Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04540 and BWI76_RS04545 are separated by 30 nucleotides BWI76_RS04545 and BWI76_RS04550 overlap by 11 nucleotides BWI76_RS04550 and BWI76_RS04555 are separated by 323 nucleotides
BWI76_RS04540: BWI76_RS04540 - molecular chaperone DjlA, at 940,471 to 941,298
_RS04540
BWI76_RS04545: BWI76_RS04545 - two-component response regulator for citrate, at 941,329 to 942,027
_RS04545
BWI76_RS04550: BWI76_RS04550 - two-component sensor kinase, at 942,017 to 943,642
_RS04550
BWI76_RS04555: BWI76_RS04555 - citrate/acetate antiporter, at 943,966 to 945,321
_RS04555
Position (kb)
942
943
944 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 941.159 kb on + strand, within BWI76_RS04540 at 941.167 kb on - strand, within BWI76_RS04540 at 941.174 kb on - strand, within BWI76_RS04540 at 941.187 kb on - strand, within BWI76_RS04540 at 941.189 kb on - strand, within BWI76_RS04540 at 941.264 kb on + strand at 941.422 kb on + strand, within BWI76_RS04545 at 941.432 kb on - strand, within BWI76_RS04545 at 941.586 kb on - strand, within BWI76_RS04545 at 941.653 kb on + strand, within BWI76_RS04545 at 941.693 kb on + strand, within BWI76_RS04545 at 941.742 kb on + strand, within BWI76_RS04545 at 941.829 kb on + strand, within BWI76_RS04545 at 942.066 kb on + strand at 942.141 kb on - strand at 942.424 kb on + strand, within BWI76_RS04550 at 942.439 kb on - strand, within BWI76_RS04550 at 942.473 kb on - strand, within BWI76_RS04550 at 942.516 kb on + strand, within BWI76_RS04550 at 942.593 kb on - strand, within BWI76_RS04550 at 942.606 kb on - strand, within BWI76_RS04550 at 942.628 kb on + strand, within BWI76_RS04550 at 942.636 kb on - strand, within BWI76_RS04550 at 942.662 kb on + strand, within BWI76_RS04550 at 942.671 kb on - strand, within BWI76_RS04550 at 942.719 kb on + strand, within BWI76_RS04550 at 943.022 kb on - strand, within BWI76_RS04550 at 943.082 kb on + strand, within BWI76_RS04550 at 943.243 kb on + strand, within BWI76_RS04550 at 943.280 kb on + strand, within BWI76_RS04550 at 943.331 kb on + strand, within BWI76_RS04550 at 943.336 kb on + strand, within BWI76_RS04550 at 943.360 kb on + strand, within BWI76_RS04550 at 943.406 kb on - strand, within BWI76_RS04550 at 943.455 kb on + strand, within BWI76_RS04550 at 943.537 kb on + strand at 943.537 kb on + strand at 943.537 kb on + strand at 943.566 kb on + strand at 943.566 kb on + strand at 943.566 kb on + strand at 943.566 kb on + strand at 943.566 kb on + strand at 943.567 kb on - strand at 943.574 kb on - strand at 943.574 kb on - strand at 943.574 kb on - strand at 943.574 kb on - strand at 943.574 kb on - strand at 943.618 kb on + strand at 943.625 kb on - strand at 943.645 kb on + strand at 943.645 kb on + strand at 943.645 kb on + strand at 943.645 kb on + strand at 943.645 kb on + strand at 943.645 kb on + strand at 943.653 kb on - strand at 943.653 kb on - strand at 943.653 kb on - strand at 943.653 kb on - strand at 943.653 kb on - strand at 943.657 kb on + strand at 943.657 kb on + strand at 943.657 kb on + strand at 943.657 kb on + strand at 943.657 kb on + strand at 943.657 kb on + strand at 943.665 kb on - strand at 943.665 kb on - strand at 943.665 kb on - strand at 943.665 kb on - strand at 943.665 kb on - strand at 943.703 kb on + strand at 943.703 kb on + strand at 943.708 kb on + strand at 943.710 kb on + strand at 943.713 kb on + strand at 943.716 kb on - strand at 943.718 kb on + strand at 943.733 kb on + strand at 943.733 kb on + strand at 943.733 kb on + strand at 943.734 kb on + strand at 943.741 kb on - strand at 943.741 kb on - strand at 943.741 kb on - strand at 943.748 kb on + strand at 943.774 kb on - strand at 943.776 kb on + strand at 943.823 kb on + strand at 943.862 kb on - strand at 943.881 kb on - strand at 943.902 kb on + strand at 943.905 kb on + strand at 943.905 kb on + strand at 943.907 kb on + strand at 943.907 kb on + strand at 943.907 kb on + strand at 943.907 kb on + strand at 943.907 kb on + strand at 943.907 