Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03065 and BWI76_RS03070 are separated by 361 nucleotides BWI76_RS03070 and BWI76_RS03075 are separated by 18 nucleotides
BWI76_RS03065: BWI76_RS03065 - hypothetical protein, at 628,017 to 628,838
_RS03065
BWI76_RS03070: BWI76_RS03070 - Methylmalonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.27) <small>(from data)</small>, at 629,200 to 630,705
_RS03070
BWI76_RS03075: BWI76_RS03075 - 5-deoxy-D-glucuronate isomerase (EC 5.3.1.30) <small>(from data)</small>, at 630,724 to 631,533
_RS03075
Position (kb) 629
630
631 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 628.225 kb on - strand, within BWI76_RS03065 at 628.343 kb on + strand, within BWI76_RS03065 at 628.375 kb on - strand, within BWI76_RS03065 at 628.385 kb on + strand, within BWI76_RS03065 at 628.595 kb on + strand, within BWI76_RS03065 at 628.603 kb on - strand, within BWI76_RS03065 at 628.610 kb on + strand, within BWI76_RS03065 at 628.625 kb on - strand, within BWI76_RS03065 at 628.625 kb on - strand, within BWI76_RS03065 at 628.634 kb on + strand, within BWI76_RS03065 at 628.727 kb on - strand, within BWI76_RS03065 at 628.738 kb on - strand, within BWI76_RS03065 at 628.743 kb on - strand, within BWI76_RS03065 at 628.745 kb on + strand, within BWI76_RS03065 at 629.169 kb on - strand at 629.169 kb on - strand at 629.445 kb on + strand, within BWI76_RS03070 at 629.641 kb on + strand, within BWI76_RS03070 at 629.832 kb on + strand, within BWI76_RS03070 at 629.837 kb on + strand, within BWI76_RS03070 at 629.840 kb on - strand, within BWI76_RS03070 at 629.845 kb on - strand, within BWI76_RS03070 at 629.947 kb on + strand, within BWI76_RS03070 at 630.110 kb on - strand, within BWI76_RS03070 at 630.152 kb on + strand, within BWI76_RS03070 at 630.239 kb on + strand, within BWI76_RS03070 at 630.394 kb on + strand, within BWI76_RS03070 at 630.516 kb on + strand, within BWI76_RS03070 at 630.517 kb on - strand, within BWI76_RS03070 at 630.819 kb on - strand, within BWI76_RS03075 at 630.869 kb on + strand, within BWI76_RS03075 at 630.899 kb on - strand, within BWI76_RS03075 at 630.926 kb on + strand, within BWI76_RS03075 at 630.990 kb on + strand, within BWI76_RS03075 at 630.990 kb on + strand, within BWI76_RS03075 at 630.998 kb on - strand, within BWI76_RS03075 at 631.193 kb on - strand, within BWI76_RS03075 at 631.350 kb on + strand, within BWI76_RS03075 at 631.367 kb on + strand, within BWI76_RS03075 at 631.471 kb on + strand at 631.508 kb on + strand at 631.639 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 628,225 - BWI76_RS03065 0.25 -0.7 628,343 + BWI76_RS03065 0.40 -0.2 628,375 - BWI76_RS03065 0.44 +1.3 628,385 + BWI76_RS03065 0.45 +1.0 628,595 + BWI76_RS03065 0.70 +1.8 628,603 - BWI76_RS03065 0.71 +0.4 628,610 + BWI76_RS03065 0.72 +0.7 628,625 - BWI76_RS03065 0.74 +0.1 628,625 - BWI76_RS03065 0.74 -1.3 628,634 + BWI76_RS03065 0.75 -2.3 628,727 - BWI76_RS03065 0.86 -0.0 628,738 - BWI76_RS03065 0.88 -0.9 628,743 - BWI76_RS03065 0.88 -1.3 628,745 + BWI76_RS03065 0.89 -0.1 629,169 - -1.0 629,169 - -1.9 629,445 + BWI76_RS03070 0.16 +1.1 629,641 + BWI76_RS03070 0.29 -0.7 629,832 + BWI76_RS03070 0.42 +1.5 629,837 + BWI76_RS03070 0.42 -0.6 629,840 - BWI76_RS03070 0.42 -0.5 629,845 - BWI76_RS03070 0.43 -2.8 629,947 + BWI76_RS03070 0.50 +0.0 630,110 - BWI76_RS03070 0.60 -2.6 630,152 + BWI76_RS03070 0.63 -0.5 630,239 + BWI76_RS03070 0.69 +0.3 630,394 + BWI76_RS03070 0.79 +0.1 630,516 + BWI76_RS03070 0.87 +0.3 630,517 - BWI76_RS03070 0.87 -2.6 630,819 - BWI76_RS03075 0.12 +0.1 630,869 + BWI76_RS03075 0.18 +0.6 630,899 - BWI76_RS03075 0.22 -0.4 630,926 + BWI76_RS03075 0.25 +4.3 630,990 + BWI76_RS03075 0.33 +0.6 630,990 + BWI76_RS03075 0.33 -0.0 630,998 - BWI76_RS03075 0.34 -0.1 631,193 - BWI76_RS03075 0.58 -0.0 631,350 + BWI76_RS03075 0.77 -0.1 631,367 + BWI76_RS03075 0.79 +0.2 631,471 + -0.3 631,508 + -0.0 631,639 - +0.2
Or see this region's nucleotide sequence