Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11530 and BWI76_RS11535 are separated by 324 nucleotides BWI76_RS11535 and BWI76_RS11540 are separated by 26 nucleotides
BWI76_RS11530: BWI76_RS11530 - chitinase, at 2,425,054 to 2,426,307
_RS11530
BWI76_RS11535: BWI76_RS11535 - putative oxidoreductase, Zn-dependent and NAD(P)-binding protein, at 2,426,632 to 2,427,708
_RS11535
BWI76_RS11540: BWI76_RS11540 - MFS transporter, at 2,427,735 to 2,429,114
_RS11540
Position (kb)
2426
2427
2428 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2425.646 kb on - strand, within BWI76_RS11530 at 2425.753 kb on + strand, within BWI76_RS11530 at 2425.924 kb on + strand, within BWI76_RS11530 at 2426.128 kb on - strand, within BWI76_RS11530 at 2426.467 kb on + strand at 2427.190 kb on - strand, within BWI76_RS11535 at 2427.342 kb on + strand, within BWI76_RS11535 at 2427.510 kb on + strand, within BWI76_RS11535 at 2427.579 kb on - strand, within BWI76_RS11535 at 2427.617 kb on + strand at 2427.951 kb on - strand, within BWI76_RS11540 at 2428.061 kb on + strand, within BWI76_RS11540 at 2428.177 kb on + strand, within BWI76_RS11540 at 2428.207 kb on + strand, within BWI76_RS11540 at 2428.228 kb on - strand, within BWI76_RS11540 at 2428.240 kb on + strand, within BWI76_RS11540 at 2428.240 kb on + strand, within BWI76_RS11540 at 2428.240 kb on + strand, within BWI76_RS11540 at 2428.248 kb on - strand, within BWI76_RS11540 at 2428.248 kb on - strand, within BWI76_RS11540 at 2428.276 kb on + strand, within BWI76_RS11540 at 2428.276 kb on - strand, within BWI76_RS11540 at 2428.308 kb on + strand at 2428.374 kb on - strand, within BWI76_RS11540 at 2428.407 kb on + strand, within BWI76_RS11540 at 2428.413 kb on - strand, within BWI76_RS11540 at 2428.471 kb on + strand, within BWI76_RS11540 at 2428.490 kb on + strand, within BWI76_RS11540 at 2428.490 kb on + strand, within BWI76_RS11540 at 2428.503 kb on - strand, within BWI76_RS11540 at 2428.505 kb on + strand, within BWI76_RS11540 at 2428.513 kb on + strand, within BWI76_RS11540 at 2428.513 kb on + strand, within BWI76_RS11540 at 2428.513 kb on - strand, within BWI76_RS11540 at 2428.521 kb on - strand, within BWI76_RS11540 at 2428.543 kb on + strand, within BWI76_RS11540 at 2428.551 kb on - strand, within BWI76_RS11540 at 2428.551 kb on - strand at 2428.551 kb on - strand, within BWI76_RS11540 at 2428.557 kb on + strand, within BWI76_RS11540 at 2428.557 kb on + strand, within BWI76_RS11540 at 2428.569 kb on + strand, within BWI76_RS11540 at 2428.569 kb on + strand, within BWI76_RS11540 at 2428.569 kb on + strand, within BWI76_RS11540 at 2428.572 kb on + strand, within BWI76_RS11540 at 2428.577 kb on - strand, within BWI76_RS11540
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 2,425,646 - BWI76_RS11530 0.47 -3.4 2,425,753 + BWI76_RS11530 0.56 -2.0 2,425,924 + BWI76_RS11530 0.69 +0.2 2,426,128 - BWI76_RS11530 0.86 -1.1 2,426,467 + +0.7 2,427,190 - BWI76_RS11535 0.52 -1.3 2,427,342 + BWI76_RS11535 0.66 -1.3 2,427,510 + BWI76_RS11535 0.82 -0.4 2,427,579 - BWI76_RS11535 0.88 -1.4 2,427,617 + +0.7 2,427,951 - BWI76_RS11540 0.16 -0.2 2,428,061 + BWI76_RS11540 0.24 +0.0 2,428,177 + BWI76_RS11540 0.32 +0.5 2,428,207 + BWI76_RS11540 0.34 -2.8 2,428,228 - BWI76_RS11540 0.36 +2.1 2,428,240 + BWI76_RS11540 0.37 +0.0 2,428,240 + BWI76_RS11540 0.37 -0.2 2,428,240 + BWI76_RS11540 0.37 +1.3 2,428,248 - BWI76_RS11540 0.37 +1.0 2,428,248 - BWI76_RS11540 0.37 -0.2 2,428,276 + BWI76_RS11540 0.39 -0.1 2,428,276 - BWI76_RS11540 0.39 -0.1 2,428,308 + -0.7 2,428,374 - BWI76_RS11540 0.46 -2.8 2,428,407 + BWI76_RS11540 0.49 +0.1 2,428,413 - BWI76_RS11540 0.49 -0.7 2,428,471 + BWI76_RS11540 0.53 -3.7 2,428,490 + BWI76_RS11540 0.55 -0.3 2,428,490 + BWI76_RS11540 0.55 -2.1 2,428,503 - BWI76_RS11540 0.56 -3.1 2,428,505 + BWI76_RS11540 0.56 +0.7 2,428,513 + BWI76_RS11540 0.56 -2.6 2,428,513 + BWI76_RS11540 0.56 -1.8 2,428,513 - BWI76_RS11540 0.56 -1.3 2,428,521 - BWI76_RS11540 0.57 -1.1 2,428,543 + BWI76_RS11540 0.59 -3.5 2,428,551 - BWI76_RS11540 0.59 -1.1 2,428,551 - -0.3 2,428,551 - BWI76_RS11540 0.59 +3.3 2,428,557 + BWI76_RS11540 0.60 -1.1 2,428,557 + BWI76_RS11540 0.60 +0.1 2,428,569 + BWI76_RS11540 0.60 +1.3 2,428,569 + BWI76_RS11540 0.60 -1.3 2,428,569 + BWI76_RS11540 0.60 -0.3 2,428,572 + BWI76_RS11540 0.61 -0.7 2,428,577 - BWI76_RS11540 0.61 -1.3
Or see this region's nucleotide sequence