Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05565 and BWI76_RS05570 are separated by 2 nucleotides BWI76_RS05570 and BWI76_RS05575 overlap by 4 nucleotides
BWI76_RS05565: BWI76_RS05565 - beta-galactosidase, at 1,165,171 to 1,166,589
_RS05565
BWI76_RS05570: BWI76_RS05570 - hypothetical protein, at 1,166,592 to 1,167,002
_RS05570
BWI76_RS05575: BWI76_RS05575 - group 1 glycosyl transferase, at 1,166,999 to 1,168,114
_RS05575
Position (kb) 1166
1167
1168 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1165.614 kb on + strand, within BWI76_RS05565 at 1165.616 kb on + strand, within BWI76_RS05565 at 1165.616 kb on + strand, within BWI76_RS05565 at 1165.652 kb on + strand, within BWI76_RS05565 at 1165.659 kb on - strand, within BWI76_RS05565 at 1165.670 kb on + strand, within BWI76_RS05565 at 1165.678 kb on - strand, within BWI76_RS05565 at 1165.849 kb on - strand, within BWI76_RS05565 at 1165.880 kb on + strand, within BWI76_RS05565 at 1165.880 kb on + strand, within BWI76_RS05565 at 1165.880 kb on + strand, within BWI76_RS05565 at 1165.937 kb on + strand, within BWI76_RS05565 at 1166.292 kb on - strand, within BWI76_RS05565 at 1166.406 kb on - strand, within BWI76_RS05565 at 1166.526 kb on + strand at 1166.557 kb on - strand at 1166.782 kb on + strand, within BWI76_RS05570 at 1166.981 kb on + strand at 1167.207 kb on - strand, within BWI76_RS05575 at 1167.224 kb on + strand, within BWI76_RS05575 at 1167.225 kb on + strand, within BWI76_RS05575 at 1167.250 kb on - strand, within BWI76_RS05575 at 1167.316 kb on + strand, within BWI76_RS05575 at 1167.591 kb on + strand, within BWI76_RS05575 at 1167.599 kb on - strand, within BWI76_RS05575 at 1167.814 kb on + strand, within BWI76_RS05575 at 1167.814 kb on + strand, within BWI76_RS05575 at 1167.819 kb on + strand, within BWI76_RS05575 at 1167.822 kb on - strand, within BWI76_RS05575 at 1167.952 kb on - strand, within BWI76_RS05575
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 1,165,614 + BWI76_RS05565 0.31 -3.2 1,165,616 + BWI76_RS05565 0.31 -0.2 1,165,616 + BWI76_RS05565 0.31 -1.0 1,165,652 + BWI76_RS05565 0.34 -0.3 1,165,659 - BWI76_RS05565 0.34 +0.7 1,165,670 + BWI76_RS05565 0.35 -1.0 1,165,678 - BWI76_RS05565 0.36 -0.0 1,165,849 - BWI76_RS05565 0.48 -0.2 1,165,880 + BWI76_RS05565 0.50 +2.0 1,165,880 + BWI76_RS05565 0.50 +0.4 1,165,880 + BWI76_RS05565 0.50 +0.3 1,165,937 + BWI76_RS05565 0.54 -3.9 1,166,292 - BWI76_RS05565 0.79 +2.2 1,166,406 - BWI76_RS05565 0.87 +0.9 1,166,526 + -0.9 1,166,557 - +0.9 1,166,782 + BWI76_RS05570 0.46 -2.9 1,166,981 + -0.5 1,167,207 - BWI76_RS05575 0.19 +1.4 1,167,224 + BWI76_RS05575 0.20 -0.2 1,167,225 + BWI76_RS05575 0.20 +0.1 1,167,250 - BWI76_RS05575 0.22 +0.1 1,167,316 + BWI76_RS05575 0.28 +2.1 1,167,591 + BWI76_RS05575 0.53 +0.0 1,167,599 - BWI76_RS05575 0.54 -0.9 1,167,814 + BWI76_RS05575 0.73 +0.5 1,167,814 + BWI76_RS05575 0.73 +1.2 1,167,819 + BWI76_RS05575 0.73 +0.5 1,167,822 - BWI76_RS05575 0.74 +0.3 1,167,952 - BWI76_RS05575 0.85 -0.2
Or see this region's nucleotide sequence