Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07290 and BWI76_RS07295 overlap by 4 nucleotides BWI76_RS07295 and BWI76_RS07300 overlap by 4 nucleotides BWI76_RS07300 and BWI76_RS07305 are separated by 146 nucleotides
BWI76_RS07290: BWI76_RS07290 - PTS beta-glucoside transporter subunit EIIBCA, at 1,533,799 to 1,535,679
_RS07290
BWI76_RS07295: BWI76_RS07295 - 6-phospho-beta-glucosidase, at 1,535,676 to 1,537,100
_RS07295
BWI76_RS07300: BWI76_RS07300 - antitermination protein BlgG, at 1,537,097 to 1,537,936
_RS07300
BWI76_RS07305: BWI76_RS07305 - sucrose operon repressor, at 1,538,083 to 1,539,087
_RS07305
Position (kb) 1535
1536
1537
1538 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1534.934 kb on - strand, within BWI76_RS07290 at 1535.111 kb on + strand, within BWI76_RS07290 at 1535.111 kb on + strand, within BWI76_RS07290 at 1535.111 kb on + strand, within BWI76_RS07290 at 1535.119 kb on - strand, within BWI76_RS07290 at 1535.119 kb on - strand, within BWI76_RS07290 at 1535.119 kb on - strand, within BWI76_RS07290 at 1535.343 kb on + strand, within BWI76_RS07290 at 1535.392 kb on - strand, within BWI76_RS07290 at 1535.408 kb on + strand, within BWI76_RS07290 at 1535.416 kb on - strand, within BWI76_RS07290 at 1535.449 kb on - strand, within BWI76_RS07290 at 1535.552 kb on - strand at 1535.590 kb on + strand at 1535.600 kb on + strand at 1535.710 kb on + strand at 1535.738 kb on - strand at 1535.752 kb on - strand at 1535.864 kb on - strand, within BWI76_RS07295 at 1535.888 kb on + strand, within BWI76_RS07295 at 1536.067 kb on - strand, within BWI76_RS07295 at 1536.102 kb on - strand, within BWI76_RS07295 at 1536.109 kb on + strand, within BWI76_RS07295 at 1536.284 kb on + strand, within BWI76_RS07295 at 1536.373 kb on - strand, within BWI76_RS07295 at 1536.423 kb on - strand, within BWI76_RS07295 at 1536.664 kb on - strand, within BWI76_RS07295 at 1536.788 kb on + strand, within BWI76_RS07295 at 1536.811 kb on - strand, within BWI76_RS07295 at 1536.941 kb on + strand, within BWI76_RS07295 at 1536.949 kb on - strand, within BWI76_RS07295 at 1537.184 kb on + strand, within BWI76_RS07300 at 1537.280 kb on - strand, within BWI76_RS07300 at 1537.280 kb on - strand, within BWI76_RS07300 at 1537.377 kb on + strand, within BWI76_RS07300 at 1537.588 kb on + strand, within BWI76_RS07300 at 1537.658 kb on + strand, within BWI76_RS07300 at 1537.665 kb on + strand, within BWI76_RS07300 at 1537.732 kb on + strand, within BWI76_RS07300 at 1537.863 kb on + strand at 1537.899 kb on + strand at 1537.949 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 1,534,934 - BWI76_RS07290 0.60 -1.1 1,535,111 + BWI76_RS07290 0.70 +0.2 1,535,111 + BWI76_RS07290 0.70 +0.0 1,535,111 + BWI76_RS07290 0.70 +0.5 1,535,119 - BWI76_RS07290 0.70 -1.3 1,535,119 - BWI76_RS07290 0.70 +0.8 1,535,119 - BWI76_RS07290 0.70 -1.4 1,535,343 + BWI76_RS07290 0.82 +0.2 1,535,392 - BWI76_RS07290 0.85 +2.4 1,535,408 + BWI76_RS07290 0.86 +0.9 1,535,416 - BWI76_RS07290 0.86 +0.3 1,535,449 - BWI76_RS07290 0.88 +1.0 1,535,552 - +1.7 1,535,590 + -0.2 1,535,600 + +0.5 1,535,710 + +0.5 1,535,738 - +0.4 1,535,752 - -3.5 1,535,864 - BWI76_RS07295 0.13 +0.5 1,535,888 + BWI76_RS07295 0.15 +0.7 1,536,067 - BWI76_RS07295 0.27 -0.2 1,536,102 - BWI76_RS07295 0.30 +0.9 1,536,109 + BWI76_RS07295 0.30 -2.2 1,536,284 + BWI76_RS07295 0.43 +2.4 1,536,373 - BWI76_RS07295 0.49 +1.6 1,536,423 - BWI76_RS07295 0.52 -2.4 1,536,664 - BWI76_RS07295 0.69 -0.6 1,536,788 + BWI76_RS07295 0.78 -2.0 1,536,811 - BWI76_RS07295 0.80 +3.1 1,536,941 + BWI76_RS07295 0.89 +2.6 1,536,949 - BWI76_RS07295 0.89 -3.9 1,537,184 + BWI76_RS07300 0.10 -0.5 1,537,280 - BWI76_RS07300 0.22 +0.6 1,537,280 - BWI76_RS07300 0.22 -1.8 1,537,377 + BWI76_RS07300 0.33 +0.4 1,537,588 + BWI76_RS07300 0.58 -0.0 1,537,658 + BWI76_RS07300 0.67 +0.7 1,537,665 + BWI76_RS07300 0.68 -0.4 1,537,732 + BWI76_RS07300 0.76 +2.0 1,537,863 + +0.7 1,537,899 + -1.1 1,537,949 - -0.8
Or see this region's nucleotide sequence