Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06405

Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06395 and BWI76_RS06400 are separated by 58 nucleotidesBWI76_RS06400 and BWI76_RS06405 overlap by 38 nucleotidesBWI76_RS06405 and BWI76_RS06410 are separated by 142 nucleotidesBWI76_RS06410 and BWI76_RS06415 are separated by 114 nucleotides BWI76_RS06395: BWI76_RS06395 - tRNA 4-thiouridine(8) synthase ThiI, at 1,343,831 to 1,345,279 _RS06395 BWI76_RS06400: BWI76_RS06400 - oxidative-stress-resistance chaperone, at 1,345,338 to 1,345,928 _RS06400 BWI76_RS06405: BWI76_RS06405 - 2-dehydropantoate 2-reductase, at 1,345,891 to 1,346,802 _RS06405 BWI76_RS06410: BWI76_RS06410 - YajQ family cyclic di-GMP-binding protein, at 1,346,945 to 1,347,436 _RS06410 BWI76_RS06415: BWI76_RS06415 - MFS transporter, at 1,347,551 to 1,348,915 _RS06415 Position (kb) 1345 1346 1347Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1345.113 kb on - strand, within BWI76_RS06395at 1345.420 kb on - strand, within BWI76_RS06400at 1345.780 kb on - strandat 1345.835 kb on + strand, within BWI76_RS06400at 1346.458 kb on - strand, within BWI76_RS06405at 1346.480 kb on - strand, within BWI76_RS06405at 1346.537 kb on - strand, within BWI76_RS06405at 1346.719 kb on + strandat 1346.727 kb on - strandat 1347.242 kb on - strand, within BWI76_RS06410at 1347.646 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
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1,345,113 - BWI76_RS06395 0.88 -1.7
1,345,420 - BWI76_RS06400 0.14 -0.7
1,345,780 - +0.3
1,345,835 + BWI76_RS06400 0.84 +0.3
1,346,458 - BWI76_RS06405 0.62 +1.3
1,346,480 - BWI76_RS06405 0.65 -3.5
1,346,537 - BWI76_RS06405 0.71 -1.3
1,346,719 + -4.3
1,346,727 - +0.4
1,347,242 - BWI76_RS06410 0.60 -0.2
1,347,646 - +1.1

Or see this region's nucleotide sequence