Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03135 and BWI76_RS03140 are separated by 190 nucleotides BWI76_RS03140 and BWI76_RS03145 are separated by 233 nucleotides BWI76_RS03145 and BWI76_RS03150 are separated by 164 nucleotides
BWI76_RS03135: BWI76_RS03135 - sugar ABC transporter permease, at 646,110 to 646,982
_RS03135
BWI76_RS03140: BWI76_RS03140 - alcohol dehydrogenase, at 647,173 to 648,192
_RS03140
BWI76_RS03145: BWI76_RS03145 - tRNA-Leu, at 648,426 to 648,510
_RS03145
BWI76_RS03150: BWI76_RS03150 - site-specific recombinase, phage integrase family protein, at 648,675 to 649,937
_RS03150
Position (kb) 647
648
649 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 646.254 kb on - strand, within BWI76_RS03135 at 646.254 kb on - strand, within BWI76_RS03135 at 646.321 kb on + strand, within BWI76_RS03135 at 646.421 kb on + strand, within BWI76_RS03135 at 646.524 kb on - strand, within BWI76_RS03135 at 646.541 kb on + strand, within BWI76_RS03135 at 646.598 kb on + strand, within BWI76_RS03135 at 646.606 kb on - strand, within BWI76_RS03135 at 646.606 kb on - strand, within BWI76_RS03135 at 646.606 kb on - strand, within BWI76_RS03135 at 646.639 kb on + strand, within BWI76_RS03135 at 646.647 kb on + strand, within BWI76_RS03135 at 646.768 kb on + strand, within BWI76_RS03135 at 646.768 kb on + strand, within BWI76_RS03135 at 646.881 kb on - strand, within BWI76_RS03135 at 646.887 kb on + strand, within BWI76_RS03135 at 647.206 kb on + strand at 647.260 kb on - strand at 647.274 kb on + strand at 647.378 kb on - strand, within BWI76_RS03140 at 647.610 kb on + strand, within BWI76_RS03140 at 647.632 kb on - strand, within BWI76_RS03140 at 647.891 kb on + strand, within BWI76_RS03140 at 647.899 kb on - strand, within BWI76_RS03140 at 647.945 kb on + strand, within BWI76_RS03140 at 647.954 kb on + strand, within BWI76_RS03140 at 647.954 kb on + strand, within BWI76_RS03140 at 647.992 kb on + strand, within BWI76_RS03140 at 647.992 kb on + strand, within BWI76_RS03140 at 648.090 kb on - strand, within BWI76_RS03140 at 648.195 kb on - strand at 648.217 kb on + strand at 648.237 kb on - strand at 648.241 kb on + strand at 648.285 kb on - strand at 648.288 kb on - strand at 648.309 kb on - strand at 648.685 kb on + strand at 648.691 kb on + strand at 648.695 kb on + strand at 648.703 kb on - strand at 648.710 kb on + strand at 648.841 kb on + strand, within BWI76_RS03150 at 648.867 kb on - strand, within BWI76_RS03150 at 648.869 kb on + strand, within BWI76_RS03150 at 648.877 kb on - strand, within BWI76_RS03150 at 648.877 kb on - strand, within BWI76_RS03150 at 648.906 kb on + strand, within BWI76_RS03150 at 648.906 kb on + strand, within BWI76_RS03150 at 648.913 kb on + strand, within BWI76_RS03150 at 648.921 kb on - strand, within BWI76_RS03150 at 648.963 kb on + strand, within BWI76_RS03150 at 649.056 kb on - strand, within BWI76_RS03150 at 649.097 kb on + strand, within BWI76_RS03150 at 649.097 kb on + strand, within BWI76_RS03150 at 649.097 kb on + strand, within BWI76_RS03150 at 649.112 kb on + strand, within BWI76_RS03150 at 649.151 kb on + strand, within BWI76_RS03150 at 649.159 kb on - strand, within BWI76_RS03150
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 646,254 - BWI76_RS03135 0.16 +1.5 646,254 - BWI76_RS03135 0.16 -0.4 646,321 + BWI76_RS03135 0.24 +1.0 646,421 + BWI76_RS03135 0.36 +1.3 646,524 - BWI76_RS03135 0.47 +0.5 646,541 + BWI76_RS03135 0.49 +1.5 646,598 + BWI76_RS03135 0.56 -1.1 646,606 - BWI76_RS03135 0.57 +0.1 646,606 - BWI76_RS03135 0.57 -3.3 646,606 - BWI76_RS03135 0.57 +0.0 646,639 + BWI76_RS03135 0.61 +0.4 646,647 + BWI76_RS03135 0.62 -0.5 646,768 + BWI76_RS03135 0.75 +0.0 646,768 + BWI76_RS03135 0.75 -0.0 646,881 - BWI76_RS03135 0.88 +0.1 646,887 + BWI76_RS03135 0.89 -0.0 647,206 + -3.8 647,260 - -0.4 647,274 + -3.9 647,378 - BWI76_RS03140 0.20 -2.0 647,610 + BWI76_RS03140 0.43 +0.3 647,632 - BWI76_RS03140 0.45 -0.0 647,891 + BWI76_RS03140 0.70 -0.9 647,899 - BWI76_RS03140 0.71 -0.0 647,945 + BWI76_RS03140 0.76 +1.5 647,954 + BWI76_RS03140 0.77 -1.1 647,954 + BWI76_RS03140 0.77 -0.0 647,992 + BWI76_RS03140 0.80 +1.5 647,992 + BWI76_RS03140 0.80 -1.4 648,090 - BWI76_RS03140 0.90 -0.2 648,195 - +0.9 648,217 + -1.3 648,237 - +0.1 648,241 + -0.9 648,285 - -1.1 648,288 - +0.8 648,309 - +1.1 648,685 + +0.6 648,691 + -0.5 648,695 + +0.5 648,703 - +0.1 648,710 + +1.0 648,841 + BWI76_RS03150 0.13 -0.5 648,867 - BWI76_RS03150 0.15 -0.8 648,869 + BWI76_RS03150 0.15 +0.6 648,877 - BWI76_RS03150 0.16 +0.2 648,877 - BWI76_RS03150 0.16 -3.2 648,906 + BWI76_RS03150 0.18 -2.5 648,906 + BWI76_RS03150 0.18 +0.5 648,913 + BWI76_RS03150 0.19 +0.5 648,921 - BWI76_RS03150 0.19 -3.8 648,963 + BWI76_RS03150 0.23 -0.8 649,056 - BWI76_RS03150 0.30 +1.5 649,097 + BWI76_RS03150 0.33 -2.8 649,097 + BWI76_RS03150 0.33 -0.8 649,097 + BWI76_RS03150 0.33 -1.1 649,112 + BWI76_RS03150 0.35 +1.5 649,151 + BWI76_RS03150 0.38 +1.5 649,159 - BWI76_RS03150 0.38 +0.4
Or see this region's nucleotide sequence