Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00805 and BWI76_RS00810 are separated by 132 nucleotides BWI76_RS00810 and BWI76_RS00815 are separated by 138 nucleotides
BWI76_RS00805: BWI76_RS00805 - 5,10-methylenetetrahydrofolate reductase, at 164,983 to 165,870
_RS00805
BWI76_RS00810: BWI76_RS00810 - DeoR family transcriptional regulator, at 166,003 to 166,752
_RS00810
BWI76_RS00815: BWI76_RS00815 - glycerol dehydrogenase, at 166,891 to 167,994
_RS00815
Position (kb) 166
167 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 165.056 kb on - strand at 165.139 kb on - strand, within BWI76_RS00805 at 165.252 kb on - strand, within BWI76_RS00805 at 165.364 kb on - strand, within BWI76_RS00805 at 165.492 kb on + strand, within BWI76_RS00805 at 165.803 kb on + strand at 165.808 kb on + strand at 165.811 kb on - strand at 165.816 kb on - strand at 165.823 kb on + strand at 165.823 kb on + strand at 165.848 kb on + strand at 165.853 kb on + strand at 165.880 kb on + strand at 165.914 kb on + strand at 166.001 kb on + strand at 166.009 kb on - strand at 166.009 kb on - strand at 166.068 kb on - strand at 166.095 kb on + strand, within BWI76_RS00810 at 166.204 kb on - strand, within BWI76_RS00810 at 166.254 kb on + strand, within BWI76_RS00810 at 166.262 kb on - strand, within BWI76_RS00810 at 166.293 kb on - strand, within BWI76_RS00810 at 166.346 kb on - strand, within BWI76_RS00810 at 166.447 kb on - strand, within BWI76_RS00810 at 166.704 kb on + strand at 166.704 kb on + strand at 166.826 kb on + strand at 166.827 kb on + strand at 166.954 kb on - strand at 166.954 kb on - strand at 167.100 kb on + strand, within BWI76_RS00815 at 167.207 kb on + strand, within BWI76_RS00815 at 167.221 kb on + strand, within BWI76_RS00815 at 167.285 kb on - strand, within BWI76_RS00815 at 167.510 kb on + strand, within BWI76_RS00815 at 167.640 kb on + strand, within BWI76_RS00815 at 167.735 kb on + strand, within BWI76_RS00815 at 167.743 kb on - strand, within BWI76_RS00815
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 165,056 - -0.7 165,139 - BWI76_RS00805 0.18 -1.4 165,252 - BWI76_RS00805 0.30 -0.2 165,364 - BWI76_RS00805 0.43 -2.2 165,492 + BWI76_RS00805 0.57 +0.7 165,803 + +2.0 165,808 + +1.6 165,811 - +0.1 165,816 - +0.1 165,823 + +0.4 165,823 + +0.6 165,848 + -0.3 165,853 + +0.9 165,880 + -2.7 165,914 + +0.2 166,001 + -3.1 166,009 - -3.4 166,009 - +0.5 166,068 - +0.2 166,095 + BWI76_RS00810 0.12 +0.5 166,204 - BWI76_RS00810 0.27 +0.0 166,254 + BWI76_RS00810 0.33 +0.7 166,262 - BWI76_RS00810 0.35 +0.7 166,293 - BWI76_RS00810 0.39 +0.1 166,346 - BWI76_RS00810 0.46 +0.8 166,447 - BWI76_RS00810 0.59 +1.1 166,704 + -2.6 166,704 + -0.9 166,826 + -0.1 166,827 + +1.0 166,954 - -0.5 166,954 - +1.1 167,100 + BWI76_RS00815 0.19 -0.4 167,207 + BWI76_RS00815 0.29 -1.6 167,221 + BWI76_RS00815 0.30 +1.0 167,285 - BWI76_RS00815 0.36 -1.7 167,510 + BWI76_RS00815 0.56 -1.1 167,640 + BWI76_RS00815 0.68 -2.4 167,735 + BWI76_RS00815 0.76 -0.4 167,743 - BWI76_RS00815 0.77 +0.6
Or see this region's nucleotide sequence