Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08475 and BWI76_RS08480 overlap by 1 nucleotides BWI76_RS08480 and BWI76_RS08485 are separated by 16 nucleotides
BWI76_RS08475: BWI76_RS08475 - methylaspartate ammonia-lyase, at 1,794,339 to 1,795,580
_RS08475
BWI76_RS08480: BWI76_RS08480 - methylaspartate mutase subunit E, at 1,795,580 to 1,797,025
_RS08480
BWI76_RS08485: BWI76_RS08485 - glutamate mutase, at 1,797,042 to 1,798,430
_RS08485
Position (kb)
1795
1796
1797
1798 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1794.594 kb on - strand at 1794.646 kb on - strand, within BWI76_RS08475 at 1794.654 kb on - strand at 1794.654 kb on - strand, within BWI76_RS08475 at 1794.729 kb on - strand, within BWI76_RS08475 at 1794.763 kb on + strand, within BWI76_RS08475 at 1794.773 kb on + strand, within BWI76_RS08475 at 1794.773 kb on + strand, within BWI76_RS08475 at 1794.993 kb on - strand, within BWI76_RS08475 at 1795.257 kb on + strand, within BWI76_RS08475 at 1795.258 kb on - strand, within BWI76_RS08475 at 1795.370 kb on - strand, within BWI76_RS08475 at 1795.454 kb on + strand, within BWI76_RS08475 at 1795.622 kb on - strand at 1795.704 kb on - strand at 1795.720 kb on + strand at 1795.835 kb on + strand, within BWI76_RS08480 at 1795.851 kb on - strand, within BWI76_RS08480 at 1795.886 kb on + strand, within BWI76_RS08480 at 1795.894 kb on - strand, within BWI76_RS08480 at 1795.894 kb on - strand, within BWI76_RS08480 at 1795.979 kb on - strand, within BWI76_RS08480 at 1796.394 kb on - strand, within BWI76_RS08480 at 1796.789 kb on - strand, within BWI76_RS08480 at 1796.789 kb on - strand, within BWI76_RS08480 at 1796.847 kb on - strand, within BWI76_RS08480 at 1796.859 kb on + strand, within BWI76_RS08480 at 1796.867 kb on - strand, within BWI76_RS08480 at 1796.867 kb on - strand, within BWI76_RS08480 at 1796.867 kb on - strand, within BWI76_RS08480 at 1796.975 kb on - strand at 1797.024 kb on + strand at 1797.057 kb on + strand at 1797.080 kb on + strand at 1797.085 kb on + strand at 1797.085 kb on + strand at 1797.207 kb on + strand, within BWI76_RS08485 at 1797.315 kb on + strand, within BWI76_RS08485 at 1797.912 kb on + strand, within BWI76_RS08485 at 1798.009 kb on - strand, within BWI76_RS08485 at 1798.011 kb on + strand, within BWI76_RS08485
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 1,794,594 - -0.4 1,794,646 - BWI76_RS08475 0.25 -1.8 1,794,654 - +1.2 1,794,654 - BWI76_RS08475 0.25 +0.5 1,794,729 - BWI76_RS08475 0.31 -2.0 1,794,763 + BWI76_RS08475 0.34 +0.8 1,794,773 + BWI76_RS08475 0.35 +1.1 1,794,773 + BWI76_RS08475 0.35 -0.5 1,794,993 - BWI76_RS08475 0.53 -1.0 1,795,257 + BWI76_RS08475 0.74 -0.8 1,795,258 - BWI76_RS08475 0.74 -1.6 1,795,370 - BWI76_RS08475 0.83 -2.0 1,795,454 + BWI76_RS08475 0.90 +0.7 1,795,622 - +1.5 1,795,704 - +0.1 1,795,720 + -0.9 1,795,835 + BWI76_RS08480 0.18 -2.6 1,795,851 - BWI76_RS08480 0.19 -3.3 1,795,886 + BWI76_RS08480 0.21 -2.2 1,795,894 - BWI76_RS08480 0.22 +1.1 1,795,894 - BWI76_RS08480 0.22 -0.2 1,795,979 - BWI76_RS08480 0.28 +0.6 1,796,394 - BWI76_RS08480 0.56 +0.6 1,796,789 - BWI76_RS08480 0.84 +0.2 1,796,789 - BWI76_RS08480 0.84 +0.2 1,796,847 - BWI76_RS08480 0.88 -0.4 1,796,859 + BWI76_RS08480 0.88 -0.0 1,796,867 - BWI76_RS08480 0.89 -0.4 1,796,867 - BWI76_RS08480 0.89 -0.2 1,796,867 - BWI76_RS08480 0.89 -0.6 1,796,975 - +0.2 1,797,024 + -2.9 1,797,057 + -0.1 1,797,080 + +0.2 1,797,085 + -1.0 1,797,085 + +0.7 1,797,207 + BWI76_RS08485 0.12 +1.3 1,797,315 + BWI76_RS08485 0.20 +0.2 1,797,912 + BWI76_RS08485 0.63 -0.2 1,798,009 - BWI76_RS08485 0.70 -2.3 1,798,011 + BWI76_RS08485 0.70 -3.3
Or see this region's nucleotide sequence