Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03140 and BWI76_RS03145 are separated by 233 nucleotides BWI76_RS03145 and BWI76_RS03150 are separated by 164 nucleotides BWI76_RS03150 and BWI76_RS03155 are separated by 126 nucleotides
BWI76_RS03140: BWI76_RS03140 - alcohol dehydrogenase, at 647,173 to 648,192
_RS03140
BWI76_RS03145: BWI76_RS03145 - tRNA-Leu, at 648,426 to 648,510
_RS03145
BWI76_RS03150: BWI76_RS03150 - site-specific recombinase, phage integrase family protein, at 648,675 to 649,937
_RS03150
BWI76_RS03155: BWI76_RS03155 - hypothetical protein, at 650,064 to 650,966
_RS03155
Position (kb) 648
649
650 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 647.891 kb on + strand, within BWI76_RS03140 at 647.899 kb on - strand, within BWI76_RS03140 at 647.945 kb on + strand, within BWI76_RS03140 at 647.954 kb on + strand, within BWI76_RS03140 at 647.954 kb on + strand, within BWI76_RS03140 at 647.992 kb on + strand, within BWI76_RS03140 at 647.992 kb on + strand, within BWI76_RS03140 at 648.090 kb on - strand, within BWI76_RS03140 at 648.195 kb on - strand at 648.217 kb on + strand at 648.237 kb on - strand at 648.241 kb on + strand at 648.285 kb on - strand at 648.288 kb on - strand at 648.309 kb on - strand at 648.685 kb on + strand at 648.691 kb on + strand at 648.695 kb on + strand at 648.703 kb on - strand at 648.710 kb on + strand at 648.841 kb on + strand, within BWI76_RS03150 at 648.867 kb on - strand, within BWI76_RS03150 at 648.869 kb on + strand, within BWI76_RS03150 at 648.877 kb on - strand, within BWI76_RS03150 at 648.877 kb on - strand, within BWI76_RS03150 at 648.906 kb on + strand, within BWI76_RS03150 at 648.906 kb on + strand, within BWI76_RS03150 at 648.913 kb on + strand, within BWI76_RS03150 at 648.921 kb on - strand, within BWI76_RS03150 at 648.963 kb on + strand, within BWI76_RS03150 at 649.056 kb on - strand, within BWI76_RS03150 at 649.097 kb on + strand, within BWI76_RS03150 at 649.097 kb on + strand, within BWI76_RS03150 at 649.097 kb on + strand, within BWI76_RS03150 at 649.112 kb on + strand, within BWI76_RS03150 at 649.151 kb on + strand, within BWI76_RS03150 at 649.159 kb on - strand, within BWI76_RS03150 at 649.206 kb on + strand, within BWI76_RS03150 at 649.214 kb on - strand, within BWI76_RS03150 at 649.225 kb on + strand, within BWI76_RS03150 at 649.225 kb on + strand, within BWI76_RS03150 at 649.233 kb on - strand, within BWI76_RS03150 at 649.233 kb on - strand, within BWI76_RS03150 at 649.248 kb on - strand, within BWI76_RS03150 at 649.277 kb on + strand, within BWI76_RS03150 at 649.287 kb on + strand, within BWI76_RS03150 at 649.295 kb on - strand, within BWI76_RS03150 at 649.329 kb on + strand, within BWI76_RS03150 at 649.363 kb