Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02850 and BWI76_RS02855 are separated by 36 nucleotides BWI76_RS02855 and BWI76_RS02860 are separated by 419 nucleotides BWI76_RS02860 and BWI76_RS02865 are separated by 11 nucleotides BWI76_RS02865 and BWI76_RS02870 are separated by 2 nucleotides BWI76_RS02870 and BWI76_RS02875 are separated by 21 nucleotides
BWI76_RS02850: BWI76_RS02850 - metalloprotease PmbA, at 583,827 to 585,179
_RS02850
BWI76_RS02855: BWI76_RS02855 - cytochrome B562, at 585,216 to 585,602
_RS02855
BWI76_RS02860: BWI76_RS02860 - glycine dehydrogenase, at 586,022 to 586,360
_RS02860
BWI76_RS02865: BWI76_RS02865 - hypothetical protein, at 586,372 to 586,734
_RS02865
BWI76_RS02870: BWI76_RS02870 - hypothetical protein, at 586,737 to 587,036
_RS02870
BWI76_RS02875: BWI76_RS02875 - membrane protein, at 587,058 to 587,834
_RS02875
Position (kb) 586
587 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 585.139 kb on + strand at 585.305 kb on - strand, within BWI76_RS02855 at 585.328 kb on - strand, within BWI76_RS02855 at 585.480 kb on - strand, within BWI76_RS02855 at 585.568 kb on - strand at 585.981 kb on + strand at 585.981 kb on + strand at 586.011 kb on - strand at 586.055 kb on - strand at 586.112 kb on + strand, within BWI76_RS02860 at 586.296 kb on - strand, within BWI76_RS02860 at 586.357 kb on + strand at 586.410 kb on - strand, within BWI76_RS02865 at 586.553 kb on + strand, within BWI76_RS02865 at 586.553 kb on + strand, within BWI76_RS02865 at 586.560 kb on + strand, within BWI76_RS02865 at 586.566 kb on - strand, within BWI76_RS02865 at 586.639 kb on + strand, within BWI76_RS02865 at 586.654 kb on + strand, within BWI76_RS02865 at 586.704 kb on - strand at 586.819 kb on - strand, within BWI76_RS02870 at 586.881 kb on - strand, within BWI76_RS02870 at 586.963 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 585,139 + -0.8 585,305 - BWI76_RS02855 0.23 -0.5 585,328 - BWI76_RS02855 0.29 -1.8 585,480 - BWI76_RS02855 0.68 +1.5 585,568 - -2.9 585,981 + +0.4 585,981 + -1.1 586,011 - +1.0 586,055 - +0.5 586,112 + BWI76_RS02860 0.27 +1.5 586,296 - BWI76_RS02860 0.81 +0.6 586,357 + -3.4 586,410 - BWI76_RS02865 0.10 +3.2 586,553 + BWI76_RS02865 0.50 -0.0 586,553 + BWI76_RS02865 0.50 -0.4 586,560 + BWI76_RS02865 0.52 +1.4 586,566 - BWI76_RS02865 0.53 -0.2 586,639 + BWI76_RS02865 0.74 +0.4 586,654 + BWI76_RS02865 0.78 -0.5 586,704 - -1.6 586,819 - BWI76_RS02870 0.27 -0.0 586,881 - BWI76_RS02870 0.48 -0.4 586,963 + +1.5
Or see this region's nucleotide sequence