kb on + strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.915 kb on - strand at 943.917 kb on + strand at 943.917 kb on + strand at 943.917 kb on + strand at 943.925 kb on - strand at 943.925 kb on - strand at 943.926 kb on + strand at 943.926 kb on + strand at 943.926 kb on + strand at 943.934 kb on - strand at 943.934 kb on - strand at 943.959 kb on - strand at 943.973 kb on + strand at 943.973 kb on + strand at 943.973 kb on + strand at 943.988 kb on - strand at 943.990 kb on - strand at 944.042 kb on + strand at 944.060 kb on + strand at 944.072 kb on + strand at 944.180 kb on + strand, within BWI76_RS04555 at 944.240 kb on - strand, within BWI76_RS04555 at 944.240 kb on - strand, within BWI76_RS04555 at 944.250 kb on - strand, within BWI76_RS04555 at 944.267 kb on + strand, within BWI76_RS04555 at 944.288 kb on + strand, within BWI76_RS04555 at 944.390 kb on + strand, within BWI76_RS04555 at 944.399 kb on + strand, within BWI76_RS04555 at 944.409 kb on + strand, within BWI76_RS04555 at 944.474 kb on + strand, within BWI76_RS04555
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 941,159 + BWI76_RS04540 0.83 +0.5 941,167 - BWI76_RS04540 0.84 -0.8 941,174 - BWI76_RS04540 0.85 -0.6 941,187 - BWI76_RS04540 0.86 +0.1 941,189 - BWI76_RS04540 0.87 -0.1 941,264 + -2.4 941,422 + BWI76_RS04545 0.13 -3.4 941,432 - BWI76_RS04545 0.15 -1.0 941,586 - BWI76_RS04545 0.37 +0.5 941,653 + BWI76_RS04545 0.46 +1.0 941,693 + BWI76_RS04545 0.52 -1.0 941,742 + BWI76_RS04545 0.59 -0.9 941,829 + BWI76_RS04545 0.72 +0.3 942,066 + -1.2 942,141 - -4.3 942,424 + BWI76_RS04550 0.25 -0.2 942,439 - BWI76_RS04550 0.26 +0.9 942,473 - BWI76_RS04550 0.28 +0.8 942,516 + BWI76_RS04550 0.31 -2.2 942,593 - BWI76_RS04550 0.35 -0.2 942,606 - BWI76_RS04550 0.36 +0.7 942,628 + BWI76_RS04550 0.38 +1.2 942,636 - BWI76_RS04550 0.38 -1.2 942,662 + BWI76_RS04550 0.40 +0.1 942,671 - BWI76_RS04550 0.40 +3.3 942,719 + BWI76_RS04550 0.43 +0.5 943,022 - BWI76_RS04550 0.62 -0.2 943,082 + BWI76_RS04550 0.65 +1.4 943,243 + BWI76_RS04550 0.75 -0.5 943,280 + BWI76_RS04550 0.78 +0.1 943,331 + BWI76_RS04550 0.81 +0.1 943,336 + BWI76_RS04550 0.81 +1.4 943,360 + BWI76_RS04550 0.83 +0.1 943,406 - BWI76_RS04550 0.85 +0.2 943,455 + BWI76_RS04550 0.88 +0.8 943,537 + +0.3 943,537 + +0.2 943,537 + +0.7 943,566 + -0.3 943,566 + -0.2 943,566 + -1.2 943,566 + +0.3 943,566 + -0.2 943,567 - -0.4 943,574 - -0.6 943,574 - -0.6 943,574 - -0.9 943,574 - -0.1 943,574 - -0.8 943,618 + -0.6 943,625 - -0.4 943,645 + +1.7 943,645 + -0.5 943,645 + -2.8 943,645 + -2.4 943,645 + +0.3 943,645 + +1.0 943,653 - -1.1 943,653 - -1.6 943,653 - +0.4 943,653 - +0.2 943,653 - -1.2 943,657 + -1.2 943,657 + -2.6 943,657 + -1.8 943,657 + -1.0 943,657 + +1.0 943,657 + +0.5 943,665 - -1.6 943,665 - -1.5 943,665 - -0.0 943,665 - +1.2 943,665 - +0.3 943,703 + -0.2 943,703 + -1.0 943,708 + +0.0 943,710 + +0.4 943,713 + -0.2 943,716 - -1.2 943,718 + -1.8 943,733 + -2.9 943,733 + -2.9 943,733 + -0.2 943,734 + +0.4 943,741 - +0.9 943,741 - +1.6 943,741 - +1.4 943,748 + +0.0 943,774 - +1.1 943,776 + -1.9 943,823 + +0.9 943,862 - +1.0 943,881 - -0.6 943,902 + -1.8 943,905 + -0.3 943,905 + -0.8 943,907 + +0.8 943,907 + -0.3 943,907 + -0.1 943,907 + -1.0 943,907 + +0.5 943,907 + -0.1 943,915 - -0.0 943,915 - +1.0 943,915 - -0.6 943,915 - -1.0 943,915 - -2.8 943,915 - -3.6 943,915 - -1.6 943,917 + +0.5 943,917 + -2.9 943,917 + -0.5 943,925 - +0.3 943,925 - -2.2 943,926 + +1.2 943,926 + +0.3 943,926 + -0.7 943,934 - -3.0 943,934 - -2.6 943,959 - -1.1 943,973 + -0.6 943,973 + -0.4 943,973 + -0.2 943,988 - +1.3 943,990 - -0.4 944,042 + -2.5 944,060 + -0.9 944,072 + +1.4 944,180 + BWI76_RS04555 0.16 -0.3 944,240 - BWI76_RS04555 0.20 -1.6 944,240 - BWI76_RS04555 0.20 +1.2 944,250 - BWI76_RS04555 0.21 -0.9 944,267 + BWI76_RS04555 0.22 +0.8 944,288 + BWI76_RS04555 0.24 +0.7 944,390 + BWI76_RS04555 0.31 +0.0 944,399 + BWI76_RS04555 0.32 -0.5 944,409 + BWI76_RS04555 0.33 +0.1 944,474 + BWI76_RS04555 0.37 -1.8
Or see this region's nucleotide sequence