on + strand, within BWI76_RS03150 at 649.371 kb on - strand, within BWI76_RS03150 at 649.489 kb on - strand, within BWI76_RS03150 at 649.500 kb on - strand, within BWI76_RS03150 at 649.500 kb on - strand, within BWI76_RS03150 at 649.519 kb on - strand, within BWI76_RS03150 at 649.523 kb on + strand, within BWI76_RS03150 at 649.577 kb on + strand, within BWI76_RS03150 at 649.585 kb on - strand, within BWI76_RS03150 at 649.585 kb on - strand, within BWI76_RS03150 at 649.619 kb on - strand, within BWI76_RS03150 at 649.691 kb on + strand, within BWI76_RS03150 at 649.704 kb on + strand, within BWI76_RS03150 at 649.706 kb on + strand, within BWI76_RS03150 at 649.706 kb on + strand, within BWI76_RS03150 at 649.711 kb on + strand, within BWI76_RS03150 at 649.714 kb on - strand, within BWI76_RS03150 at 649.714 kb on - strand, within BWI76_RS03150 at 649.723 kb on + strand, within BWI76_RS03150 at 649.743 kb on + strand, within BWI76_RS03150 at 649.743 kb on + strand, within BWI76_RS03150 at 649.743 kb on + strand, within BWI76_RS03150 at 649.746 kb on - strand, within BWI76_RS03150 at 649.748 kb on + strand, within BWI76_RS03150 at 649.751 kb on - strand, within BWI76_RS03150 at 649.751 kb on - strand, within BWI76_RS03150 at 649.751 kb on - strand at 649.783 kb on + strand, within BWI76_RS03150 at 649.783 kb on + strand, within BWI76_RS03150 at 649.798 kb on - strand, within BWI76_RS03150 at 649.853 kb on - strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.854 kb on + strand at 649.855 kb on - strand at 649.855 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.862 kb on - strand at 649.867 kb on - strand at 649.867 kb on - strand at 649.867 kb on - strand at 649.873 kb on + strand at 649.881 kb on - strand at 649.888 kb on + strand at 649.903 kb on - strand at 649.905 kb on + strand at 649.905 kb on + strand at 649.905 kb on + strand at 649.905 kb on - strand at 649.905 kb on - strand at 649.906 kb on - strand at 649.906 kb on - strand at 649.913 kb on - strand at 649.913 kb on - strand at 649.913 kb on - strand at 649.913 kb on - strand at 649.913 kb on - strand at 649.973 kb on + strand at 649.973 kb on + strand at 649.973 kb on + strand at 649.983 kb on + strand at 649.983 kb on + strand at 649.991 kb on - strand at 650.061 kb on - strand at 650.117 kb on - strand at 650.121 kb on + strand at 650.132 kb on + strand at 650.132 kb on + strand at 650.140 kb on - strand at 650.163 kb on + strand, within BWI76_RS03155 at 650.190 kb on + strand at 650.190 kb on + strand, within BWI76_RS03155 at 650.190 kb on + strand at 650.194 kb on + strand, within BWI76_RS03155 at 650.198 kb on + strand, within BWI76_RS03155 at 650.198 kb on + strand, within BWI76_RS03155 at 650.198 kb on - strand, within BWI76_RS03155 at 650.200 kb on + strand, within BWI76_RS03155 at 650.208 kb on - strand, within BWI76_RS03155 at 650.209 kb on - strand, within BWI76_RS03155 at 650.213 kb on + strand, within BWI76_RS03155 at 650.213 kb on + strand, within BWI76_RS03155 at 650.213 kb on + strand, within BWI76_RS03155 at 650.215 kb on + strand, within BWI76_RS03155 at 650.215 kb on + strand, within BWI76_RS03155 at 650.215 kb on + strand, within BWI76_RS03155 at 650.221 kb on + strand, within BWI76_RS03155 at 650.221 kb on - strand, within BWI76_RS03155 at 650.221 kb on - strand, within BWI76_RS03155 at 650.221 kb on - strand, within BWI76_RS03155 at 650.221 kb on - strand, within BWI76_RS03155 at 650.223 kb on - strand, within BWI76_RS03155 at 650.228 kb on + strand, within BWI76_RS03155 at 650.236 kb on + strand, within BWI76_RS03155 at 650.236 kb on + strand, within BWI76_RS03155 at 650.236 kb on + strand, within BWI76_RS03155 at 650.236 kb on + strand, within BWI76_RS03155 at 650.236 kb on + strand, within BWI76_RS03155 at 650.244 kb on - strand, within BWI76_RS03155 at 650.244 kb on - strand, within BWI76_RS03155 at 650.244 kb on - strand, within BWI76_RS03155 at 650.244 kb on - strand, within BWI76_RS03155 at 650.244 kb on - strand, within BWI76_RS03155 at 650.246 kb on + strand, within BWI76_RS03155 at 650.246 kb on + strand at 650.246 kb on + strand, within BWI76_RS03155 at 650.246 kb on + strand, within BWI76_RS03155 at 650.246 kb on + strand, within BWI76_RS03155 at 650.246 kb on + strand, within BWI76_RS03155 at 650.246 kb on + strand, within BWI76_RS03155 at 650.246 kb on + strand, within BWI76_RS03155 at 650.249 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.254 kb on - strand, within BWI76_RS03155 at 650.259 kb on + strand, within BWI76_RS03155 at 650.262 kb on - strand, within BWI76_RS03155 at 650.264 kb on - strand, within BWI76_RS03155 at 650.289 kb on - strand, within BWI76_RS03155 at 650.289 kb on - strand, within BWI76_RS03155 at 650.541 kb on + strand at 650.541 kb on + strand, within BWI76_RS03155 at 650.542 kb on + strand, within BWI76_RS03155 at 650.542 kb on + strand, within BWI76_RS03155 at 650.549 kb on - strand, within BWI76_RS03155 at 650.550 kb on - strand, within BWI76_RS03155 at 650.550 kb on - strand, within BWI76_RS03155 at 650.557 kb on + strand, within BWI76_RS03155 at 650.560 kb on - strand, within BWI76_RS03155 at 650.565 kb on - strand, within BWI76_RS03155 at 650.807 kb on - strand, within BWI76_RS03155 at 650.807 kb on - strand, within BWI76_RS03155 at 650.877 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 647,891 + BWI76_RS03140 0.70 -0.9 647,899 - BWI76_RS03140 0.71 -0.0 647,945 + BWI76_RS03140 0.76 +1.5 647,954 + BWI76_RS03140 0.77 -1.1 