Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01250 and BWI76_RS01255 are separated by 246 nucleotides BWI76_RS01255 and BWI76_RS01260 are separated by 109 nucleotides BWI76_RS01260 and BWI76_RS01265 are separated by 43 nucleotides BWI76_RS01265 and BWI76_RS01270 are separated by 88 nucleotides
BWI76_RS01250: BWI76_RS01250 - threonine export protein RhtC, at 256,483 to 257,106
_RS01250
BWI76_RS01255: BWI76_RS01255 - homoserine/homoserine lactone efflux protein, at 257,353 to 257,973
_RS01255
BWI76_RS01260: BWI76_RS01260 - lysophospholipase L2, at 258,083 to 259,075
_RS01260
BWI76_RS01265: BWI76_RS01265 - sugar/pyridoxal phosphate phosphatase YigL, at 259,119 to 259,919
_RS01265
BWI76_RS01270: BWI76_RS01270 - hypothetical protein, at 260,008 to 260,907
_RS01270
Position (kb) 258
259
260 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 257.129 kb on + strand at 257.137 kb on - strand at 257.179 kb on + strand at 257.379 kb on - strand at 257.379 kb on - strand at 257.464 kb on - strand, within BWI76_RS01255 at 257.533 kb on + strand, within BWI76_RS01255 at 257.543 kb on + strand, within BWI76_RS01255 at 257.551 kb on - strand, within BWI76_RS01255 at 257.687 kb on - strand, within BWI76_RS01255 at 257.689 kb on + strand, within BWI76_RS01255 at 257.696 kb on + strand, within BWI76_RS01255 at 257.761 kb on + strand, within BWI76_RS01255 at 257.769 kb on - strand, within BWI76_RS01255 at 257.811 kb on + strand, within BWI76_RS01255 at 257.853 kb on - strand at 258.014 kb on - strand at 258.080 kb on - strand at 258.332 kb on - strand, within BWI76_RS01260 at 258.485 kb on + strand, within BWI76_RS01260 at 258.620 kb on + strand, within BWI76_RS01260 at 258.620 kb on + strand, within BWI76_RS01260 at 258.628 kb on - strand, within BWI76_RS01260 at 258.689 kb on + strand, within BWI76_RS01260 at 258.907 kb on - strand, within BWI76_RS01260 at 258.955 kb on - strand, within BWI76_RS01260 at 258.963 kb on + strand, within BWI76_RS01260 at 259.069 kb on + strand at 259.099 kb on + strand at 259.099 kb on + strand at 259.186 kb on + strand at 259.508 kb on - strand, within BWI76_RS01265 at 259.635 kb on + strand, within BWI76_RS01265 at 259.660 kb on + strand, within BWI76_RS01265 at 259.701 kb on - strand, within BWI76_RS01265 at 259.750 kb on - strand, within BWI76_RS01265 at 259.760 kb on + strand, within BWI76_RS01265 at 259.769 kb on - strand, within BWI76_RS01265 at 259.806 kb on + strand, within BWI76_RS01265 at 259.846 kb on - strand at 260.025 kb on - strand at 260.056 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 257,129 + -0.1 257,137 - +0.8 257,179 + -1.5 257,379 - +1.1 257,379 - +0.5 257,464 - BWI76_RS01255 0.18 -0.6 257,533 + BWI76_RS01255 0.29 -0.8 257,543 + BWI76_RS01255 0.31 +1.0 257,551 - BWI76_RS01255 0.32 +0.1 257,687 - BWI76_RS01255 0.54 -1.1 257,689 + BWI76_RS01255 0.54 -2.1 257,696 + BWI76_RS01255 0.55 -0.2 257,761 + BWI76_RS01255 0.66 +0.1 257,769 - BWI76_RS01255 0.67 +0.7 257,811 + BWI76_RS01255 0.74 +0.4 257,853 - +1.5 258,014 - -2.7 258,080 - +2.2 258,332 - BWI76_RS01260 0.25 +2.5 258,485 + BWI76_RS01260 0.40 -0.4 258,620 + BWI76_RS01260 0.54 -0.3 258,620 + BWI76_RS01260 0.54 -0.9 258,628 - BWI76_RS01260 0.55 +0.0 258,689 + BWI76_RS01260 0.61 +0.4 258,907 - BWI76_RS01260 0.83 -1.3 258,955 - BWI76_RS01260 0.88 +0.2 258,963 + BWI76_RS01260 0.89 -1.5 259,069 + -1.1 259,099 + +0.4 259,099 + -0.3 259,186 + +0.6 259,508 - BWI76_RS01265 0.49 +0.4 259,635 + BWI76_RS01265 0.64 +1.1 259,660 + BWI76_RS01265 0.68 +0.7 259,701 - BWI76_RS01265 0.73 -0.3 259,750 - BWI76_RS01265 0.79 +0.6 259,760 + BWI76_RS01265 0.80 +1.3 259,769 - BWI76_RS01265 0.81 +0.5 259,806 + BWI76_RS01265 0.86 +1.6 259,846 - +0.4 260,025 - +0.5 260,056 - -3.4
Or see this region's nucleotide sequence