Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03010 and BWI76_RS03015 are separated by 164 nucleotides BWI76_RS03015 and BWI76_RS03020 are separated by 52 nucleotides BWI76_RS03020 and BWI76_RS03025 are separated by 99 nucleotides
BWI76_RS03010: BWI76_RS03010 - ornithine carbamoyltransferase, at 615,898 to 616,902
_RS03010
BWI76_RS03015: BWI76_RS03015 - RNase E inhibitor protein, at 617,067 to 617,492
_RS03015
BWI76_RS03020: BWI76_RS03020 - tRNA hydroxylase, at 617,545 to 618,303
_RS03020
BWI76_RS03025: BWI76_RS03025 - N-acetyltransferase, at 618,403 to 618,906
_RS03025
Position (kb) 617
618 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 616.124 kb on - strand, within BWI76_RS03010 at 616.214 kb on - strand, within BWI76_RS03010 at 616.216 kb on + strand, within BWI76_RS03010 at 616.224 kb on - strand, within BWI76_RS03010 at 616.361 kb on - strand, within BWI76_RS03010 at 616.361 kb on - strand, within BWI76_RS03010 at 616.388 kb on + strand, within BWI76_RS03010 at 616.423 kb on + strand, within BWI76_RS03010 at 616.431 kb on - strand, within BWI76_RS03010 at 616.480 kb on - strand, within BWI76_RS03010 at 616.480 kb on - strand, within BWI76_RS03010 at 616.489 kb on + strand, within BWI76_RS03010 at 616.507 kb on - strand, within BWI76_RS03010 at 616.530 kb on + strand, within BWI76_RS03010 at 616.695 kb on + strand, within BWI76_RS03010 at 616.808 kb on + strand at 616.808 kb on + strand at 616.808 kb on + strand at 616.808 kb on + strand at 616.808 kb on + strand at 616.816 kb on - strand at 616.844 kb on - strand at 616.906 kb on - strand at 617.167 kb on - strand, within BWI76_RS03015 at 617.323 kb on + strand, within BWI76_RS03015 at 617.607 kb on - strand at 617.654 kb on - strand, within BWI76_RS03020 at 617.665 kb on - strand, within BWI76_RS03020 at 617.665 kb on - strand, within BWI76_RS03020 at 617.761 kb on - strand, within BWI76_RS03020 at 617.995 kb on - strand, within BWI76_RS03020 at 617.995 kb on - strand, within BWI76_RS03020 at 618.331 kb on - strand at 618.410 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 616,124 - BWI76_RS03010 0.22 -0.9 616,214 - BWI76_RS03010 0.31 -1.5 616,216 + BWI76_RS03010 0.32 -1.3 616,224 - BWI76_RS03010 0.32 -0.5 616,361 - BWI76_RS03010 0.46 +0.2 616,361 - BWI76_RS03010 0.46 -0.1 616,388 + BWI76_RS03010 0.49 -0.0 616,423 + BWI76_RS03010 0.52 -1.2 616,431 - BWI76_RS03010 0.53 -1.9 616,480 - BWI76_RS03010 0.58 -0.9 616,480 - BWI76_RS03010 0.58 -2.9 616,489 + BWI76_RS03010 0.59 -1.6 616,507 - BWI76_RS03010 0.61 -1.0 616,530 + BWI76_RS03010 0.63 -0.8 616,695 + BWI76_RS03010 0.79 -0.1 616,808 + -0.1 616,808 + +0.9 616,808 + -3.0 616,808 + +2.0 616,808 + +1.7 616,816 - +0.3 616,844 - -1.3 616,906 - +1.6 617,167 - BWI76_RS03015 0.23 -0.8 617,323 + BWI76_RS03015 0.60 -3.4 617,607 - -0.7 617,654 - BWI76_RS03020 0.14 +0.5 617,665 - BWI76_RS03020 0.16 -1.3 617,665 - BWI76_RS03020 0.16 +0.4 617,761 - BWI76_RS03020 0.28 +0.7 617,995 - BWI76_RS03020 0.59 -0.1 617,995 - BWI76_RS03020 0.59 +1.5 618,331 - +2.6 618,410 + +0.4
Or see this region's nucleotide sequence