647,954 + BWI76_RS03140 0.77 -0.0 647,992 + BWI76_RS03140 0.80 +1.5 647,992 + BWI76_RS03140 0.80 -1.4 648,090 - BWI76_RS03140 0.90 -0.2 648,195 - +0.9 648,217 + -1.3 648,237 - +0.1 648,241 + -0.9 648,285 - -1.1 648,288 - +0.8 648,309 - +1.1 648,685 + +0.6 648,691 + -0.5 648,695 + +0.5 648,703 - +0.1 648,710 + +1.0 648,841 + BWI76_RS03150 0.13 -0.5 648,867 - BWI76_RS03150 0.15 -0.8 648,869 + BWI76_RS03150 0.15 +0.6 648,877 - BWI76_RS03150 0.16 +0.2 648,877 - BWI76_RS03150 0.16 -3.2 648,906 + BWI76_RS03150 0.18 -2.5 648,906 + BWI76_RS03150 0.18 +0.5 648,913 + BWI76_RS03150 0.19 +0.5 648,921 - BWI76_RS03150 0.19 -3.8 648,963 + BWI76_RS03150 0.23 -0.8 649,056 - BWI76_RS03150 0.30 +1.5 649,097 + BWI76_RS03150 0.33 -2.8 649,097 + BWI76_RS03150 0.33 -0.8 649,097 + BWI76_RS03150 0.33 -1.1 649,112 + BWI76_RS03150 0.35 +1.5 649,151 + BWI76_RS03150 0.38 +1.5 649,159 - BWI76_RS03150 0.38 +0.4 649,206 + BWI76_RS03150 0.42 -1.9 649,214 - BWI76_RS03150 0.43 +1.0 649,225 + BWI76_RS03150 0.44 -2.0 649,225 + BWI76_RS03150 0.44 -0.8 649,233 - BWI76_RS03150 0.44 +0.2 649,233 - BWI76_RS03150 0.44 +1.5 649,248 - BWI76_RS03150 0.45 +0.6 649,277 + BWI76_RS03150 0.48 -0.9 649,287 + BWI76_RS03150 0.48 +0.9 649,295 - BWI76_RS03150 0.49 -1.3 649,329 + BWI76_RS03150 0.52 -0.0 649,363 + BWI76_RS03150 0.54 +1.1 649,371 - BWI76_RS03150 0.55 +0.9 649,489 - BWI76_RS03150 0.64 -3.8 649,500 - BWI76_RS03150 0.65 -2.2 649,500 - BWI76_RS03150 0.65 -2.7 649,519 - BWI76_RS03150 0.67 +1.5 649,523 + BWI76_RS03150 0.67 -1.2 649,577 + BWI76_RS03150 0.71 -3.0 649,585 - BWI76_RS03150 0.72 +0.5 649,585 - BWI76_RS03150 0.72 +0.3 649,619 - BWI76_RS03150 0.75 -3.6 649,691 + BWI76_RS03150 0.80 -3.2 649,704 + BWI76_RS03150 0.81 +0.4 649,706 + BWI76_RS03150 0.82 +0.9 649,706 + BWI76_RS03150 0.82 +1.3 649,711 + BWI76_RS03150 0.82 +0.0 649,714 - BWI76_RS03150 0.82 +0.9 649,714 - BWI76_RS03150 0.82 -0.2 649,723 + BWI76_RS03150 0.83 +1.6 649,743 + BWI76_RS03150 0.85 +2.1 649,743 + BWI76_RS03150 0.85 -0.9 649,743 + BWI76_RS03150 0.85 +0.3 649,746 - BWI76_RS03150 0.85 -2.8 649,748 + BWI76_RS03150 0.85 -0.9 649,751 - BWI76_RS03150 0.85 -0.4 649,751 - BWI76_RS03150 0.85 -4.1 649,751 - +3.0 649,783 + BWI76_RS03150 0.88 -1.1 649,783 + BWI76_RS03150 0.88 +0.1 649,798 - BWI76_RS03150 0.89 +1.5 649,853 - -2.3 649,854 + -0.9 649,854 + +0.9 649,854 + -2.7 649,854 + +1.0 649,854 + -0.9 649,854 + -0.6 649,854 + +1.6 649,854 + +1.1 649,854 + +1.5 649,854 + -1.2 649,854 + +0.1 649,854 + +1.4 649,854 + +1.5 649,855 - +1.5 649,855 - -1.9 649,862 - -0.5 649,862 - +1.5 649,862 - +0.5 649,862 - +2.8 649,862 - +0.2 649,862 - +1.7 649,862 - +1.2 649,862 - +0.2 649,862 - +1.3 649,862 - -2.0 649,862 - +0.5 649,867 - +1.5 649,867 - -2.4 649,867 - +0.3 649,873 + -0.5 