Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08360 and BWI76_RS08365 are separated by 62 nucleotides BWI76_RS08365 and BWI76_RS08370 overlap by 4 nucleotides BWI76_RS08370 and BWI76_RS08375 are separated by 9 nucleotides BWI76_RS08375 and BWI76_RS08380 are separated by 15 nucleotides
BWI76_RS08360: BWI76_RS08360 - LamB/YcsF family protein, at 1,769,645 to 1,770,391
_RS08360
BWI76_RS08365: BWI76_RS08365 - membrane protein, at 1,770,454 to 1,771,179
_RS08365
BWI76_RS08370: BWI76_RS08370 - membrane protein, at 1,771,176 to 1,772,171
_RS08370
BWI76_RS08375: BWI76_RS08375 - pyroglutamyl-peptidase I, at 1,772,181 to 1,772,825
_RS08375
BWI76_RS08380: BWI76_RS08380 - endonuclease VIII, at 1,772,841 to 1,773,632
_RS08380
Position (kb) 1771
1772
1773 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1770.217 kb on - strand, within BWI76_RS08360 at 1770.298 kb on + strand, within BWI76_RS08360 at 1770.711 kb on + strand, within BWI76_RS08365 at 1771.143 kb on + strand at 1771.145 kb on - strand at 1771.151 kb on - strand at 1771.234 kb on - strand at 1771.286 kb on + strand, within BWI76_RS08370 at 1771.330 kb on + strand, within BWI76_RS08370 at 1772.016 kb on + strand, within BWI76_RS08370 at 1772.108 kb on + strand at 1772.116 kb on + strand at 1772.627 kb on - strand, within BWI76_RS08375 at 1772.633 kb on + strand, within BWI76_RS08375 at 1772.904 kb on + strand at 1772.912 kb on - strand at 1773.069 kb on + strand, within BWI76_RS08380
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 1,770,217 - BWI76_RS08360 0.77 -2.7 1,770,298 + BWI76_RS08360 0.87 -0.8 1,770,711 + BWI76_RS08365 0.35 +1.2 1,771,143 + +0.1 1,771,145 - +2.3 1,771,151 - -2.8 1,771,234 - -0.5 1,771,286 + BWI76_RS08370 0.11 -2.1 1,771,330 + BWI76_RS08370 0.15 -1.8 1,772,016 + BWI76_RS08370 0.84 -0.2 1,772,108 + -0.5 1,772,116 + -0.0 1,772,627 - BWI76_RS08375 0.69 +1.0 1,772,633 + BWI76_RS08375 0.70 -3.6 1,772,904 + +0.5 1,772,912 - +0.3 1,773,069 + BWI76_RS08380 0.29 +3.0
Or see this region's nucleotide sequence