Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00435 and BWI76_RS00440 are separated by 205 nucleotides BWI76_RS00440 and BWI76_RS00445 overlap by 4 nucleotides BWI76_RS00445 and BWI76_RS00450 are separated by 37 nucleotides
BWI76_RS00435: BWI76_RS00435 - lipase, at 90,170 to 91,087
_RS00435
BWI76_RS00440: BWI76_RS00440 - CopG family transcriptional regulator, at 91,293 to 91,532
_RS00440
BWI76_RS00445: BWI76_RS00445 - PIN domain nuclease, at 91,529 to 91,903
_RS00445
BWI76_RS00450: BWI76_RS00450 - formate dehydrogenase accessory protein FdhE, at 91,941 to 92,870
_RS00450
Position (kb) 91
92 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 90.301 kb on + strand, within BWI76_RS00435 at 90.323 kb on - strand, within BWI76_RS00435 at 90.477 kb on - strand, within BWI76_RS00435 at 90.560 kb on + strand, within BWI76_RS00435 at 90.578 kb on - strand, within BWI76_RS00435 at 90.585 kb on + strand, within BWI76_RS00435 at 90.611 kb on + strand, within BWI76_RS00435 at 90.619 kb on - strand, within BWI76_RS00435 at 90.641 kb on - strand, within BWI76_RS00435 at 90.670 kb on + strand, within BWI76_RS00435 at 90.805 kb on + strand, within BWI76_RS00435 at 90.813 kb on - strand, within BWI76_RS00435 at 91.063 kb on + strand at 91.279 kb on + strand at 91.308 kb on + strand at 91.364 kb on + strand, within BWI76_RS00440 at 91.645 kb on - strand, within BWI76_RS00445 at 91.693 kb on + strand, within BWI76_RS00445 at 91.746 kb on + strand, within BWI76_RS00445 at 91.783 kb on + strand, within BWI76_RS00445 at 91.800 kb on + strand, within BWI76_RS00445 at 91.807 kb on + strand, within BWI76_RS00445 at 91.807 kb on + strand, within BWI76_RS00445 at 91.807 kb on + strand, within BWI76_RS00445 at 91.815 kb on - strand, within BWI76_RS00445 at 91.830 kb on + strand, within BWI76_RS00445 at 91.830 kb on + strand, within BWI76_RS00445 at 91.830 kb on + strand, within BWI76_RS00445 at 91.934 kb on - strand at 91.934 kb on - strand at 91.970 kb on + strand at 91.978 kb on - strand at 92.014 kb on + strand at 92.222 kb on + strand, within BWI76_RS00450 at 92.226 kb on + strand, within BWI76_RS00450 at 92.251 kb on - strand, within BWI76_RS00450
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 90,301 + BWI76_RS00435 0.14 +0.4 90,323 - BWI76_RS00435 0.17 +2.4 90,477 - BWI76_RS00435 0.33 +0.6 90,560 + BWI76_RS00435 0.42 -0.2 90,578 - BWI76_RS00435 0.44 +2.6 90,585 + BWI76_RS00435 0.45 +0.2 90,611 + BWI76_RS00435 0.48 -0.8 90,619 - BWI76_RS00435 0.49 -3.5 90,641 - BWI76_RS00435 0.51 -0.4 90,670 + BWI76_RS00435 0.54 -0.8 90,805 + BWI76_RS00435 0.69 -1.0 90,813 - BWI76_RS00435 0.70 -2.1 91,063 + +1.5 91,279 + +1.3 91,308 + -0.4 91,364 + BWI76_RS00440 0.30 -1.8 91,645 - BWI76_RS00445 0.31 -0.6 91,693 + BWI76_RS00445 0.44 +1.7 91,746 + BWI76_RS00445 0.58 -0.3 91,783 + BWI76_RS00445 0.68 +0.2 91,800 + BWI76_RS00445 0.72 -0.9 91,807 + BWI76_RS00445 0.74 -0.2 91,807 + BWI76_RS00445 0.74 -2.8 91,807 + BWI76_RS00445 0.74 -1.4 91,815 - BWI76_RS00445 0.76 -1.9 91,830 + BWI76_RS00445 0.80 -2.6 91,830 + BWI76_RS00445 0.80 -0.5 91,830 + BWI76_RS00445 0.80 +0.9 91,934 - -2.1 91,934 - +1.5 91,970 + -1.3 91,978 - -0.6 92,014 + -0.2 92,222 + BWI76_RS00450 0.30 -2.2 92,226 + BWI76_RS00450 0.31 -1.9 92,251 - BWI76_RS00450 0.33 -1.0
Or see this region's nucleotide sequence