649,881 - -0.1 649,888 + -0.2 649,903 - +1.5 649,905 + -1.5 649,905 + -1.3 649,905 + +0.5 649,905 - -0.1 649,905 - -0.1 649,906 - +0.8 649,906 - +0.6 649,913 - +1.4 649,913 - +1.1 649,913 - +1.4 649,913 - +0.2 649,913 - -0.2 649,973 + +1.4 649,973 + +0.4 649,973 + -1.1 649,983 + -1.7 649,983 + -0.1 649,991 - -0.1 650,061 - +1.4 650,117 - -0.9 650,121 + -0.1 650,132 + +0.2 650,132 + +1.4 650,140 - -2.3 650,163 + BWI76_RS03155 0.11 +0.7 650,190 + +0.9 650,190 + BWI76_RS03155 0.14 -0.3 650,190 + +5.3 650,194 + BWI76_RS03155 0.14 +0.2 650,198 + BWI76_RS03155 0.15 +0.2 650,198 + BWI76_RS03155 0.15 -1.6 650,198 - BWI76_RS03155 0.15 -0.1 650,200 + BWI76_RS03155 0.15 +0.6 650,208 - BWI76_RS03155 0.16 -0.7 650,209 - BWI76_RS03155 0.16 -0.1 650,213 + BWI76_RS03155 0.17 -0.9 650,213 + BWI76_RS03155 0.17 +0.2 650,213 + BWI76_RS03155 0.17 +1.1 650,215 + BWI76_RS03155 0.17 -2.9 650,215 + BWI76_RS03155 0.17 +0.4 650,215 + BWI76_RS03155 0.17 -0.1 650,221 + BWI76_RS03155 0.17 -0.4 650,221 - BWI76_RS03155 0.17 -0.9 650,221 - BWI76_RS03155 0.17 +2.9 650,221 - BWI76_RS03155 0.17 -0.4 650,221 - BWI76_RS03155 0.17 +0.2 650,223 - BWI76_RS03155 0.18 -0.9 650,228 + BWI76_RS03155 0.18 +1.3 650,236 + BWI76_RS03155 0.19 +0.4 650,236 + BWI76_RS03155 0.19 +0.1 650,236 + BWI76_RS03155 0.19 +1.0 650,236 + BWI76_RS03155 0.19 +0.4 650,236 + BWI76_RS03155 0.19 +0.4 650,244 - BWI76_RS03155 0.20 +0.4 650,244 - BWI76_RS03155 0.20 +0.4 650,244 - BWI76_RS03155 0.20 -3.1 650,244 - BWI76_RS03155 0.20 -2.1 650,244 - BWI76_RS03155 0.20 +0.8 650,246 + BWI76_RS03155 0.20 -3.4 650,246 + +2.4 650,246 + BWI76_RS03155 0.20 -1.9 650,246 + BWI76_RS03155 0.20 +1.2 650,246 + BWI76_RS03155 0.20 +0.4 650,246 + BWI76_RS03155 0.20 +0.8 650,246 + BWI76_RS03155 0.20 +0.4 650,246 + BWI76_RS03155 0.20 +1.9 650,249 - BWI76_RS03155 0.20 -1.4 650,254 - BWI76_RS03155 0.21 -1.6 650,254 - BWI76_RS03155 0.21 +2.7 650,254 - BWI76_RS03155 0.21 +1.3 650,254 - BWI76_RS03155 0.21 +0.8 650,254 - BWI76_RS03155 0.21 -2.1 650,254 - BWI76_RS03155 0.21 -3.5 650,254 - BWI76_RS03155 0.21 -4.1 650,254 - BWI76_RS03155 0.21 -3.2 650,254 - BWI76_RS03155 0.21 +1.0 650,259 + BWI76_RS03155 0.22 -0.1 650,262 - BWI76_RS03155 0.22 +0.1 650,264 - BWI76_RS03155 0.22 -1.1 650,289 - BWI76_RS03155 0.25 +0.2 650,289 - BWI76_RS03155 0.25 -0.9 650,541 + +1.4 650,541 + BWI76_RS03155 0.53 -0.1 650,542 + BWI76_RS03155 0.53 -1.7 650,542 + BWI76_RS03155 0.53 +1.4 650,549 - BWI76_RS03155 0.54 +1.9 650,550 - BWI76_RS03155 0.54 -1.2 650,550 - BWI76_RS03155 0.54 -1.1 650,557 + BWI76_RS03155 0.55 +0.9 650,560 - BWI76_RS03155 0.55 -0.1 650,565 - BWI76_RS03155 0.55 +0.2 650,807 - BWI76_RS03155 0.82 +2.4 650,807 - BWI76_RS03155 0.82 +0.1 650,877 + -1.6
Or see this region's nucleotide sequence