Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03915 and BWI76_RS03920 are separated by 70 nucleotides BWI76_RS03920 and BWI76_RS03925 are separated by 32 nucleotides BWI76_RS03925 and BWI76_RS03930 are separated by 3 nucleotides
BWI76_RS03915: BWI76_RS03915 - MurR/RpiR family transcriptional regulator, at 809,174 to 810,037
_RS03915
BWI76_RS03920: BWI76_RS03920 - N-acetyltransferase, at 810,108 to 810,641
_RS03920
BWI76_RS03925: BWI76_RS03925 - N-acetyltransferase GCN5, at 810,674 to 811,510
_RS03925
BWI76_RS03930: BWI76_RS03930 - MFS transporter, at 811,514 to 812,875
_RS03930
Position (kb) 810
811
812 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 809.718 kb on + strand, within BWI76_RS03915 at 809.768 kb on - strand, within BWI76_RS03915 at 809.998 kb on + strand at 810.120 kb on + strand at 810.158 kb on - strand at 810.197 kb on + strand, within BWI76_RS03920 at 810.349 kb on - strand, within BWI76_RS03920 at 810.421 kb on - strand, within BWI76_RS03920 at 810.649 kb on + strand at 810.669 kb on + strand at 810.687 kb on - strand at 810.723 kb on + strand at 810.810 kb on + strand, within BWI76_RS03925 at 811.013 kb on - strand, within BWI76_RS03925 at 811.067 kb on - strand, within BWI76_RS03925 at 811.141 kb on - strand, within BWI76_RS03925 at 811.142 kb on - strand, within BWI76_RS03925 at 811.274 kb on + strand, within BWI76_RS03925 at 811.557 kb on + strand at 811.654 kb on + strand, within BWI76_RS03930 at 811.693 kb on - strand, within BWI76_RS03930 at 811.693 kb on - strand, within BWI76_RS03930 at 811.695 kb on + strand, within BWI76_RS03930 at 811.714 kb on + strand, within BWI76_RS03930 at 811.770 kb on + strand, within BWI76_RS03930 at 811.775 kb on + strand, within BWI76_RS03930 at 811.775 kb on + strand, within BWI76_RS03930 at 811.845 kb on - strand at 811.851 kb on + strand, within BWI76_RS03930 at 811.851 kb on + strand, within BWI76_RS03930 at 812.017 kb on - strand, within BWI76_RS03930 at 812.054 kb on - strand at 812.059 kb on + strand, within BWI76_RS03930 at 812.212 kb on + strand, within BWI76_RS03930 at 812.351 kb on - strand, within BWI76_RS03930 at 812.358 kb on + strand, within BWI76_RS03930 at 812.366 kb on - strand, within BWI76_RS03930 at 812.366 kb on - strand, within BWI76_RS03930 at 812.432 kb on - strand, within BWI76_RS03930 at 812.509 kb on + strand, within BWI76_RS03930
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 809,718 + BWI76_RS03915 0.63 -0.2 809,768 - BWI76_RS03915 0.69 -0.3 809,998 + +0.0 810,120 + +0.6 810,158 - +0.0 810,197 + BWI76_RS03920 0.17 -0.3 810,349 - BWI76_RS03920 0.45 -3.5 810,421 - BWI76_RS03920 0.59 +1.4 810,649 + +0.7 810,669 + +0.1 810,687 - -0.4 810,723 + +0.4 810,810 + BWI76_RS03925 0.16 -0.3 811,013 - BWI76_RS03925 0.41 -1.0 811,067 - BWI76_RS03925 0.47 -1.6 811,141 - BWI76_RS03925 0.56 -1.0 811,142 - BWI76_RS03925 0.56 -3.0 811,274 + BWI76_RS03925 0.72 -1.3 811,557 + +1.4 811,654 + BWI76_RS03930 0.10 -1.8 811,693 - BWI76_RS03930 0.13 +0.1 811,693 - BWI76_RS03930 0.13 +0.2 811,695 + BWI76_RS03930 0.13 +0.2 811,714 + BWI76_RS03930 0.15 +0.3 811,770 + BWI76_RS03930 0.19 -1.1 811,775 + BWI76_RS03930 0.19 -2.2 811,775 + BWI76_RS03930 0.19 -0.5 811,845 - +1.4 811,851 + BWI76_RS03930 0.25 -1.4 811,851 + BWI76_RS03930 0.25 -0.6 812,017 - BWI76_RS03930 0.37 -0.2 812,054 - +1.4 812,059 + BWI76_RS03930 0.40 +1.4 812,212 + BWI76_RS03930 0.51 +0.3 812,351 - BWI76_RS03930 0.61 -0.2 812,358 + BWI76_RS03930 0.62 +0.3 812,366 - BWI76_RS03930 0.63 +0.7 812,366 - BWI76_RS03930 0.63 -2.5 812,432 - BWI76_RS03930 0.67 -0.6 812,509 + BWI76_RS03930 0.73 +1.0
Or see this region's nucleotide sequence