Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01530 and BWI76_RS01535 overlap by 4 nucleotides BWI76_RS01535 and BWI76_RS01540 are separated by 1 nucleotides BWI76_RS01540 and BWI76_RS01545 overlap by 17 nucleotides BWI76_RS01545 and BWI76_RS01550 overlap by 8 nucleotides
BWI76_RS01530: BWI76_RS01530 - 2-iminoacetate synthase ThiH, at 312,554 to 313,687
_RS01530
BWI76_RS01535: BWI76_RS01535 - thiazole synthase, at 313,684 to 314,454
_RS01535
BWI76_RS01540: BWI76_RS01540 - thiamine biosynthesis protein ThiS, at 314,456 to 314,656
_RS01540
BWI76_RS01545: BWI76_RS01545 - thiamine biosynthesis protein ThiS, at 314,640 to 315,395
_RS01545
BWI76_RS01550: BWI76_RS01550 - thiamine phosphate synthase, at 315,388 to 316,023
_RS01550
Position (kb) 314
315 Strain fitness (log2 ratio) -2
-1
0
1
2 at 313.466 kb on + strand, within BWI76_RS01530 at 313.636 kb on + strand at 313.636 kb on + strand at 313.716 kb on + strand at 313.846 kb on + strand at 313.849 kb on - strand, within BWI76_RS01535 at 313.908 kb on - strand, within BWI76_RS01535 at 314.057 kb on - strand, within BWI76_RS01535 at 314.068 kb on - strand, within BWI76_RS01535 at 314.080 kb on + strand, within BWI76_RS01535 at 314.178 kb on + strand, within BWI76_RS01535 at 314.257 kb on - strand, within BWI76_RS01535 at 314.337 kb on + strand, within BWI76_RS01535 at 314.624 kb on + strand, within BWI76_RS01540 at 314.624 kb on + strand, within BWI76_RS01540 at 314.743 kb on + strand, within BWI76_RS01545 at 315.138 kb on - strand, within BWI76_RS01545 at 315.202 kb on - strand, within BWI76_RS01545 at 315.435 kb on + strand at 315.461 kb on + strand, within BWI76_RS01550 at 315.551 kb on - strand, within BWI76_RS01550
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 313,466 + BWI76_RS01530 0.80 -0.9 313,636 + +0.7 313,636 + +0.3 313,716 + -0.8 313,846 + +0.5 313,849 - BWI76_RS01535 0.21 -0.5 313,908 - BWI76_RS01535 0.29 +1.6 314,057 - BWI76_RS01535 0.48 +1.0 314,068 - BWI76_RS01535 0.50 +0.6 314,080 + BWI76_RS01535 0.51 +0.2 314,178 + BWI76_RS01535 0.64 +0.3 314,257 - BWI76_RS01535 0.74 -0.8 314,337 + BWI76_RS01535 0.85 +0.0 314,624 + BWI76_RS01540 0.84 -1.7 314,624 + BWI76_RS01540 0.84 -2.6 314,743 + BWI76_RS01545 0.14 +2.3 315,138 - BWI76_RS01545 0.66 -0.1 315,202 - BWI76_RS01545 0.74 +2.0 315,435 + -0.5 315,461 + BWI76_RS01550 0.11 -2.2 315,551 - BWI76_RS01550 0.26 +0.9
Or see this region's nucleotide sequence