Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02195 and BWI76_RS02200 overlap by 4 nucleotides BWI76_RS02200 and BWI76_RS02205 are separated by 196 nucleotides BWI76_RS02205 and BWI76_RS02210 overlap by 4 nucleotides
BWI76_RS02195: BWI76_RS02195 - alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase, at 454,745 to 455,881
_RS02195
BWI76_RS02200: BWI76_RS02200 - phosphonate C-P lyase system protein PhnL, at 455,878 to 456,564
_RS02200
BWI76_RS02205: BWI76_RS02205 - phosphonate C-P lyase system protein PhnK, at 456,761 to 457,519
_RS02205
BWI76_RS02210: BWI76_RS02210 - carbon-phosphorus lyase complex subunit PhnJ, at 457,516 to 458,364
_RS02210
Position (kb) 455
456
457 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 454.920 kb on - strand at 454.931 kb on + strand, within BWI76_RS02195 at 455.083 kb on - strand, within BWI76_RS02195 at 455.091 kb on - strand, within BWI76_RS02195 at 455.298 kb on - strand, within BWI76_RS02195 at 455.367 kb on + strand, within BWI76_RS02195 at 455.479 kb on + strand, within BWI76_RS02195 at 455.618 kb on - strand, within BWI76_RS02195 at 455.656 kb on - strand, within BWI76_RS02195 at 455.667 kb on + strand, within BWI76_RS02195 at 455.680 kb on - strand, within BWI76_RS02195 at 455.860 kb on + strand at 455.860 kb on + strand at 455.868 kb on - strand at 455.904 kb on + strand at 456.015 kb on - strand, within BWI76_RS02200 at 456.016 kb on + strand, within BWI76_RS02200 at 456.268 kb on - strand, within BWI76_RS02200 at 456.309 kb on - strand, within BWI76_RS02200 at 456.316 kb on - strand, within BWI76_RS02200 at 456.316 kb on - strand, within BWI76_RS02200 at 456.326 kb on - strand, within BWI76_RS02200 at 456.439 kb on + strand, within BWI76_RS02200 at 456.648 kb on - strand at 456.716 kb on + strand at 456.724 kb on - strand at 456.747 kb on + strand at 456.795 kb on + strand at 456.795 kb on + strand at 456.803 kb on - strand at 456.931 kb on + strand, within BWI76_RS02205 at 457.034 kb on + strand, within BWI76_RS02205 at 457.064 kb on - strand, within BWI76_RS02205 at 457.074 kb on - strand, within BWI76_RS02205 at 457.164 kb on + strand, within BWI76_RS02205 at 457.164 kb on + strand, within BWI76_RS02205 at 457.194 kb on - strand, within BWI76_RS02205 at 457.436 kb on + strand, within BWI76_RS02205 at 457.473 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 454,920 - +0.5 454,931 + BWI76_RS02195 0.16 +0.4 455,083 - BWI76_RS02195 0.30 -2.1 455,091 - BWI76_RS02195 0.30 -1.3 455,298 - BWI76_RS02195 0.49 -1.1 455,367 + BWI76_RS02195 0.55 +0.8 455,479 + BWI76_RS02195 0.65 -2.0 455,618 - BWI76_RS02195 0.77 -1.4 455,656 - BWI76_RS02195 0.80 +1.5 455,667 + BWI76_RS02195 0.81 +0.2 455,680 - BWI76_RS02195 0.82 +0.2 455,860 + +0.2 455,860 + -2.3 455,868 - +0.0 455,904 + +0.8 456,015 - BWI76_RS02200 0.20 +0.0 456,016 + BWI76_RS02200 0.20 -2.1 456,268 - BWI76_RS02200 0.57 -2.1 456,309 - BWI76_RS02200 0.63 -1.0 456,316 - BWI76_RS02200 0.64 -1.5 456,316 - BWI76_RS02200 0.64 -1.4 456,326 - BWI76_RS02200 0.65 -3.3 456,439 + BWI76_RS02200 0.82 -2.0 456,648 - +0.0 456,716 + -2.3 456,724 - +0.4 456,747 + -0.0 456,795 + -2.8 456,795 + -0.3 456,803 - -0.7 456,931 + BWI76_RS02205 0.22 +1.9 457,034 + BWI76_RS02205 0.36 +0.8 457,064 - BWI76_RS02205 0.40 -0.4 457,074 - BWI76_RS02205 0.41 +0.5 457,164 + BWI76_RS02205 0.53 +0.8 457,164 + BWI76_RS02205 0.53 +0.9 457,194 - BWI76_RS02205 0.57 -2.1 457,436 + BWI76_RS02205 0.89 -0.4 457,473 + +0.8
Or see this region's nucleotide sequence