2,885,070 + +1.1 2,885,070 + -2.2 2,885,070 + -0.4 2,885,070 + -1.6 2,885,070 + +0.0 2,885,070 + -0.3 2,885,070 + +2.0 2,885,070 + +0.4 2,885,070 + +0.6 2,885,070 + -1.8 2,885,070 + +1.3 2,885,070 + +1.1 2,885,070 + -1.0 2,885,070 + +0.6 2,885,070 + +0.1 2,885,070 + +1.0 2,885,070 + -3.9 2,885,070 + -2.2 2,885,070 + -3.5 2,885,070 + -0.7 2,885,078 - +0.6 2,885,078 - -2.0 2,885,078 - +1.1 2,885,078 - +1.2 2,885,142 - +0.8 2,885,155 - -0.1 2,885,176 - +0.9 2,885,222 + +1.0 2,885,222 + -0.0 2,885,224 + +2.4 2,885,230 - +1.5 2,885,230 - -0.5 2,885,230 - +1.0 2,885,286 - BWI76_RS13830 0.10 +0.3 2,885,410 + BWI76_RS13830 0.18 -0.4 2,885,428 - BWI76_RS13830 0.19 +3.5 2,885,587 + BWI76_RS13830 0.28 -1.2 2,885,774 - BWI76_RS13830 0.39 +0.2 2,886,269 + -0.4 2,886,500 - BWI76_RS13830 0.82 -0.8 2,886,526 + BWI76_RS13830 0.83 -3.7 2,886,539 + BWI76_RS13830 0.84 -3.6 2,886,702 + +0.3 2,886,715 + +0.3 2,886,750 + -1.2 2,886,760 + +0.9 2,886,761 + -1.5 2,886,877 - -1.7 2,886,886 + +0.7 2,886,886 + +0.7 2,886,886 + -3.4 2,886,894 - -1.1 2,886,894 - +0.7 2,886,909 - -0.3 2,887,140 + +1.1 2,887,226 + +1.2 2,887,327 - -0.4 2,887,425 - +0.6
Or see this region's nucleotide sequence