Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27640 and BWI76_RS27645 are separated by 169 nucleotides BWI76_RS27645 and BWI76_RS27650 overlap by 9 nucleotides BWI76_RS27650 and BWI76_RS27655 are separated by 24 nucleotides
BWI76_RS27640: BWI76_RS27640 - hypothetical protein, at 5,735,426 to 5,736,205
_RS27640
BWI76_RS27645: BWI76_RS27645 - ADP-ribose pyrophosphatase, at 5,736,375 to 5,736,929
_RS27645
BWI76_RS27650: BWI76_RS27650 - EamA family transporter, at 5,736,921 to 5,737,781
_RS27650
BWI76_RS27655: BWI76_RS27655 - lipoprotein NlpA, at 5,737,806 to 5,738,615
_RS27655
Position (kb) 5736
5737
5738 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5735.928 kb on + strand, within BWI76_RS27640 at 5735.955 kb on + strand, within BWI76_RS27640 at 5735.955 kb on + strand, within BWI76_RS27640 at 5736.003 kb on - strand, within BWI76_RS27640 at 5736.180 kb on - strand at 5736.188 kb on - strand at 5736.347 kb on - strand at 5736.361 kb on - strand at 5736.430 kb on + strand at 5736.438 kb on - strand, within BWI76_RS27645 at 5736.585 kb on - strand, within BWI76_RS27645 at 5736.727 kb on + strand, within BWI76_RS27645 at 5736.740 kb on + strand, within BWI76_RS27645 at 5736.762 kb on + strand, within BWI76_RS27645 at 5736.840 kb on - strand, within BWI76_RS27645 at 5736.960 kb on - strand at 5737.103 kb on - strand, within BWI76_RS27650 at 5737.135 kb on - strand, within BWI76_RS27650 at 5737.247 kb on + strand, within BWI76_RS27650 at 5737.255 kb on - strand, within BWI76_RS27650 at 5737.423 kb on - strand, within BWI76_RS27650 at 5737.474 kb on + strand, within BWI76_RS27650 at 5737.555 kb on - strand, within BWI76_RS27650 at 5737.574 kb on - strand, within BWI76_RS27650 at 5737.596 kb on + strand, within BWI76_RS27650 at 5737.752 kb on - strand at 5737.752 kb on - strand at 5737.838 kb on - strand at 5737.879 kb on - strand at 5737.888 kb on - strand, within BWI76_RS27655 at 5738.147 kb on + strand, within BWI76_RS27655 at 5738.289 kb on + strand, within BWI76_RS27655 at 5738.296 kb on + strand, within BWI76_RS27655 at 5738.340 kb on - strand, within BWI76_RS27655 at 5738.443 kb on - strand, within BWI76_RS27655 at 5738.490 kb on + strand, within BWI76_RS27655 at 5738.503 kb on + strand, within BWI76_RS27655 at 5738.518 kb on + strand, within BWI76_RS27655 at 5738.526 kb on - strand, within BWI76_RS27655 at 5738.526 kb on - strand, within BWI76_RS27655 at 5738.539 kb on + strand at 5738.565 kb on + strand at 5738.675 kb on + strand at 5738.775 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 5,735,928 + BWI76_RS27640 0.64 +0.3 5,735,955 + BWI76_RS27640 0.68 -0.6 5,735,955 + BWI76_RS27640 0.68 -1.0 5,736,003 - BWI76_RS27640 0.74 -1.1 5,736,180 - +0.3 5,736,188 - +1.0 5,736,347 - +0.3 5,736,361 - +0.7 5,736,430 + -0.2 5,736,438 - BWI76_RS27645 0.11 +0.3 5,736,585 - BWI76_RS27645 0.38 +0.0 5,736,727 + BWI76_RS27645 0.63 -0.1 5,736,740 + BWI76_RS27645 0.66 -0.3 5,736,762 + BWI76_RS27645 0.70 +0.3 5,736,840 - BWI76_RS27645 0.84 +0.2 5,736,960 - +1.0 5,737,103 - BWI76_RS27650 0.21 +1.3 5,737,135 - BWI76_RS27650 0.25 +0.0 5,737,247 + BWI76_RS27650 0.38 +0.3 5,737,255 - BWI76_RS27650 0.39 +0.2 5,737,423 - BWI76_RS27650 0.58 +1.5 5,737,474 + BWI76_RS27650 0.64 +0.6 5,737,555 - BWI76_RS27650 0.74 +1.1 5,737,574 - BWI76_RS27650 0.76 +2.6 5,737,596 + BWI76_RS27650 0.78 +1.2 5,737,752 - -3.1 5,737,752 - +0.1 5,737,838 - -0.2 5,737,879 - +0.3 5,737,888 - BWI76_RS27655 0.10 -0.3 5,738,147 + BWI76_RS27655 0.42 -0.7 5,738,289 + BWI76_RS27655 0.60 +0.5 5,738,296 + BWI76_RS27655 0.60 -1.3 5,738,340 - BWI76_RS27655 0.66 +0.4 5,738,443 - BWI76_RS27655 0.79 +0.5 5,738,490 + BWI76_RS27655 0.84 +0.1 5,738,503 + BWI76_RS27655 0.86 +0.9 5,738,518 + BWI76_RS27655 0.88 -0.3 5,738,526 - BWI76_RS27655 0.89 +0.1 5,738,526 - BWI76_RS27655 0.89 +0.0 5,738,539 + +0.2 5,738,565 + +0.0 5,738,675 + -0.8 5,738,775 - +0.2
Or see this region's nucleotide sequence