Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02445 and BWI76_RS02450 are separated by 10 nucleotides BWI76_RS02450 and BWI76_RS02455 overlap by 8 nucleotides BWI76_RS02455 and BWI76_RS02460 are separated by 275 nucleotides
BWI76_RS02445: BWI76_RS02445 - fumarate reductase subunit C, at 500,534 to 500,929
_RS02445
BWI76_RS02450: BWI76_RS02450 - succinate dehydrogenase/fumarate reductase iron-sulfur subunit, at 500,940 to 501,674
_RS02450
BWI76_RS02455: BWI76_RS02455 - fumarate reductase flavoprotein subunit, at 501,667 to 503,457
_RS02455
BWI76_RS02460: BWI76_RS02460 - elongation factor P lysine(34) lysyltransferase, at 503,733 to 504,710
_RS02460
Position (kb) 501
502
503
504 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 500.762 kb on + strand, within BWI76_RS02445 at 500.848 kb on + strand, within BWI76_RS02445 at 500.850 kb on + strand, within BWI76_RS02445 at 500.911 kb on - strand at 500.948 kb on + strand at 500.956 kb on - strand at 500.994 kb on - strand at 501.287 kb on + strand, within BWI76_RS02450 at 501.420 kb on - strand, within BWI76_RS02450 at 501.475 kb on - strand, within BWI76_RS02450 at 501.505 kb on + strand, within BWI76_RS02450 at 501.552 kb on - strand, within BWI76_RS02450 at 501.601 kb on + strand at 501.601 kb on + strand at 501.688 kb on + strand at 501.874 kb on - strand, within BWI76_RS02455 at 501.941 kb on + strand, within BWI76_RS02455 at 501.941 kb on + strand, within BWI76_RS02455 at 501.949 kb on - strand, within BWI76_RS02455 at 501.949 kb on - strand, within BWI76_RS02455 at 501.964 kb on - strand, within BWI76_RS02455 at 501.974 kb on - strand, within BWI76_RS02455 at 502.086 kb on + strand, within BWI76_RS02455 at 502.122 kb on - strand, within BWI76_RS02455 at 502.305 kb on - strand, within BWI76_RS02455 at 502.381 kb on + strand, within BWI76_RS02455 at 502.439 kb on + strand, within BWI76_RS02455 at 502.483 kb on - strand, within BWI76_RS02455 at 502.493 kb on - strand, within BWI76_RS02455 at 502.529 kb on - strand, within BWI76_RS02455 at 502.552 kb on + strand, within BWI76_RS02455 at 502.553 kb on + strand, within BWI76_RS02455 at 502.577 kb on + strand, within BWI76_RS02455 at 502.607 kb on - strand, within BWI76_RS02455 at 503.038 kb on - strand, within BWI76_RS02455 at 503.087 kb on + strand, within BWI76_RS02455 at 503.144 kb on + strand, within BWI76_RS02455 at 503.153 kb on + strand, within BWI76_RS02455 at 503.313 kb on - strand at 503.319 kb on + strand at 503.409 kb on - strand at 503.414 kb on - strand at 503.513 kb on + strand at 503.658 kb on - strand at 503.658 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 500,762 + BWI76_RS02445 0.58 -0.8 500,848 + BWI76_RS02445 0.79 +0.6 500,850 + BWI76_RS02445 0.80 -0.5 500,911 - -0.3 500,948 + -2.5 500,956 - -1.3 500,994 - +0.3 501,287 + BWI76_RS02450 0.47 -3.8 501,420 - BWI76_RS02450 0.65 +0.2 501,475 - BWI76_RS02450 0.73 +0.8 501,505 + BWI76_RS02450 0.77 -0.1 501,552 - BWI76_RS02450 0.83 +0.7 501,601 + -2.5 501,601 + -1.7 501,688 + -0.3 501,874 - BWI76_RS02455 0.12 -0.7 501,941 + BWI76_RS02455 0.15 +0.9 501,941 + BWI76_RS02455 0.15 -0.2 501,949 - BWI76_RS02455 0.16 +0.2 501,949 - BWI76_RS02455 0.16 +0.2 501,964 - BWI76_RS02455 0.17 -0.5 501,974 - BWI76_RS02455 0.17 +1.5 502,086 + BWI76_RS02455 0.23 -3.5 502,122 - BWI76_RS02455 0.25 -0.8 502,305 - BWI76_RS02455 0.36 +1.8 502,381 + BWI76_RS02455 0.40 -2.6 502,439 + BWI76_RS02455 0.43 -1.1 502,483 - BWI76_RS02455 0.46 -0.5 502,493 - BWI76_RS02455 0.46 +0.5 502,529 - BWI76_RS02455 0.48 -0.7 502,552 + BWI76_RS02455 0.49 -0.0 502,553 + BWI76_RS02455 0.49 +0.5 502,577 + BWI76_RS02455 0.51 +0.6 502,607 - BWI76_RS02455 0.52 +1.2 503,038 - BWI76_RS02455 0.77 -0.8 503,087 + BWI76_RS02455 0.79 -3.2 503,144 + BWI76_RS02455 0.82 -2.0 503,153 + BWI76_RS02455 0.83 +0.9 503,313 - -0.1 503,319 + +1.4 503,409 - -2.0 503,414 - +0.5 503,513 + +0.2 503,658 - +0.4 503,658 - +0.8
Or see this region's nucleotide sequence